In vitro evaluation of Ag doped hydroxyapatite coatings in acellular media

Vrânceanu, Diana Maria; Parau, Anca Constantina; Cotruț, Cosmin M.; Kiss, A.; Constantin, Lidia R.; Braic, V.; Vlădescu, Alina

doi:10.1016/j.ceramint.2019.02.191

Cited by 37 publications

(29 citation statements)

References 68 publications

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“…Also, we showed that SiC-added HAp coating has a superior electrochemical behavior in artificial saliva, being a good solution for dental applications. Moreover, we showed that Ag addition into HAp coating increases its antibacterial abilities without affecting its bioactivity or biomineralization abilities [22].…”

Section: Introductionmentioning

confidence: 94%

“…The biofunctionality and in vitro abilities of HAp can be adjusted by controlling its phase, structure, composition, and morphology. One way to achieve this is by incorporating/adding different elements such as F [11], Mg [12][13][14][15][16][17], Zn [12,[17][18][19][20][21], Ag [16,19,22], Sr [13,17,23], and Si [24][25][26][27][28][29] into the HAp structure, which is very flexible. A considerable number of scientific papers related to the substitution of HAp with various elements can be found in the literature, indicating that this is an important path in the biomaterial and medical fields.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Activity Assays of Sputtered HAp Coatings with SiC Addition in Various Simulated Biological Fluids

et al. 2019

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Considering the requirements of medical implantable devices, it is pointed out that biomaterials should play a more sophisticated, longer-term role in the customization and optimization of the material–tissue interface in order to ensure the best long-term clinical outcomes. The aim of this contribution was to assess the performance of silicon carbide–hydroxyapatite in various simulated biological fluids (Dulbecco’s modified Eagle’s medium (DMEM), simulated body fluid (SBF), and phosphate buffer solution (PBS)) through immersion assays for 21 days at 37 ± 0.5 °C and to evaluate the electrochemical behavior. The coatings were prepared on Ti6Al4V alloy substrates by magnetron sputtering method using two cathodes made of hydroxyapatite and silicon carbide (SiC). After immersion assays the coating’s surface was analyzed in terms of morphology, chemical and phase composition, and chemical bonds. According to the electrochemical behavior in the media investigated at 37 ± 0.5 °C, SiC addition inhibits the dissolution of the hydroxyapatite in DMEM acellular media. Furthermore, after adding SiC, the slow degradation of hydroxyapatite in PBS and SBF media as well as biomineralization in DMEM were observed.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

In Vitro Activity Assays of Sputtered HAp Coatings with SiC Addition in Various Simulated Biological Fluids

et al. 2019

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“…After 21 days of immersion in the FBS supplemented McCoy's 5A solution, the surface of the samples (Figure 7f,h) closely resembled the one observed in the case of films tested for 7 days in SBF (Figure 7a,c), further suggesting that biomineralization processes are slower in the FBS supplemented McCoy's 5A solution with respect to the ones occurring in SBF. Kindred observations were recently reported by Popa et al [59], Vladescu et al [11] and Vranceanu et al [73] which showed that the (i) biomineralization processes (i.e., formation and structuring of biomimetic HA layers) takes place at a lower rate [59] and (ii) the degradation is significantly reduced [11,73] in media with a complex organic-inorganic composition (such as in the FBS-supplemented McCoy's 5A solution) with respect to those encountered in the inorganic SBF solution.…”

Section: In Vitro Tests In Simulated Physiological Solutionsmentioning

confidence: 71%

Animal Origin Bioactive Hydroxyapatite Thin Films Synthesized by RF-Magnetron Sputtering on 3D Printed Cranial Implants

Chioibasu

Duta

Popescu-Pelin

et al. 2019

Metals

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Ti6Al4V cranial prostheses in the form of patterned meshes were 3D printed by selective laser melting in an argon environment; using a CO2 laser source and micron-sized Ti6Al4V powder as the starting material. The size and shape of prostheses were chosen based on actual computer tomography images of patient skull fractures supplied in the framework of a collaboration with a neurosurgery clinic. After optimizations of scanning speed and laser parameters, the printed material was defect-free (as shown by metallographic analyses) and chemically homogeneous, without elemental segregation or depletion. The prostheses were coated by radio-frequency magnetron sputtering (RF-MS) with a bioactive thin layer of hydroxyapatite using a bioceramic powder derived from biogenic resources (Bio-HA). Initially amorphous, the films were converted to fully-crystalline form by applying a post-deposition thermal-treatment at 500 °C/1 h in air. The X-ray diffraction structural investigations indicated the phase purity of the deposited films composed solely of a hexagonal hydroxyapatite-like compound. On the other hand, the Fourier transform infrared spectroscopic investigations revealed that the biological carbonatation of the bone mineral phase was well-replicated in the case of crystallized Bio-HA RF-MS implant coatings. The in vitro acellular assays, performed in both the fully inorganic Kokubo’s simulated body fluid and the biomimetic organic–inorganic McCoy’s 5A cell culture medium up to 21 days, emphasized both the good resistance to degradation and the biomineralization capacity of the films. Further in vitro tests conducted in SaOs-2 osteoblast-like cells showed a positive proliferation rate on the Bio-HA RF-MS coating along with a good adhesion developed on the biomaterial surface by elongated membrane protrusions.

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“…Researchers around the world have focused on developing various surface engineering tools to obtain tunable surface properties of the implants. Of the methods used for this purpose, the following can be found machining/ micromachining, airborne-particle abrasion, acid etching, electropolishing, anodic oxidation, electrochemical deposition, pulsed laser deposition, chemical and physical vapor deposition, and plasma spraying [15][16][17].…”

Section: Techniques Used To Increase Apatite Formationmentioning

confidence: 99%

Materials and manufacturing techniques trends in prosthetic dentistry.

Cotruț

2022

Acta Stomatologica Marisiensis Journal

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In vitro evaluation of Ag doped hydroxyapatite coatings in acellular media

Cited by 37 publications

References 68 publications

In Vitro Activity Assays of Sputtered HAp Coatings with SiC Addition in Various Simulated Biological Fluids

In Vitro Activity Assays of Sputtered HAp Coatings with SiC Addition in Various Simulated Biological Fluids

Animal Origin Bioactive Hydroxyapatite Thin Films Synthesized by RF-Magnetron Sputtering on 3D Printed Cranial Implants

Materials and manufacturing techniques trends in prosthetic dentistry.

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