In vitro evaluation of graphene oxide nanosheets on immune function

Feito, María José; Vila, M.; Matesanz, María Concepción; Linares, Javier; Gonçalves, Gil; Marques, Paula A.A.P.; Vallet‐Regí, María; Rojo, José María; Portolés, María Teresa

doi:10.1016/j.jcis.2014.07.004

Cited by 65 publications

(47 citation statements)

References 50 publications

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“…Nevertheless, there is currently no reliable approach to determine the adverse immune responses of the human body to foreign implants prior to their in vivo applications. Conventional cultures of immune cells directly with these implants do not capture the dynamic process of the FBR. Various microfluidic platforms have been recently adapted to study immunological events such as inflammation and immunotherapy, but they have rarely been used in screening the FBR to biomaterials and implants.…”

Section: Parameters and Constants Used For Modelingmentioning

confidence: 99%

A Foreign Body Response‐on‐a‐Chip Platform

Sharifi

Htwe

Righi

et al. 2019

Adv Healthcare Materials

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Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR-on-a-chip (FBROC) for modeling the cascade of events during immune cell response to implants. The platform models the native implant microenvironment where the implants are interfaced directly with surrounding tissues, as well as vasculature with circulating immune cells. The study demonstrates that the release of cytokines such as monocyte chemoattractant protein 1 (MCP-1) from the extracellular matrix (ECM)-like hydrogels in the bottom tissue chamber induces trans-endothelial migration of circulating monocytes in the vascular channel toward the hydrogels, thus mimicking implant-induced inflammation. Data using patient-derived peripheral blood mononuclear cells further reveal interpatient differences in FBR, highlighting the potential of this platform for monitoring FBR in a personalized manner. The prototype FBROC platform provides an enabling strategy to interrogate FBR on various implants, including biomaterials and engineered tissue constructs, in a physiologically relevant and individual-specific manner.

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Section: Parameters and Constants Used For Modelingmentioning

confidence: 99%

A Foreign Body Response‐on‐a‐Chip Platform

Sharifi

Htwe

Righi

et al. 2019

Adv Healthcare Materials

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“…Spleen cells were isolated according to a reported protocol [15], and 5 3 10 6 cells in 1 ml of RPMI medium were activated with the T cell mitogen, ConA (at 2 mg/ml; Sigma-Aldrich, St. Louis, MO, USA) which has been broadly used to analyze cytokine production by spleen cells [16,17]. Culture media were collected 48 h later, and the levels of IL-10, IL-17, and TNF-a determined in duplicate serial dilutions, with ELISA protocols, used as stipulated by the manufacturer (R&D Systems, Minneapolis, MN, USA).…”

Section: Cytokine Productionmentioning

confidence: 99%

The Src kinase Fyn is protective in acute chemical-induced colitis and promotes recovery from disease

Lopes

Wang

Smyth

et al. 2015

Journal of Leukocyte Biology

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Despite progress in understanding enteric inflammation, current therapies, although effective in many patients with inflammatory bowel disease (IBD), have significant sideeffects, and, in many patients, it is refractory to treatment. The Src kinase Fyn mediated IFN-g-induced increased permeability in model epithelia, and so we hypothesized that inhibition of Fyn kinase would be anti-colitic. Mice [B6.129SF2/J wild-type (WT), Fyn KO, or chimeras] received 2.5% dextran sodium sulfate (DSS) or normal water for 10 d and were necropsied immediately or 3 d later. Gut permeability was assessed by FITC-dextran flux, colitis by macroscopic and histologic parameters, and immune cell status by cytokine production and CD4 + T cell Foxp3 expression. Fyn KO mice consistently displayed significantly worse DSS-induced disease than WT, correlating with decreased IL-10 and increased IL-17 in splenocytes and the gut; Fyn KO mice failed to thrive after removal of the DSS water. Analysis of chimeric mice indicated that the increased sensitivity to DSS was due to the lack of Fyn kinase in hematopoietic, but not stromal, cells, in accordance with Fyn + T cell increases in WT mice exposed to DSS and Fyn KO mice having a reduced number of CD4 + Foxp3 + cells in baseline or colitic conditions and a reduced capacity to induce Foxp3 expression in vitro. Other experiments suggest that the colonic microbiota in Fyn KO mice is not preferentially colitogenic. Contrary to our expectation, the absence of Fyn kinase resulted in greater DSS-induced disease, and analysis of chimeric mice indicated that leukocyte Fyn kinase is beneficial in limiting colitis.Abbreviations: CD = cluster of differentiation, = DAS = disease activity score, DSS = dextran sodium sulfate, IBD = inflammatory bowel disease, IDO1 = indoleamine 2,3-deoxygenase-1, KO = knockout, MPO = myeloperoxidase, qRT-PCR = real-time polymerase chain reaction, SAP = SLAM associated peptide, SLAM = signaling through the lymphocyte activation molecule, Treg = regulatory T cell, WT = wild-type 0741-5400/15/0097-1089

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“…Also, it has been known since antiquity that silver nanoparticles (and salts) are highly toxic to microbial organisms [11], and recent investigations into GO have indicated that the pristine material can inhibit the growth of bacterial colonies [12]. Not least, we emphasize that GO may promote increased cell adhesion and even contribute to paracrine effects [13]. As a result, it is reasonable to assume that a combination of the two into a GO-nanoparticle composite could create a highly effective, yet ultrathin, antimicrobial coating that can subsequently be applied to any given surface and promote increased surface adhesion.…”

Section: Introductionmentioning

confidence: 72%

Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

Carreño

Barbosa

Duarte

et al. 2017

Journal of Nanomaterials

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Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positiveStaphylococcus aureus(S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness againstStaphylococcus aureuswas limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity.

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In vitro evaluation of graphene oxide nanosheets on immune function

Cited by 65 publications

References 50 publications

A Foreign Body Response‐on‐a‐Chip Platform

A Foreign Body Response‐on‐a‐Chip Platform

The Src kinase Fyn is protective in acute chemical-induced colitis and promotes recovery from disease

Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

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