In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates

Abstract

BackgroundThis study aims to assess the susceptibility of Acinetobacter baumannii isolates to the antiseptics and disinfectants commonly used, and to the non-approved product.MethodsThis is a prospective study carried out from February to August 2015, in the Bacteriology department of Mohammed V Military Teaching hospital of Rabat on A.baumannii isolates collected from colonized and/or infected patients and environmental samples. The antiseptics and disinfectants susceptibility testing was assessed using the micromethod validated in our department. The antiseptics and disinfectants studied were: 70% ethyl alcohol, chlorhexidine, povidone-iodine, didecyldimethylammonium chloride and a commercial product which was presented as a hospital disinfectant (non-registered product).ResultsPovidone-iodine, 0.5% chlorhexidine digluconate, 70% ethyl alcohol and didecyl dimethyl ammonium chloride in combination with N- (3-aminopropyl) -N-dodecylpropane-1, 3-diamine were effective against all the 81 A.baumannii isolates tested, and their logarithmic reduction ≥ 5 were observed in 100% of the isolates in their undiluted form. The strains isolated from patients were more resistant than environmental strains: at a dilution of ½ for 70% ethyl alcohol (37.77% vs 11.11%, p = 0.007) and at a dilution of 1/10 (100% vs 69.44%, p < 0.001) for povidone iodine. The non-registered product was ineffective with a resistance rate of 96.29% at a dilution of 1/50, 45.67% at a dilution of 1/10 and 13.58% in its purest form.ConclusionOur study revealed the effectiveness of the main disinfectants and antiseptics used in Morocco; three antiseptics tested were effective in their purest form against the 81 A.baumannii isolates. Regarding disinfectants, our results showed an efficacy of didecyl dimethyl ammonium at the recommended use concentration and in its purest form. This study emphasizes the need for using disinfectants and antiseptics in dilutions recommended by the manufacturer because the insufficient dilutions of these products are not effective. Our findings also demonstrated an inefficiency of the non-registered product against A.baumanii isolates. However, the non-registered products should be prohibited.

An Herbal Alternative to Control Nosocomial Pathogens in Aerosols and Splatter During Ultrasonic Scaling

“…baumannii were well in agreement with two in vitro studies [21,22]. On the contrary, the results with regard to MRSA were not in concurrence with an earlier study [23] who has shown a better efficacy for chlorhexidine against MRSA.…”

Molecular Characterization of Reduced Susceptibility to Biocides in Clinical Isolates of Acinetobacter baumannii

“…Another report also showed the spread of carbapenem-resistant A. baumannii belonging to CC92 (Chen et al, 2017 ); however, biocide susceptibility phenotypes were not assessed. Together with the antibiotic and biocide susceptibility phenotypes of these clones we studied, the present study continues to argue the importance to monitor the biocide susceptibility to A. baumannii and to promote biocide stewardship in hospitals (Kampf, 2016 ; Lanjri et al, 2017 ).…”

In Vitro Bactericidal and Virucidal Efficacy of Povidone-Iodine Gargle/Mouthwash Against Respiratory and Oral Tract Pathogens

“…In addition, these species represent both gram-positive and -negative bacteria. Although our study did not include other common bacterial pathogens causing pneumonia such as Staphylococcus aureus , Pseudomonas aeruginosa , Haemophilus influenzae and Acinetobacter baumannii , the efficacy of PVP-I against these pathogens has already been demonstrated in previous studies [ 16 , 18 , 32 , 33 , 41 , 46 ].…”