In vitro biocompatibility of electrospun poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fiber mats

Suwantong, Orawan; Waleetorncheepsawat, Suchada; Sanchavanakit, Neeracha; Pavasant, Prasit; Cheepsunthorn, Poonlarp; Bunaprasert, Tanom; Supaphol, Pitt

doi:10.1016/j.ijbiomac.2006.07.006

Cited by 127 publications

(91 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Further, when CHX is added to the PHB/PEO polymer blend, the deformation at break is reduced but the elastic modulus increases (table I). PHB films are known to have fragile behavior with a deformation at break below 5 % and an elastic modulus around 1.5 GPa [29,30]. The brittleness of PHB was attributed to polymer glass transition that occurs ~ 0 ºC, leading to cold recrystallization at room temperature, which restricts the mobility of the amorphous phase in interfibrillar and/or interlamellar regions, and results in a failure at relatively low strains [31].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications

et al. 2014

View full text Add to dashboard Cite

Fiber meshes of poly(hydroxybutyrate) (PHB) and poly(hydroxybutyrate)/ poly(ethylene oxide) (PHB/PEO) with different concentrations of chlorhexidine (CHX) were prepared by electrospinning, for assessment as a polymer based drug delivery system. The electrospun fibers were characterized at morphological, molecular and mechanical levels. The bactericidal potential of PHB and PHB/PEO electrospun fibers with and without CHX was investigated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by disk diffusion susceptibility tests. Electrospun fibers containing CHX exhibited bactericidal activity. PHB/PEO-1%CHX displayed higher CHX release levels and equivalent antibacterial activity when compared to PHB/PEO with 5 and 10 wt% CHX. Bactericidal performance of samples with 1 wt% http://www.sciencedirect.com/science/article/pii/S0142941813002493 2 CHX was assessed by Colony Forming Units (CFU), where a reduction of 100 % and 99.69 % against E. coli and S. aureus were achieved, respectively.

show abstract

Section: Mechanical Propertiesmentioning

confidence: 99%

PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications

et al. 2014

View full text Add to dashboard Cite

show abstract

“…PHB has attracted the attention of tissue engineers and has become the most widely studied member of the PHA family. Moreover, PHB is known to be biocompatible to various cell lines and no report exists of its toxicity in medical applications (Suwantong et al, 2007;da Silva et al, 2016). These properties make it suitable for a variety of applications in the health industry, such as controlled release systems (Daranarong et al, 2014), surgical sutures (Fedorov et al, 2007), wound dressings (Rennukka et al, 2014), and orthopedic uses (Alves et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cell-compatible PHB/silk fibroin composite nanofibermat for tissue engineering applications

Karahaliloğlu¹

2017

Turk J Biol

View full text Add to dashboard Cite

Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin (SF) is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[(R)-3-hydroxybutyrate] (PHB) has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell-biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were prepared through the use of the electrospinning technique, which is a unique method for the production of biomaterials on the nanoscale intended for tissue engineering. PHB/SF scaffolds were prepared with different SF contents and a suitable electrospinning condition was chosen in terms of structure and fiber diameter. The average fiber diameter was 92 ± 2.6 nm at a flow rate of 0.4 mL/h, distance of 20 cm, polymer concentration of PHB/SF-0 of 7%:18% (w/w) or 1:1 (v/v), and voltage of 20 kV. Mechanical and crystalline properties of the PHB/SF scaffold were investigated. Adhesion and proliferation of L929 and HaCaT (mouse fibroblast and immortalized human keratinocyte cell lines) on PHB/SF-0 were examined.

show abstract

“…It is well known that biocompatibility of bio materials depends not only on the chemical structure but, to a large extent, on the size. Suwantong and coworker could show using indirect cytotoxicity assessment that PHB fibre mats were acceptable to both mouse L929 fibroblast cells and mouse Schwann cells (RT4-D6P2T) (Suwantong et al 2007). Mouse fibroblast cells cultured with polymeric PHB microspheres revealed no cytotoxic effects which were indicated by the absence of changes in the cell morphology, cell viability and proliferative activity .…”

Section: Poly-3-hydroxybutyrate (Phb)mentioning

confidence: 99%

“…sugar and molasses, as intracellular storage materials. PHB is an ideal biomaterial, because it is a biodegradable polyester and completely degrades in D,L-b-hydroxybutyrate (HB), a normal component of blood and tissue (Miller and Williams 1987;Suwantong et al 2007). PHB is perfectly isotactic viz.…”

Section: Poly-3-hydroxybutyrate (Phb)mentioning

confidence: 99%

Biomaterials Applicable for Alveolar Sockets Preservation: In Vivo and In Vitro Studies

Kunert‐Keil¹,

Gredes²,

Gedrange³

2011

Implant Dentistry - The Most Promising Discipline of Dentistry

View full text Add to dashboard Cite

In vitro biocompatibility of electrospun poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fiber mats

Cited by 127 publications

References 25 publications

PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications

PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications

Cell-compatible PHB/silk fibroin composite nanofibermat for tissue engineering applications

Biomaterials Applicable for Alveolar Sockets Preservation: In Vivo and In Vitro Studies

Contact Info

Product

Resources

About