In-vitro characterization and cytotoxicity study of flutamide loaded cyclodextrin nanosponges

Allahyari, Saeideh; Esmailnezhad, Neda; Valizadeh, Hadi; Ghorbani, Marjan; Jelvehgari, Mitra; Ghazi, Farhood; Zakeri–Milani, Parvin

doi:10.1016/j.jddst.2020.102275

Cited by 24 publications

(34 citation statements)

References 21 publications

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“…PMDA and CDI nanosponges showed encapsulation efficiency of 91 and 92% for Nisin Z, respectively, but also they revealed significant drug loading capacity, as previous studies reported the high encapsulation efficiency of CD-NSs for various drugs [ 33 , 49 , 50 ]. This result was in accordance with the outcomes of other studies that revealed the high encapsulation efficiency of resveratrol, oxyresveratrol, bovine serum albumin (BSA), and insulin complexed by different nanosponges [ 42 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 93%

“…The specific structure of β-CD-NS led to their consideration as a prominent carrier [ 30 ]. Particularly, in addition to the biocompatibility typical of polysaccharides, they can interact with biological tissues via their tunable functional groups, which makes CD-NSs a good tool for targeted drug delivery [ 33 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…The cellular uptake outcomes illustrated the Coumarin-6 were internalized into the cancer cells effectively, suggesting the uptake of Nisin too. Previous studies indicated that the nanosponges as drug carriers can effectively deliver the biological agents inside the cancer cells [ 33 , 55 ]. The results of MTT and LDH assay illustrated that the cell cytotoxicity and membrane damage in both HT-29 and MCF-7 cancer cells depended on concentration of free Nisin Z and encapsulated with NSs, in particular, Nisin loaded on PMDA-NSs showed better anti-cancer effect against colon (HT-29) cancer that was concordant with other studies outcomes that showed a significant reduction of cell viability in colon [ 56 ], melanoma [ 3 ], and MCF-7 [ 11 ] cancer cells when they were exposed to Nisin-Z, whereas in this study the Nisin Z cytotoxicity effect was remarkably enhanced in complexed with NSs against both cancer cells, which might be related to better entrance of nanosponges into the cells to release Nisin inside the cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…These monomers can be linked to different type of cross-linkers such as carbonyl diimidazole (CDI) and pyromellitic dianhydride (PMDA) to make a highly cross-linked system [ 28 , 29 ]. Previous reports have shown that the main important properties of CDNSs in drug delivery are related to their high capacity to encapsulate different molecules with solubilization improvement power for poorly water-soluble drugs and its properties [ 30 ] protect drugs from physicochemical degradation [ 31 ] and extend the drug release [ 32 ] through intravenous, oral, and pulmonary pathways because they are biodegradable and safe [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Stabilization and Anticancer Enhancing Activity of the Peptide Nisin by Cyclodextrin-Based Nanosponges against Colon and Breast Cancer Cells

et al. 2022

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The great variability of cancer types demands novel drugs with broad spectrum, this is the case of Nisin, a polycyclic antibacterial peptide that recently has been considered for prevention of cancer cells growth. As an accepted food additive, this drug would be very useful for intestinal cancers, but the peptide nature would make easier its degradation by digestion procedures. For that reason, the aim of present study to investigate the protective effect of two different β-cyclodextrin-based nanosponges (carbonyl diimidazole and pyromellitic dianhydride) and their anti-cancer enhancement effect of Nisin-Z encapsulated with against colon cancer cells (HT-29). To extend its possible use, a comparison with breast (MCF-7) cancer cell was carried out. The physicochemical properties, loading efficiency, and release kinetics of Nisin complex with nanosponges were studied. Then, tricin-SDS-PAGE electrophoresis was used to understand the effect of NSs on stability of Nisin-Z in the presence of gastric peptidase pepsin. In addition, the cytotoxicity and cell membrane damage of Nisin Z were evaluated by using the MTT and LDH assay, which was complemented via Annexin-V/ Propidium Iodide (PI) by using flowcytometry. CD-NS are able to complex Nisin-Z with an encapsulation efficiency around 90%. A protective effect of Nisin-Z complexed with CD-NSs was observed in presence of pepsin. An increase in the percentage of apoptotic cells was observed when the cancer cells were exposed to Nisin Z complexed with nanosponges. Interestingly, Nisin Z free and loaded on PMDA/CDI-NSs is more selectively toxic towards HT-29 cells than MCF-7 cancer cells. These results indicated that nanosponges might be good candidates to protect peptides and deliver drugs against intestinal cancers.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stabilization and Anticancer Enhancing Activity of the Peptide Nisin by Cyclodextrin-Based Nanosponges against Colon and Breast Cancer Cells

