In-vitro Evaluation of Antifungal Activity of Ganoderma lucidum Against the Biofilm Producing Candida species

Abstract

Background: Bioproducts of mushrooms Ganoderma lucidum have multi beneficial effects for human welfare. Objectives: The aim of the present study was to evaluate antifungal activity of Ganoderma lucidum against the biofilm producing fungi. In this current study it carries 100 Candida species which forms biofilms were collected from the Immunocompromised and Immuno Suppressive patients. Methods: The fruit bodies of Ganoderma lucidum were collected and done the extraction at various concentrations with the help of Dimethyl sulfoxide (DMSO) and Methanol. The various concentrations of the extracts were applied by Proteinase and phospholipase enzyme secretion assay, Minimum Inhibitory Concentration (MIC) -XTT Assay and Minimal Fungicidal Concentration (MFC). Results: Our results showed the MIC and MFC of DMSO extract were shows highest significant value (P<0.05) against the antifungal activity than the methanolic extract of the Ganoderma lucidum among the various biofilm producing candida species. Conclusion: So we can be conclude that DMSO extract of the Ganoderma lucidum shows high significant factor against the various disease causing Candida species and its biofilm.