In vitro cytotoxicity of curcuminoids against head and neck cancer HNO97 cell line

Abstract

Oral squamous cell carcinoma (OSCC) is a malignant tumour of Head and Neck Cancer (HNC). The recent therapeutic approaches used to treat cancer have adverse side effects. The natural agents exhibiting anticancer activities are generally considered to have a robust therapeutic potential. Curcuminoids, one of the major active compounds of the turmeric herb, are used as a therapeutic agent for several diseases including cancer. In this study, the cytotoxicity of curcuminoids was investigated against OSCC cell line HNO97. Our data showed that curcuminoids significantly inhibits the proliferation of HNO97 in a time and dose-dependent manner (IC50=35 μM). Cell cycle analysis demonstrated that curcuminoids increased the percentage of G2/M phase cell populations in the treated groups. Treating HNO97 cells with curcuminoids led to cell shrinking and increased detached cells, which are the typical appearance of apoptotic cells. Moreover, flow cytometry analysis revealed that curcuminoids significantly induced apoptosis in a time-dependent manner. Furthermore, as a response to curcuminoids treatment, comet tails were formed in cell nuclei due to the induction of DNA damage. Curcuminoids treatment reduced the colony formation capacity of HNO97 cells and induced morphological changes. Overall, these findings demonstrate that curcuminoids can in vitro inhibit HNC proliferation and metastasis and induce apoptosis.

Complementary and alternative therapies in skin cancer a literature review of biologically active compounds

“…Curcuminoids consist of diferuloylmethane (curcumin), desmethoxycurcumin, and bisdemethoxycurcumin 15 . A study in human oral squamous cell carcinoma (HNO97 cell line) showed inhibition in proliferation, morphological changes (apoptosis‐like), alterations in cell cycle distribution, and DNA damage 15 . Also investigators found apoptosis induction and suppression of colony‐forming ability in a time‐dependent manner 15 …”

“…Those with activity in skin tumors and NMSC are resveratrol, 14 cryptolepine, apigenin, 13 and curcumin (Diferuloylmethane) 15 . Curcumin has been particularly evaluated on SCC.…”

“…Curcuminoids are a spice with a yellow color extracted from the roots of the Curcuma longa herb (Turmeric), which belongs to the ginger family. Curcumin is also known as ‘Golden spice’ because of its yellow color found in curry powder 15 . Curcuminoids consist of diferuloylmethane (curcumin), desmethoxycurcumin, and bisdemethoxycurcumin 15 .…”

“…15 Also investigators found apoptosis induction and suppression of colony-forming ability in a time-dependent manner. 15 Silymarin/Silibinin. Silymarin is isolated from the seed of milk thistle (Silybum marianum, Astteraceae family), which is an annual or biennial Mediterranean native herb.…”

“…Those with activity in skin tumors and NMSC are resveratrol, 14 cryptolepine, apigenin, 13 and curcumin (Diferuloylmethane). 15 Curcumin has been particularly evaluated on SCC. A phase I trial in humans demonstrated that 8000 mg/day curcumin were non-toxic when • Morphological changes of HNO97 cell lines: appearance of apoptosis.…”