In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells

Guan, Lina; Li, Shaoqing; Wang, Yunfang; Yue, Huimin; Liu, Daqing; He, Lijuan; Bai, Cixian; Yan, Fang; Nan, Xue; Shi, Shuangshuang; Pei, Xuetao

doi:10.1007/s11434-006-2055-7

Cited by 16 publications

(9 citation statements)

References 23 publications

(22 reference statements)

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“…Because the formation of a capillary-like network is the specialized function of endothelial cells [27,30,31] , the RCE cell line established here is indeed an endothelial cell line. This conclusion is coincident with that of human vascular endothelial cells differentiated from human adipose tissue derived mesenchymal stem cells reported by Guan et al [43] . Morphology recovery with 5% BCS-containing MEM medium showed that the morphology of the RCE cells recovered from fibroblastic to polygonal on day 7.…”

Section: Discussionsupporting

confidence: 92%

Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus

Fan

Zhao

et al. 2007

SCI CHINA SER C

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To develop a rabbit corneal endothelial (RCE) cell line, in vitro culture of RCE cells was initiated from Oryctolagus curiculus corneas and a novel RCE cell line was established in this study. To initiate the primary culture of RCE cells, corneas from rabbit eyes were sliced and attached into glutin-coated wells with endothelial cell surface down. After being cultured at a time-gradient interval from 48 to 6 h, the corneal slices were detached and reattached into new wells, respectively. Cells in the wells containing only a pure population of RCE cells were collected and cultured in 20% FBS-DMEM/F12 medium containing chondroitin sulfate, ocular extract, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), carboxymethyl-chitosan, N-acetylglucosamine hydrochloride, glucosamine hydrochloride, culture medium of rabbit corneal stromal cells and oxidation-degradation products of chondroitin sulfate at 37 degrees C, 5% CO(2). The cultured RCE cells, in quadrangle and polygonal shapes, proliferated to confluence 3 weeks later. During the subsequent subculture, the shape of RCE cells changed gradually from polygonal to more fibroblastic. A novel RCE cell line, growing at a steady rate, with a population doubling time of 53.8 h, has been established and subcultured to passage 67. Chromosome analysis showed that the RCE cells exhibited chromosomal aneuploidy with the modal chromosome number of 44. The results of immuno-cytochemical staining with neuron specific enolase (NSE) confirmed that the RCE cells were in neuroectodermal origin. Combined with the results of vascular endothelial growth factor (VEGF) treatment and endothelial cell morphology recovery, it can be concluded that the cell line established here is an RCE cell line. This RCE cell line may serve as a useful tool in theoretical researches of mammalian corneal endothelial cells, and may also have potential application in artificial corneal endothelium development.

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Section: Discussionsupporting

confidence: 92%

Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus

Fan

Zhao

et al. 2007

SCI CHINA SER C

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“…6 In the present study, the differentiated ASC-EPCs had increased expression of CD34 and VEGFR2 as well as functional vascular characteristics. Some previous studies 25,26 revealed that human ASCs had a low level of expression of VEGFR2 and could gradually differentiate into endothelial-like cells with increased expression of CD34 and VEGFR2. Therefore, we termed endothelial-differentiated ASCs as ASC-EPCs.…”

Section: Discussionmentioning

confidence: 97%

Differentiation of canine adipose tissue–derived mesenchymal stem cells towards endothelial progenitor cells

Kang¹,

Lee²,

Kweon³

et al. 2014

ajvr

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“…As hAF-MSCs can give rise to osteogenic, chondrogenic, adipogenic, myogenic, endothelial, hepatic or neurogenic lineages (3) they have been shown to be effective in the treatment of many diseases (4). Due to the endothelial differentiation potential of MSCs, they have received a significant amount of attention as an autologous source for cell-based therapy in restoring endothelial functions and promoting vascularization (5,6).…”

Section: Introductionmentioning

confidence: 99%

Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells

et al. 2019

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Human amniotic fluid (hAF) mesenchymal stem cells (MSCs) are commonly cultured in medium containing FBS. However, there are concerns about using animal serum in therapeutic applications due to the potential for immunogenic reactions and the risk of transmission of pathogens. For safety reasons, human platelet lysate (hPL) has been suggested as a replacement for FBS because it appears to be a natural source of growth factors. In this present study, it was investigated whether FBS could be substituted with hPL in hAF-MSCs culture without affecting their properties. Pooled hPL was generated by the freeze-thaw method. The concentration of hPL was selected after evaluation by MTT assay. The hAF-MSCs were cultured in FBS- or hPL-supplemented conditions and shared a fibroblast-like morphology. Cell proliferation assays showed that the growth characteristic of hAF-MSCs cultured in 10% hPL-supplemented media was similar to those cultured in 10% FBS-supplemented media. The expression of MSC markers did not differ between the cells cultured in the different conditions. The endothelial differentiation potential was also investigated. Reverse transcription-quantitative (RT-q)PCR revealed that induced cells supplemented with hPL showed an increase level of endothelial specific gene expression compared to the FBS-supplemented cells. Immunofluorescence analysis showed specific protein localization in both induced cell groups. Additionally, induced cells supplemented with hPL had the potential to form networks on Matrigel. This present study indicated that hPL could be used to culture and enhance the endothelial differentiation potential of hAF-MSCs.

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In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells

Cited by 16 publications

References 23 publications

Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus

Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus

Differentiation of canine adipose tissue–derived mesenchymal stem cells towards endothelial progenitor cells

Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells

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