In vitro digestion of lactoferrin-glycomacropeptide nanohydrogels incorporating bioactive compounds: Effect of a chitosan coating

Bourbon, Ana I.; Pinheiro, Ana Cristina; Cerqueira, Miguel A.

doi:10.1016/j.foodhyd.2018.06.015

Cited by 25 publications

(18 citation statements)

References 26 publications

(38 reference statements)

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“…5A). Bourbon et al (2018) observed a similar behaviour when using a dynamic in vitro GI model to determine the bioavailable fraction of caffeine (as hydrophilic model compound) using Lf-GMP nanohydrogels with chitosan coating as the encapsulation matrix. The authors obtained a caffeine bioavailability of 63.1 ± 0.2 % when caffeine was associated to nanohydrogels and of 59.2 ± 2.1 % when caffeine is present in its free form.…”

Section: Bioactive Model Compounds Bioaccessibility and Bioavailabilitymentioning

confidence: 59%

“…Generally, food proteins are usually hydrolysed in the stomach environment (Zhang & Vardhanabhuti, 2014). Bourbon et al (2018) evaluated both lactoferrin (Lf) and glycomacropeptide (GMP) nanohydrogels hydrolysis using an in vitro dynamic GI model. These authors observed that at least 45 % of Lf and 70 % of GMP remain undigested after simulated gastric phase.…”

Section: β-Lg Micro-and Nanostructures Hydrolysis Degree During In VImentioning

confidence: 99%

“…The steady interest on the improvement of food quality and human health led to a growing use of bioactive compounds level in food products (Chen, Zou, Liu, & McClements, 2016). Commonly, challenges regarding to their inclusion in food products are attributed to the poor solubility (e.g., hydrophobic compounds) in food matrices and their instability along the digestion process (Bourbon, Pinheiro, Cerqueira, & Vicente, 2018). Thus, to overcome these factors, designing of an appropriate bio-based system to transport and deliver bioactive compounds to a specific target site is of utmost importance (Gonçalves, Martins, Duarte, Vicente, & Pinheiro, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

Simões

Martins

Pinheiro

et al. 2020

Food Research International

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Lactoglobulin (β-Lg) is known to be capable to bind hydrophilic and hydrophobic bioactive compounds. This research aimed to assess the in vitro performance of β-Lg micro-(diameter ranging from 200 to 300 nm) and nano (diameter < 100 nm) structures associated to hydrophilic and hydrophobic model compounds on Caco-2 cells and under simulated gastrointestinal (GI) conditions. Riboflavin and quercetin were studied as hydrophilic and hydrophobic model compounds, respectively. Cytotoxicity experiment was conducted using in vitro cellular model based on human colon carcinoma Caco-2 cells. Moreover, the digestion process was simulated using the harmonized INFOGEST in vitro digestion model, where samples were taken at each phase of digestion processoral, gastric and intestinal-and characterized in terms of particle size, polydispersity index (PDI), surface charge by dynamic light scattering (DLS); protein hydrolysis degree by 2,4,6-trinitrobenzene sulfonic acid (TNBSA) assay and native polyacrylamide gel electrophoresis; and bioactive compound concentration. Caco-2 cell viability was not affected up to 21 × 10 −3 mg mL −1 of riboflavin and 16 × 10 −3 mg mL −1 quercetin on β-Lg micro-and nanostructures. In the oral phase, β-Lg structures' particle size, PDI and surface charge values were not changed comparing to the initial β-Lg structures (i.e., before being subjected to in vitro GI digestion). During gastric digestion, β-Lg structures were resistant to proteolytic enzymes and to acid environment of the stomachconfirmed by TNBSA and native gel electrophoresis. In vitro digestion results indicated that β-Lg micro-and nanostructures protected both hydrophilic and hydrophobic compounds from gastric conditions and deliver them to target site (i.e., intestinal phase). In addition, β-Lg structures were capable to enhance riboflavin and quercetin bioaccessibility and bioavailability potential compared to bioactive compounds in their free form. This study indicated that β-Lg micro-and nanostructures were capable to enhance hydrophilic and hydrophobic compounds bioavailability potential and they can be used as oral delivery systems.

