In vitro Demonstration of Cancer Inhibiting Properties from Stratified Self-Organized Plasma-Liquid Interface

Abstract

Experiments on plasma-liquid interaction and formation of thinly stratified self-organized patterns at plasma-liquid interface have revealed a nontrivial cancer-inhibiting capability of liquid media treated at self-organized interfacial patterns. A pronounced cancer suppressing activity towards at least two cancer cells, breast cancer MDA-MB-231 and human glioblastoma U87 cancer lines, was demonstrated in vitro. After a short treatment at the thinly stratified self-organized plasma-liquid interface pattern, the cancer inhibiting media demonstrate pronounced suppressing and apoptotic activities towards tumor cells. Importantly, this would have been impossible without interfacial stratification of plasma jet to thin (of several µm) current filaments, which plays a pivotal role in building up the cancer inhibition properties. Furthermore, thinly stratified, self-organized interfacial discharge is capable to efficiently control the ROS and RNS concentrations in the cancer-inhibiting media. In particular, abnormal ROS/RNS ratios are not achievable in discharges since they do not form stratified thin-filament patterns. Our findings could be tremendously important for understanding the cancer proliferation problem and hence, the potential of this approach in tackling the challenges of high cancer-induced mortality should be explored.

Self-consistent modeling of self-organized patterns of spots on anodes of DC glow discharges

“…Bombardment on the anode by low-kinetic energy ions occurs inside the spots. An interesting hypothesis is that the ions incident on the anode contribute to or are responsible for the cancer-inhibiting effect reported in [18].…”

Self-organized patterns by a DC pin liquid anode discharge in ambient air: Effect of liquid types on formation

“…• CBLD in noble gas on the cathode [20][21][22][23][24] • DBD with a semiconductor electrode 25 • Glow discharge at at 1 atm on the anode 26,27 and low pressure [28][29][30][31][32] • Pin liquid electrode discharge in the air with auxiliary gas [33][34][35] and without [36][37][38] .…”

Sensitivity of tumor versus normal cell migration and morphology to cold atmospheric plasma‐treated media in varying culture conditions

“…While CAP has already been shown as a promising therapeutic for external wounds and for destroying certain cells while leaving others intact, it remains unclear how CAP would be used in vivo in the presence of bodily fluids with highly variable compositions. To facilitate potential future CAP delivery in vivo , many in vitro studies that assess the effectiveness of CAP as a cancer therapy are conducted in cell‐culture media, water, or buffer solutions . Hence, it is critical to understand what effect various cell‐culture parameters have on the outcome of CAP‐treated media‐based cancer treatment studies.…”