In Vitro Evaluation of the Biosafety of Hyaluronic Acid PEG Cross-Linked with Micromolecules of Calcium Hydroxyapatite in Low Concentration

Zerbinati, Nicola; Lotti, Torello; Monticelli, Damiano; Rauso, Raffaele; González-Isaza, Pablo; D’Este, Edoardo; Calligaro, Alberto; Sommatis, Sabrina; Maccario, Cristina; Mocchi, Roberto; Lotti, Jacopo; Wollina, Uwe; Tchernev, Georgi; França, Katlein

doi:10.3889/oamjms.2018.044

Cited by 26 publications

(42 citation statements)

References 13 publications

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“…PEG is generally considered to have low toxicity by all routes of administration 18 : its metabolism was reviewed by Webster et al 19 The molecular weight of PEGD is not known. In general, PEG is not considered biodegradable except for low molecular weight species (<400 Da) that may be degraded by alcohol dehydrogenase in vivo 5,6,19‐32 …”

Section: Introductionmentioning

confidence: 99%

“…In general, PEG is not considered biodegradable except for low molecular weight species (<400 Da) that may be degraded by alcohol dehydrogenase in vivo. 5,6,[19][20][21][22][23][24][25][26][27][28][29][30][31][32] Its application as a crosslinker for HA hydrogels stems from the combination of its low toxicity and the good rheological properties of PEG crosslinked hydrogels. 33 The aim of this study was to evaluate the chemical, viscoelastic and biological properties of a recently introduced hydrogel based on PEG-crosslinked HA with a high concentration of HA (28 mg/mL), commercialized as Neauvia Intense (MatexLab, SA).…”

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confidence: 99%

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Chemical and mechanical characterization of hyaluronic acid hydrogel cross‐linked with polyethylen glycol and its use in dermatology

Zerbinati

Esposito²,

Cipolla³

et al. 2020

Dermatologic Therapy

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Hydrogels based on hyaluronic acid are used to restore volume, hydration, and skin tone, as well as to correct scars, asymmetries or defects of the soft tissue. Hyaluronic acid is often chemically crosslinked with different crosslinking agents in order to improve its mechanical and biological properties. Here we focused on defining the chemical and mechanical characterization of a new hydrogel with specific characteristics: hyaluronic acid polyethylene glycol (PEG)‐crosslinked with a high concentration of hyaluronic acid (28 mg/mL), manufactured by MatexLab Spa, via Carlo Urbani 2, ang Via Enrico Fermi, Brindisi, Italy. We made a quantitative and qualitative analysis of the content of sodium hyaluronate in the hydrogel after polymerization and sterilization processes and also evaluated histologically the bio integration of these hydrogels in the cutaneous soft tissues. The results suggest that hyaluronic acid hydrogel PEG‐crosslinked have great bio integration, great chemical and mechanical properties, compared with other products available on the market, that are cross‐linked with different cross‐linking agents. The nontoxicity and nonimmunogenicity of PEG guarantee the lack of allergic and immunological reactions. The PEG‐crosslinking technology guarantees a high duration time of the implanted hydrogel because of more resistant physiological degradation.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Chemical and mechanical characterization of hyaluronic acid hydrogel cross‐linked with polyethylen glycol and its use in dermatology

Zerbinati

Esposito²,

Cipolla³

et al. 2020

Dermatologic Therapy

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“…Our results concerning the ability of N‐Gel to modulate human PMN functions, including migration, oxidative metabolism, and TNF alpha and IL‐8 expression, suggests that N‐Gel carries a very low risk of immune‐mediated adverse effects, particularly granulomatous reaction and associated cellulitic processes. In dermal cosmetics, as well as in aesthetic medicine, the use of products not only devoid of proinflammatory effects but also able to modulate any local inflammatory process, represents a significant opportunity, thus strengthening previous evidence and supporting N‐Gel as new frontier in dermal cosmetic 5,6,8‐10 …”

Section: Discussionmentioning

confidence: 99%

“…N‐Gel can be considered completely biocompatible and degradable hydrogel. The chemical properties and techniques used to obtain N‐Gel are reported in the literature 4,7,8,10,16 …”

Section: Methodsmentioning

confidence: 99%

Immune profile of hyaluronic acid hydrogel polyethylene glycol crosslinked: An in vitro evaluation in human polymorphonuclear leukocytes

