In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca

Grochowski, D; Uysal, Şengül; Aktümsek, Abdurrahman; Granica, Sebastian; Ceylan, Ramazan; Locatelli, Marcello; Tomczyk, Michał

doi:10.1016/j.phytol.2017.03.005

Cited by 266 publications

(189 citation statements)

References 30 publications

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“…Recently, the use of AChE inhibitors has been shown to be an effective clinical approach to maintain the levels of acetylcholine and enhance cholinergic function. The inhibition of the AChE and BChE has become a standard approach in the symptomatic treatment of neurodegenerative diseases such as Alzheimer's disease . Also, α‐amylase and α‐glucosidase are critical players in the control of glycaemic level.…”

Section: Resultsmentioning

confidence: 99%

Chemical profile, antioxidant, and enzyme inhibitory properties of two Scutellaria species: S. orientalis L. and S. salviifolia Benth

Zengin

Llorent‐Martínez

Molina-García

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives This study investigates into the biological effects of solvent extracts (ethyl acetate, methanol, and water) of Scutellaria orientalis L. and Scutellaria salviifolia Benth. based on its enzyme inhibitory activity and antioxidant ability together with the screening of bioactive compounds. Methods Total and individual bioactive components were determined using spectrophotometric and HPLC‐ESI‐MS methods. Six antioxidant assays were conducted and enzyme inhibition was tested against key enzymes linked to the pathology of common chronic disorders. Key findings Results revealed that the aqueous extracts of both plants exerted better 2,2′‐azino‐bis(3‐ethylbenzothiazoline)‐6‐sulfonic acid scavenging, reducing power, metal chelating, and α‐glucosidase inhibitory activities. The methanol extracts showed highest tyrosinase inhibition and antioxidant activity in phosphomolybdenum assay while the less polar ethyl acetate extracts showed better acetylcholinesterase, butyrylcholinesterase, and α‐amylase inhibition. Phytochemical evaluation by HPLC‐ESI‐MS revealed the presence of high amounts of flavones. Conclusions Scutellaria orientalis and S. salviifolia are important sources of bioactive agents that warrants further studies.

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Section: Resultsmentioning

confidence: 99%

Chemical profile, antioxidant, and enzyme inhibitory properties of two Scutellaria species: S. orientalis L. and S. salviifolia Benth

Zengin

Llorent‐Martínez

Molina-García

et al. 2018

Journal of Pharmacy and Pharmacology

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“…The enzyme inhibitory activity was detected against a panel of important enzymes such as cholinesterases (AChE and BChE), tyrosinase, lipase, α‐amylase and α‐glucosidase using the methods as described previously . The enzyme inhibitory actions of extracts were assessed as equivalents of kojic acid (KAE) for tyrosinase, galantamine for AChE and BChE, orlistat (Xenical®) for lipase, and acarbose for α‐amylase and α‐glucosidase.…”

Section: Methodsmentioning

confidence: 99%

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

et al. 2019

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BACKGROUND In this study, we aimed to evaluate the influence of different extraction procedures [decoction, homogenizer‐assisted extraction (HAE), infusion, maceration, Soxhlet and ultrasound‐assisted extraction (UAE)] on the chemical profiling and biological properties of methanol and water extracts of Pulicaria dysenterica (L.) Bernh. The chemical profiles of the extracts were evaluated by high‐performance liquid chromatography coupled to electrospray ionization and time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS). The antioxidant properties and enzymes (lipase, α‐amylase, α‐glucosidase, tyrosinase and cholinesterases) inhibitory potential of the extracts were evaluated. RESULTS The chemical profiles were dependent on the type of extraction methods as well as on the type of solvent. The methanolic extracts showed higher levels of total phenolic, flavonoid, and phenolic acid content, while the highest total flavonol content was observed in the HAE–water extract. Forty different compounds were identified from P. dysenterica. In relation to the potential in vitro anti‐diabetic effects, the highest activity against the studied key enzymes was observed for the macerated extract (α‐amylase: 0.58 ± 0.03 and α‐ glucosidase: 1.65 ± 0.03 mmol ACAE g−1). The HAE–methanol extract was the most potent inhibitor of cholisterases, whereas the highest activities against tyrosinase were observed for UAE–methanol extract, followed by macerated and Soxhlet. The inhibitory activity of the studied extracts against lipase were in the order: soxhlet > macerated> HAE–methanol > UAE–methanol. CONCLUSION This study has established scientific baseline data on the therapeutic properties of P. dysentrica, thereby advocating the need for further investigations in an endeavour to develop novel pharmaceuticals from this plant. © 2019 Society of Chemical Industry

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“…The antioxidant potential of the samples was tested using a series of assays which include phosphomolybdenum total antioxidant, antiradical [DPPH and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid (ABTS)], reducing power [ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC)], and also ferrous chelating assays following the methods published by Grochowski et al . Trolox equivalents were used for expression of antioxidant activities.…”

Section: Methodsmentioning

confidence: 99%

Chemical fingerprint and bioactivity evaluation of Globularia orientalis L. and Globularia trichosantha Fisch. & C. A. Mey. using non‐targeted HPLC‐ESI‐QTOF‐MS approach

Rodríguez‐Pérez

Zengin

Segura‐Carretero

et al. 2018

Phytochemical Analysis

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Introduction In the quest for new sources of biologically‐active compounds, the chemical, and biological profiles of two Globularia species (G. trichosantha Fisch. & C. A. Mey and G. orientalis L.) were investigated. Methodology Chemical profiles were evaluated by high‐performance liquid chromatography coupled to electrospray ionisation and quadrupole time‐of‐flight mass spectrometry (HPLC‐ESI‐QTOF‐MS), as well as by their total phenolic, flavonoids, and phenolic acids contents. The antioxidant abilities of the investigated extracts were done using different assays including free radical scavenging [1,1‐diphenyl‐2‐picryl‐hydrazyl (DPPH) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid (ABTS)], reducing power (cupric reducing antioxidant capacity and ferric reducing antioxidant power), phosphomolybdenum, and metal chelating. Inhibitory potential against key enzymes involved in neurodegenerative diseases (cholinesterases; AChE, and BChE), diabetes (α‐glucosidase and α‐amylase), hyperpigmentation (tyrosinase) and obesity (pancreatic lipase) were evaluated. Results Globularia trichosantha and G. orientalis extracts showed remarkable antioxidant properties, with the water extracts being a better source of antioxidant compounds. Both species showed remarkable inhibitory effects against the target enzymes. However, for both species, only the acetyl acetate and methanolic extracts were potent against cholinesterases and lipase. Conclusion HPLC‐ESI‐QTOF‐MS analysis revealed the presence of 107 compounds from G. trichosantha and G. orientalis, among which, 43 compounds have been preliminarily characterised for the first time from the Globulariaceae family. To date, this study can be considered as the most comprehensive research focused on the characterisation of G. trichosantha and G. orientalis. Results amassed from this study tend to show that these plants represent a rich source of biologically active compounds which can be further explored and validated for their therapeutic potential.

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In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca

Cited by 266 publications

References 30 publications

Chemical profile, antioxidant, and enzyme inhibitory properties of two Scutellaria species: S. orientalis L. and S. salviifolia Benth

Chemical profile, antioxidant, and enzyme inhibitory properties of two Scutellaria species: S. orientalis L. and S. salviifolia Benth

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

Chemical fingerprint and bioactivity evaluation of Globularia orientalis L. and Globularia trichosantha Fisch. & C. A. Mey. using non‐targeted HPLC‐ESI‐QTOF‐MS approach

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