In vitro collection and posttransfusion engraftment characteristics of MNCs obtained by using a new separator for autologous PBPC transplantation

Snyder, Edward L.; Baril, Laurene L.; Cooper, Dennis; Min, Kyungyoon; Mechanic, S.; Stoddart, Lanu V.; Burtness, Barbara; Seagraves, P.; Debelak, Jacob P.; Gudino, Maria; McCullough, James L.

doi:10.1046/j.1537-2995.2000.40080961.x

Cited by 39 publications

(60 citation statements)

References 9 publications

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“…11 The more aggressive settings were used by Ikeda et al when comparing the AM to the SP. 15 They found equivalent CD34 þ cell yields, white blood cell content, mononuclear cell content and colony-forming units granulocyte/macrophage (CFU-GM). They found similar collection efficiencies for CD34 þ cells, white blood cells and mononuclear cells but significantly lower collection efficiencies for platelets.…”

Section: Discussionmentioning

confidence: 98%

Apheresis instrument settings influence infused absolute lymphocyte count affecting survival following autologous peripheral hematopoietic stem cell transplantation in non-Hodgkin's lymphoma: the need to optimize instrument setting and define a lymphocyte collection target

Katipamula

Porrata

Gastineau

et al. 2006

Bone Marrow Transplant

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Section: Discussionmentioning

confidence: 98%

Apheresis instrument settings influence infused absolute lymphocyte count affecting survival following autologous peripheral hematopoietic stem cell transplantation in non-Hodgkin's lymphoma: the need to optimize instrument setting and define a lymphocyte collection target

Katipamula

Porrata

Gastineau

et al. 2006

Bone Marrow Transplant

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“…Apheresis devices have progressively improved in recent years and newly introduced cell separators show an improved collection eciency (CE), a higher purity of MNC, and lower platelets contamination [6,7]. A study comparing six dierent cell separators showed that MNC CE varied from 38 to 71% [1] and a study conducted in a pediatric setting comparing three dierent devices showed that CD34+ cells CE ranged from 42 to 51% [4].…”

Section: Discussionmentioning

confidence: 99%

Mononuclear cell collection in patients undergoing extra‐corporeal photo‐chemotherapy for acute and chronic graft‐vs.‐host‐disease (GvHD): Comparison between COBE Spectra version 4.7 and 6.0 (AutoPBSC)

Perseghin

Dassi

Balduzzi

et al. 2002

J of Clinical Apheresis

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A constant improvement in the performance of blood cell separators has been observed in recent years, allowing better yields in peripheral blood stem cell collection (PBSC) either from healthy donors or for autologous purposes. Nevertheless, to our knowledge, no reports on the efficiency of mononuclear cell (MNC) collection in patients undergoing extra-corporeal photochemotherapy (ECP) for graft-vs.-host-disease (GvHD) have been published. We retrospectively investigated the efficiency of 167 MNC collections performed consecutively in 12 patients between January 1999 and June 2001 by means of the COBE Spectra version 4.7 (V 4.7) or version 6.0 (V 6.0), for 109 and 58 procedures, respectively. MNC fractional extraction (FE) was higher in the V 6.0 group compared to the V 4.7 group : 0.59 +/- 0.21 vs. 0.51 +/- 0.22 (P < 0.05). However, platelet contamination was lower in the products obtained with V.6.0 compared to those obtained with V.4.7: 740 ( 630 x 10(3)/(L vs. 2,073 ( 1,429 x 10(3)/(L (P < 0.05). Only two patients with acute GvHD, both from V 4.7 group, required post-ECP platelet transfusion. The recently released version 6.0 allowed a satisfactory MNC yield with minimal platelet contamination in patients scheduled to undergo ECP for acute or chronic GvHD.

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“…Snyder et al showed a very good performance of the system in leukaphereses of mobilized and nonmobilized adult patients and donors [2,3]. With extended use of this system, its characteristics were described in more detail.…”

Section: Introductionmentioning

confidence: 99%

Performance of a new separator system for routine autologous hematopoietic progenitor cell collection in small children

Witt

Beiglböck

Ritter

et al. 2007

J of Clinical Apheresis

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The AMICUS system was recently introduced for peripheral blood stem cell (PBSC) aphereses in adults. We conducted a single center field evaluation to obtain data about the performance of this system in children with a body weight (bw) < 25 kg. Results of blood priming procedures were compared to historical data obtained with the Fenwal CS3000+ (CS 3000). From August, 2001 to February, 2007, 47/178 (26%) PBSC aphereses procedures were performed in our institution with the AMICUS system in 35 small patients (median bw 13.9 kg; range 6.7-24; age 2.78 years; range 0.97-7.06). The patients suffered from various malignant primary diseases or recurrences. We primed the system with packed RBC in case of >30% dilution of the RBC volume (n = 31) or with saline (n = 16). Compared to the CS3000, the AMICUS revealed comparable collection efficiencies (CE) for CD34+ cells (median 67%, range 26-120), lymphocytes (75%, 25-138), monocytes (54%, 23-173), and granulocytes (10%, 1.5-36), MNC (57% 24-125), but a significantly higher erythrocyte and granulocyte, and a lower platelet CE. There was a significant negative correlation between total leukocyte count and CE for MNC (r = -0.566; P < 0.001) and CD34+ cells (r = -0.517; P < 0.001). There was no significant statistical or clinical difference between the CE in blood-primed procedures and saline-primed procedures. With the AMICUS we saw statistically less citrate reactions compared to the CS 3000. We conclude that the AMICUS system is safe and efficient to harvest PBSC on a routine basis in pediatric patients, even in children < or =10 kg bw.

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In vitro collection and posttransfusion engraftment characteristics of MNCs obtained by using a new separator for autologous PBPC transplantation

Cited by 39 publications

References 9 publications

Apheresis instrument settings influence infused absolute lymphocyte count affecting survival following autologous peripheral hematopoietic stem cell transplantation in non-Hodgkin's lymphoma: the need to optimize instrument setting and define a lymphocyte collection target

Apheresis instrument settings influence infused absolute lymphocyte count affecting survival following autologous peripheral hematopoietic stem cell transplantation in non-Hodgkin's lymphoma: the need to optimize instrument setting and define a lymphocyte collection target

Mononuclear cell collection in patients undergoing extra‐corporeal photo‐chemotherapy for acute and chronic graft‐vs.‐host‐disease (GvHD): Comparison between COBE Spectra version 4.7 and 6.0 (AutoPBSC)

Performance of a new separator system for routine autologous hematopoietic progenitor cell collection in small children

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