Marino

Cosentino

Legnaro

et al. 2020

Dermatologic Therapy

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Neauvia hydrogel (N‐Gel) is a hyaluronic acid‐based dermal filler, cross‐linked with polyethylene glycol. This filler contains sodium hyaluronate at different concentrations, poly(ethylene glycol) diglycidyl ether cross‐linked, glycine, and l‐prolyne. Assessing any effects of N‐Gel on immunity and inflammation is of crucial importance. The aim of the study was to characterize the ability of N‐Gel to modulate human polymorphonuclear leukocyte (PMN) functions, including migration, oxidative metabolism, and production of proinflammatory mediators. N‐Gel was tested on isolated human PMN. Spontaneous and N‐formylmethionyl‐leucyl‐phenylalanine (fMLP)‐stimulated migration were examined using the Boyden Chamber technique, whereas the oxidative metabolism was assessed through spectrofluorometric measurement of reactive oxygen species (ROS) production under resting conditions and after stimulation with fMLP. Tumor necrosis factor (TNF)‐α and interleukin (IL)‐8 mRNA levels were measured by real‐time PCR after stimulation with fMLP or Escherichia coli lipopolysaccharide. This study showed that N‐Gel reduced fMLP‐induced migration and ROS production without affecting these functions in resting cells. In addition, incubation of PMN with N‐Gel effectively reduced both TNF‐α and IL‐8 mRNA levels. N‐Gel modulates critical functions of human PMN such as migration and oxidative metabolism, indicating its potential as an anti‐inflammatory agent.

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“…Hyaluronic acid hydrogel crosslinked with polyethene glycol (PEG) has been recently introduced [12], [14], [15]. Several features of these materials have already been investigated, namely the properties directly involved in the safe use as dermal fillers (degradation by hyaluronidase [16] and biosafety [17]).…”

Section: Introductionmentioning

confidence: 99%

Chemical Characterization of Hydrogels Crosslinked with Polyethylene Glycol for Soft Tissue Augmentation

Monticelli¹,

Martina²,

Mocchi³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND: Hyaluronic acid (HA) based hydrogels for esthetic applications found widespread use. HA should be crosslinked for this application to achieve the correct viscoelastic properties and avoid fast degradation by the hyaluronidase enzyme naturally present in the skin: these properties are controlled by the amount of crosslinker and the fraction that is effectively crosslinked (i.e. that binds two HA chains). AIM: Crosslinking by polyethylene glycol diglycidyl ether (PEGDE) has been more recently introduced and showed attractive features in terms of viscoelastic properties and reduced biodegradation. Aim of this paper is to define a method for the determination of the crosslinking properties of these recently introduced fillers, method that is lacking at the moment. MATERIAL AND METHOD: The percentage of crosslinker and the fraction that is effectively crosslinked were determined by proton Nuclear Magnetic Resonance (1H NMR) and by 13C NMR, respectively. The filler were preliminarily washed with acetonitrile to remove residual PEG and then digested by hyaluronidase to obtain a sample that can be analysed by NMR. RESULTS: The crosslinking parameters were determined in four samples of NEAUVIA PEG-crosslinked dermal fillers (produced by MatexLab S.p.A., Italy). The percentage of crosslinker was between 2.8% and 6.2% of HA, whereas the effective crosslinker ratios were between 0.07 and 0.16 (ratio between the moles of effectively crosslinked PEG and total moles of PEG). Moreover, a digestion procedure alternative to enzymatic digestion, based on acidic hydrolysis, was successfully tested for the determination of crosslinker percentage. CONCLUSIONS: The proposed method successfully determined the two crosslinking parameters in PEG-crosslinked dermal fillers. The estimated percentage of crosslinker is similar to previously reported data for other crosslinkers, whereas the effective crosslinker ratio is lower for PEG crosslinked hydrogels.

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In Vitro Evaluation of the Biosafety of Hyaluronic Acid PEG Cross-Linked with Micromolecules of Calcium Hydroxyapatite in Low Concentration

Cited by 26 publications

References 13 publications

Chemical and mechanical characterization of hyaluronic acid hydrogel cross‐linked with polyethylen glycol and its use in dermatology

Chemical and mechanical characterization of hyaluronic acid hydrogel cross‐linked with polyethylen glycol and its use in dermatology

Immune profile of hyaluronic acid hydrogel polyethylene glycol crosslinked: An in vitro evaluation in human polymorphonuclear leukocytes

Chemical Characterization of Hydrogels Crosslinked with Polyethylene Glycol for Soft Tissue Augmentation

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