In vitro evaluation of the antibiofilm properties of chlorhexidine and delmopinol on dental implant surfaces

Ready, Derren; Theodoridis, Georgios; Green, Ingrid; Ciric, Lena; Pratten, Jonathan; Tay, W.M.; McDonald, Alison D.

doi:10.1016/j.ijantimicag.2015.01.020

Cited by 13 publications

(14 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that, with the additional use of 2% chlorhexidine, more anaerobic bacteria on the implant surface were reduced than using mechanical debridement alone. In fact, 2% chlorhexidine was shown to be the most effective concentration previously, achieving a total viable biofilm reduction ranging from 96.2% to more than 99.99%, depending on the time of exposure and the stage of biofilm development [128]. It was also reported that an oral irrigator combined with 0.2% chlorhexidine is effective in reducing biofilms attached to rough titanium surfaces immediately after cleaning [129].…”

Section: Chlorhexidinementioning

confidence: 98%

Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors

Han

Tsoi

Rodrigues

et al. 2016

International Journal of Adhesion and Adhesives

View full text Add to dashboard Cite

a b s t r a c tBacterial adhesion on dental implants may cause peri-implant disease including peri-implant mucositis and peri-implantitis. Peri-implantitis may lead to bone resorption and eventually loss of the implant. Therefore, the factors which influence bacterial adhesion are critical and revealed by many studies. The purpose of this review is to summarize the current knowledge of factors influencing the bacteria adhesion, including local factors of implant surface topography, abutment, cement and oral environment factors of saliva and protein. In addition, the corresponding strategies of surface modifications, coatings and challenges for implant materials as prevention and treatment approaches for bacteria adhesion on implant will also be discussed. We expect to give an overall picture of the bacteria adhesion on implant, and provide future perspectives, such as laser therapy, photocatalysis, plasma and bioelectric effect to inspire researchers to explore on this issue.

show abstract

Section: Chlorhexidinementioning

confidence: 98%

Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors

Han

Tsoi

Rodrigues

et al. 2016

International Journal of Adhesion and Adhesives

View full text Add to dashboard Cite

show abstract

“…Previous in vitro research on implant surfaces has shown that roughness, surface free energy, wettability, and degree of sterilization may affect biofilm formation, bacterial three-dimensional distribution, and antimicrobial treatment efficacy (Al-Ahmad et al, 2010;Di Giulio et al, 2016;Lin, Liu, Wismeijer, Crielaard, & Deng, 2013;Schmidlin et al, 2013;Song et al, 2015;Yeo, Kim, Lim, & Han, 2012). These studies have used specimens, such as disks or slabs containing the studied surface (Aguayo, Donos, Spratt, & Bozec, 2015;de Avila et al, 2015;Di Giulio et al, 2016;Papavasileiou, Behr, Gosau, Gerlach, & Buergers, 2015;Pita et al, 2015;Ready et al, 2015), but these specimens lack the macro-structural and topographic characteristics of the dental implants used clinical practice. Furthermore, many of these in vitro investigations assessing bacterial adhesion and colonization on implant surfaces have used simple biofilm models, consisting of one or, maximum, two bacterial species, and/ or have used short-term evaluations (24 hr or less), thus, lacking the ability to properly study the dynamics of the biofilm maturation and its potential pathogenicity (Pereira et al, 2015;Schmidt et al, 2017;Sridhar et al, 2016).…”

mentioning

confidence: 99%

Topographic characterization of multispecies biofilms growing on dental implant surfaces: An in vitro model

Bermejo

Sanchez

Llama‐Palacios

et al. 2019

Clinical Oral Implants Res

View full text Add to dashboard Cite

Objectives To describe the early biofilm formation over whole dental implants with its micro‐ and macrostructure, using an in vitro multispecies biofilm model. Material and methods Six bacterial strains (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans) were used to develop in vitro biofilms over whole titanium implants (growth times 12, 24, 48, 72, 96, and 120 hr). The morphology of biofilms was studied by confocal laser scanning microscopy and scanning electron microscopy, and the bacterial dynamics through quantitative polymerase chain reaction. As primary outcome variable, numbers of each species [colony‐forming units per milliliter (CFU/ml)] at different incubation times were compared using the one‐way analysis of variance and post hoc testing with Bonferroni's correction. Furthermore, implants were fixed in methacrylate stents to reproduce the clinical situation and biofilms were developed and analyzed by scanning electron microscopy. Results Bacteria colonized implants in a short period of time. Biofilms reached a mature state at 96 hr, exhibiting different ratios of live/dead cells depending on their location, being the peaks of the threads the areas harboring more live bacteria. The densities of each bacteria fluctuated in time, reaching its maximum at 96 hr. Even though the coefficients of variation were high, percentages were similar to those published previously using implant surface specimens, rather than whole implants. Conclusion Dental implants can be colonized by different bacterial species, developing into a mature and well‐structured biofilm, which depending on the location may exhibit different degree of bacterial vitality.

show abstract

“…The percentages of microbicide solutions used in our experiments corresponded to the Centers for Disease Control and Prevention recommendations (29) (e.g., 0.1 to 2% CT and 0.05 to 1% CHX) for disinfection of hard surfaces such as SSWs. Similar percentages of microbicide solutions were recently used effectively against biofilms formed by periodontal pathogens (30)(31)(32) and single strains of relevant Gram-positive and Gram-negative pathogens, including A. baumannii (33). Interestingly, A. baumannii biofilm-associated cells have shown resistance to CHX, but effective concentrations used to kill these bacterial cells are comparable to those used in this study (33).…”

Section: Discussionmentioning

confidence: 51%

Microbicides Alter the Expression and Function of RND-Type Efflux Pump AdeABC in Biofilm-Associated Cells of Acinetobacter baumannii Clinical Isolates

Krishnamoorthy

Shah

Lee

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Acinetobacter baumannii is a Gram-negative bacterium that causes nosocomial infections worldwide. This microbe's propensity to form biofilms allows it to persist and to survive on clinical abiotic surfaces for long periods. In fact, A. baumannii biofilm formation and its multidrug-resistant nature severely compromise our capacity to care for patients in hospital environments. In contrast, microbicides such as cetrimide (CT) and chlorhexidine (CHX) play important roles in the prevention and treatment of infections. We assessed the efficacy of CT and CHX, either alone or in combination, in eradicating A. baumannii biofilms formed by clinical isolates, by using stainless steel washers to mimic hard abiotic surfaces found in hospital settings. We demonstrated that increasing amounts of each microbicide, alone or in combination, were able to damage and to reduce the viability of A. baumannii biofilms efficaciously. Interestingly, the adeB gene of the resistance-nodulation-cell division (RND) family is predominantly associated with acquired resistance to antimicrobials in A. baumannii. We showed that CT and CHX adversely modified the expression and function of the RND-type efflux pump AdeABC in biofilm-associated A. baumannii cells. Furthermore, we established that these microbicides decreased the negative charges on A. baumannii cell membranes, causing dysregulation of the efflux pump and leading to cell death. Our findings suggest that CT and CHX, alone or in combination, can be used efficaciously for eradication of A. baumannii from hospital surfaces, in order to reduce infections caused by this nosocomial agent.

show abstract

In vitro evaluation of the antibiofilm properties of chlorhexidine and delmopinol on dental implant surfaces

Cited by 13 publications

References 12 publications

Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors

Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors

Topographic characterization of multispecies biofilms growing on dental implant surfaces: An in vitro model

Microbicides Alter the Expression and Function of RND-Type Efflux Pump AdeABC in Biofilm-Associated Cells of Acinetobacter baumannii Clinical Isolates

Contact Info

Product

Resources

About