In Vitro-Evaluation of the Antioxidant Properties of Moringa Oleifera and Camelia Sinensis Leaves

Abstract

IntroductionMoringa oleifera and Camellia sinensis are edible plants widely distributed in parts of South East of Nigeria. Both are used as a beverage and medicinal plant in the treatment of several ailments by alternative medical practitioners. The in vitro-antioxidant properties of both Moringa oleifera and Camelia sinensis leaves were investigated in the current study.. MethodPhytochemical analysis was carried out to evaluate the flavonoids and total phenolic contents of the plants crude extracts. Also, reducing power ability and nitric oxide scavenging activity of the plants were also determined in order to ascertain their antioxidant capacities to eliminate free radicals, and attenuate oxidative stress. ResultsBoth plant samples demonstrated antioxidant properties. The samples were rich in flavonoid (135.14±5.20 and 208.24±14.38), and total phenolics (62.85±1.70 and 91.68±0.22) for both Moringa oleifera and Camelia sinensis respectively. The plant samples also showed nitric oxide scavenging and reducing power ability. Camelia sinensis appears to be a better antioxidant plant. ConclusionMoringa oleifera and Camelia sinensis contains substantial amount of antioxidant substances which could warrant their utilization in alternative medicine. However, further research works are needed to properly ascertain and harness these bioactive agents present in the plants.

Moringa oleifera Lam. Commercial Beverages: A Multifaceted Investigation of Consumer Perceptions, Sensory Analysis, and Bioactive Properties

“…The obtained results demonstrated that the sample type influences the content of phenolic compounds recovered from moringa. Comparing our TPC values with available information in the literature, it was observed that results from the present study were at the same level as the values reported by Ilesanmi et al [39] for 80% aqueous methanolic extracts of M. oleifera leaves (541 ± 1 mg GAE/g dw), but higher than those reported by other researchers [40][41][42][43][44]. Indeed, our TPC findings were higher than those of M. oleifera trees found in the Menzel Lahbib (Gabes-Tunisia) region, whose values ranged from 80.9 ± 1.2 to 136 ± 8 mg GAE/g dw.…”

“…Ethyl acetate and chloroform fractions were the most active ones, containing the highest TPC but not the highest TFC. An 80% methanolic extract prepared with the leaves was also reported by Ayoola et al [40]. However, the extract did not scavenge the radical in a dose-response manner, displaying % inhibition in the range of 59.5% (at 6.25 µg/mL) and 50.4% (at 100 µg/mL).…”

“…There were, however, no significant differences (p > 0.05) between Samples 3 and 5 and between Samples 1 and 2. Unfortunately, a direct comparison of the obtained flavonoid content with data from the literature cannot be performed, as most of the studies employed other standards (such as rutin or quercetin) to express the TFC values [40][41][42][43]. However, a good correlation coefficient between the TPC and TFC assays was observed (r = 0.887, data not shown), which agreed with that previously reported by other authors for M. oleifera leaves [42,43], revealing that Sample 3 could be the most potent natural source of bioactive compounds.…”

Zogale (Moringaolifera) as a functional ingredient: A review on its nutraceutical properties and food applications

“…The potential of M. oleifera leaf extract has shown substantial morphological variations and decreased cell viability, with increased ROS generation and inter-nucleosomal DNA fragmentation in the KB cells. [48,49] The Moringa held control for the ROS production and seems to be mark oriented for continuously dividing cells. This could be further verified by the up ruling of caspase 9 and caspase 3 which are part of the apoptotic pathway.…”

Usage of Moringa Leaves as a Component in some Foodstuff

“…Moringa oleifera (MO) has been a perfect source of protein and antioxidants, unsaturated fatty acids furthermore vitamins. The nutritional, besides the medicinal characteristic of MO products, have recently appeal attention from various industries world (Ayoola et al, 2019).…”