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Section: Bioactive Model Compounds Bioaccessibility and Bioavailabilitymentioning

confidence: 59%

Section: β-Lg Micro-and Nanostructures Hydrolysis Degree During In VImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

Simões

Martins

Pinheiro

et al. 2020

Food Research International

Self Cite

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“…These authors concluded that the chitosan layer improved the stability and bioaccessibility of active compounds during in vitro digestion. Furthermore, the degradation of the lipophilic compound was lower when compared with the system without the chitosan layer [99].…”

Section: Controlled Releasementioning

confidence: 99%

Protein-Based Nanostructures for Food Applications

Bourbon

Pereira

Pastrana

et al. 2019

Gels

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Proteins are receiving significant attention for the production of structures for the encapsulation of active compounds, aimed at their use in food products. Proteins are one of the most used biomaterials in the food industry due to their nutritional value, non-toxicity, biodegradability, and ability to create new textures, in particular, their ability to form gel particles that can go from macro- to nanoscale. This review points out the different techniques to obtain protein-based nanostructures and their use to encapsulate and release bioactive compounds, while also presenting some examples of food grade proteins, the mechanism of formation of the nanostructures, and the behavior under different conditions, such as in the gastrointestinal tract.

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“…Some nutraceuticals, such as silymarin, curcumin, vitamin E, docosahexaenoic acid, choline and phosphatidylcholine are used in treating and preventing steatosis [15]. Additionally, many nutraceutical products, such as gallic acid, caffeine, curcumin and others act as anti-aging and antioxidant agents [16, 17]. PUFA-rich fish oils reduce the risk of coronary cardiovascular diseases and enhance the brain functions [18].…”

Section: Introductionmentioning

confidence: 99%

Nutraceuticals’ Novel Formulations: The Good, the Bad, the Unknown and Patents Involved

Helal

Eassa

Amer³

et al. 2019

DDF

105

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: Traditional nutraceuticals and cosmeceuticals hold pragmatic nature with respect to their definitions, claims, purposes and marketing strategies. Their definitions are not well established worldwide. They also have different regulatory definitions and registration regulatory processes in different parts of the world. Global prevalence of nutraceuticals and cosmeceuticals is noticeably high with large market share with minimal regulation compared to traditional drugs. The global market is flooded with nutraceuticals and cosmeceuticals claiming to be of natural origin and sold with a therapeutic claim by major online retail stores such as Amazon and eBay. Apart from the traditional formulations, many manufacturers and researchers use novel formulation technologies in nutraceutical and cosmeceutical formulations for different reasons and objectives. Manufacturers tend to differentiate their products with novel formulations to increase market appeal and sales. On the other hand, researchers use novel strategies to enhance nutraceuticals and cosmeceuticals activity and safety. : The objective of this review is to assess the current patents and research adopting novel formulation strategies in nutraceuticals and cosmeceuticals. Patents and research papers investigating nutraceutical and cosmeceutical novel formulations were surveyed for the past 15 years. Various nanosystems and advanced biotechnology systems have been introduced to improve the therapeutic efficacy, safety and market appeal of nutraceuticals and cosmeceuticals, including liposomes, polymeric micelles, quantum dots, nanoparticles, and dendrimers. This review provides an overview of nutraceuticals and cosmeceuticals current technologies, highlighting their pros, cons, misconceptions, regulatory definitions and market. This review also aims in separating the science from fiction in the nutraceuticals and cosmeceuticals development, research and marketing.

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In vitro digestion of lactoferrin-glycomacropeptide nanohydrogels incorporating bioactive compounds: Effect of a chitosan coating

Cited by 25 publications

References 26 publications

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

Protein-Based Nanostructures for Food Applications

Nutraceuticals’ Novel Formulations: The Good, the Bad, the Unknown and Patents Involved

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