et al. 2022

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“…The formulations were prepared by polymer condensation, and interfacial phenomenon, and were effective for 6 months at a condition of 40°C and 75% of relative humidity [66]. This type of CD NSs was further used to deliver and increase the activity of several anticancer drugs such as paclitaxel [67], bortezomib [68], flutamide [69], tamoxifen [70], etc. Following their high potential to entrap a variety of molecules, the β-CD/CDI NSs were also used to deliver and improve the solubility and dissolution of paliperidone, an antipsychotic drug for the treatment of schizophrenia [71].…”

Section: Cd-based Polycarbonate Nssmentioning

confidence: 99%

Strategies to Develop Cyclodextrin-Based Nanosponges for Smart Drug Delivery

Hoti¹,

Appleton²,

Pedrazzo³

et al. 2022

Smart Drug Delivery

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In recent years, the development of various cyclodextrin (CD)-based nanosponges (NSs) has gained great importance in the controlled and-or targeted release of drugs due to their versatility and simple preparation. In this chapter, an introduction of different administration routes is explained. Further, different ways to obtain CD-NSs and their classification are shown with a brief explanation of the characterization of the inclusion complexes. Finally, illustrative examples in diverse processes or diseases will be reviewed and explained to demonstrate the potential of CD-NSs. Therefore, this division will serve to compile information on CD-NSs in recent years and to illustrate to readers how to generate and apply different derivatives of interest.

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Nanosponges as an Emerging Platform for Cancer Treatment and Diagnosis

Gowda,

Ahmed,

Almoyad

et al. 2023

Adv Funct Materials

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Nanosponges are unique sponge‐like carrier systems with numerous cavities and a mesh‐like network throughout their surface. They are categorized into three types based on the material of construction, i.e., polymer‐, inorganic‐, and bio‐derived material‐based nanosponges. Unlike conventional nanoparticles, nanosponges bear countless interconnected voids and mesh‐like network that enables maximum drug, imaging probe, or photosensitizer loading via absorption, encapsulation, conjugation, or complexion. The voids can be further sealed using suitable lipids or polymers to avoid leakage of drugs at the non‐target site, protect therapeutics from biological conditions, and control the release of therapeutics for a prolonged period. Recently, nanosponges have grasped the attention of researchers in the area of cancer by overcoming the drawbacks associated with the conventional delivery of immunotherapeutic, targeted therapeutic, and chemotherapeutic agents. Interestingly, several reports have also witnessed their potential in cancer theranostics. Owing to this supremacy, nanosponges could be an apt platform for cancer therapy and diagnosis. The current review discusses the general aspects, types, and preparation methods of nanosponges. In addition, the ambit of nanosponges in treating different types of cancer is meticulously discussed, along with detailed descriptions of their corresponding patents. Furthermore, the role of nanosponges in phototherapy and cancer theranostics are selectively canvassed.

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In-vitro characterization and cytotoxicity study of flutamide loaded cyclodextrin nanosponges

Cited by 24 publications

References 21 publications

Stabilization and Anticancer Enhancing Activity of the Peptide Nisin by Cyclodextrin-Based Nanosponges against Colon and Breast Cancer Cells

Stabilization and Anticancer Enhancing Activity of the Peptide Nisin by Cyclodextrin-Based Nanosponges against Colon and Breast Cancer Cells

Strategies to Develop Cyclodextrin-Based Nanosponges for Smart Drug Delivery

Nanosponges as an Emerging Platform for Cancer Treatment and Diagnosis

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