In vitro blood brain barrier exposure to mycotoxins and carotenoids pumpkin extract alters mitochondrial gene expression and oxidative stress

Alonso-Garrido, Manuel; Frangiamone, Massimo; Font, Guillermina; Cimbalo, Alessandra; Manyes, Lara

doi:10.1016/j.fct.2021.112261

Cited by 18 publications

(7 citation statements)

References 43 publications

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“…Pumpkin, which belongs to the Cucurbitaceae family, is rich in carotenoids (i.e., lycopene, αand β-carotene, lutein, and zeaxanthin) that play an important role in protecting cells from oxidation and cellular damage, preventing the incidence of human diseases such as mutagenic processes, cardiovascular diseases, osteoporosis, and diabetes [24]. Recent studies have shown that carotenoids-rich food such as pumpkin could counteract the toxic effects produced by mycotoxins [25,26]. Moreover, carotenoids revealed the ability of reducing mycotoxins such as AFB1 in rat tissues [27].…”

Section: Introductionmentioning

confidence: 99%

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Escrivá

Agahi

Vila‐Donat

et al. 2021

Toxins

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The presence of mycotoxins in cereals and cereal products remains a significant issue. The use of natural ingredients such as pumpkin and whey, which contain bioactive compounds, could be a strategy to reduce the use of conventional chemical preservatives. The aim of the present work was to study the bioaccessibility of aflatoxin B1 (AFB1) and ochratoxin (OTA) in bread, as well as to evaluate the effect of milk whey (with and without lactic acid bacteria fermentation) and pumpkin on reducing mycotoxins bioaccessibility. Different bread typologies were prepared and subjected to an in vitro digestion model. Gastric and intestinal extracts were analyzed by HPLC–MS/qTOF and mycotoxins bioaccessibility was calculated. All the tested ingredients but one significantly reduced mycotoxin intestinal bioaccessibility. Pumpkin powder demonstrated to be the most effective ingredient showing significant reductions of AFB1 and OTA bioaccessibility up to 74% and 34%, respectively. Whey, fermented whey, and the combination of pumpkin-fermented whey showed intestinal bioaccessibility reductions between 57–68% for AFB1, and between 11–20% for OTA. These results pointed to pumpkin and milk whey as potential bioactive ingredients that may have promising applications in the bakery industry.

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Section: Introductionmentioning

confidence: 99%

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Escrivá

Agahi

Vila‐Donat

et al. 2021

Toxins

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“…Most intracellular ROS production under normal conditions is related to mitochondria [ 34 ]. Previous studies have shown that ZEA affects the activity, number, structure, and function of mitochondria and also affects the electron transport chain [ 35 , 36 , 37 , 38 ]. ZEA-induced mitochondrial damage may be an important pathway for promoting ROS production [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Heme Oxygenase-1 Regulates Zearalenone-Induced Oxidative Stress and Apoptosis in Sheep Follicular Granulosa Cells

Li,

Gao,

Yao

et al. 2024

IJMS

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Zearalenone (ZEA) is a common non-steroidal estrogenic mycotoxin found in a range of animal feeds and poses a serious threat to the reproductive health of farm animals and humans. However, the mechanism underlying ZEA-induced reproductive toxicity in sheep remains unknown. Granulosa cells are crucial for egg maturation and the fertility of female sheep. In this study, we aimed to examine the impact of different ZEA concentrations on sheep follicular granulosa cells and to elucidate the potential molecular mechanism underlying ZEA-induced toxicity using transcriptome sequencing and molecular biological approaches. Treating primary sheep follicular granulosa cells with different concentrations of ZEA promoted the overproduction of reactive oxygen species (ROS), increased lipid peroxidation products, led to cellular oxidative stress, decreased antioxidant enzyme activities, and induced cell apoptosis. Using transcriptome approaches, 1395 differentially expressed genes were obtained from sheep follicular granulosa cells cultured in vitro after ZEA treatment. Among them, heme oxygenase-1 (HMOX1) was involved in 11 biological processes. The protein interaction network indicated interactions between HMOX1 and oxidative and apoptotic proteins. In addition, N-acetylcysteine pretreatment effectively reduced the ZEA-induced increase in the expression of HMOX1 and Caspase3 by eliminating ROS. Hence, we suggest that HMOX1 is a key differential gene involved in the regulation of ZEA-induced oxidative stress and apoptosis in follicular granulosa cells. These findings provide novel insights into the prevention and control of mycotoxins in livestock.

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“…However, the favourable effect of AE supplementation might also result from the presence of other ingredients, including pectins, triterpenes, carotenoids, vitamin C, and vitamin E, as well as minerals [ 47 , 48 ]. Because chokeberries and their products contain other ingredients known for antioxidative action in the brain, such as vitamin E, zinc, carotenoids, and selenium [ 33 , 62 , 63 , 64 ], the protective impact of the extract was probably not only the effect of polyphenols but also of the other ingredients characterized with reductive capacity. Moreover, it seems possible that because of their same mode of action, some components of the extract might act synergistically.…”

Section: Discussionmentioning

confidence: 99%

“…It seems that the role of quercetin in this protection may be important because cadmium damages the blood–brain barrier [ 5 , 27 ]. Moreover, vitamin E, as a lipid-soluble compound, can cross the blood–brain barrier [ 70 ] and thus it acts directly in the brain, whereas β-carotene not only can cross this barrier, but also protect against its damage [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

The Protective Impact of Aronia melanocarpa L. Berries Extract against Prooxidative Cadmium Action in the Brain—A Study in an In Vivo Model of Current Environmental Human Exposure to This Harmful Element

Ruczaj,

Brzóska,

Rogalska

2024

Nutrients

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Cadmium (Cd) is a prooxidant that adversely affects human health, including the nervous system. As exposure of the general population to this heavy metal is inevitable, it is crucial to look for agents that can prevent the effects of its toxic action. An experimental model on female rats of current lifetime human exposure to cadmium (3–24-months’ treatment with 1 or 5 mg Cd/kg diet) was used to test whether low-level and moderate intoxication can exert a prooxidative impact in the brain and whether supplementation with a 0.1% extract from the berries of Aronia melanocarpa L. (Michx.) Elliott (AE; chokeberry extract) can protect against this action. Numerous parameters of the non-enzymatic and enzymatic antioxidative barrier, as well as total antioxidative and oxidative status (TAS and TOS, respectively), were determined and the index of oxidative stress (OSI) was calculated. Moreover, chosen prooxidants (myeloperoxidase, xanthine oxidase, and hydrogen peroxide) and biomarkers of oxidative modifications of lipids, proteins, and deoxyribonucleic acid were assayed. Cadmium dysregulated the balance between oxidants and antioxidants in the brain and led to oxidative stress and oxidative injury of the cellular macromolecules, whereas the co-administration of AE alleviated these effects. To summarize, long-term, even low-level, cadmium exposure can pose a risk of failure of the nervous system by the induction of oxidative stress in the brain, whereas supplementation with products based on aronia berries seems to be an effective protective strategy.

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In vitro blood brain barrier exposure to mycotoxins and carotenoids pumpkin extract alters mitochondrial gene expression and oxidative stress

Cited by 18 publications

References 43 publications

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Heme Oxygenase-1 Regulates Zearalenone-Induced Oxidative Stress and Apoptosis in Sheep Follicular Granulosa Cells

The Protective Impact of Aronia melanocarpa L. Berries Extract against Prooxidative Cadmium Action in the Brain—A Study in an In Vivo Model of Current Environmental Human Exposure to This Harmful Element

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