In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island

Abstract

Sorbus commixta, a flowering plant in the Rosaceae family, is native to Japan and Ulleung Island, Korea. This plant is also called maga-mok or mai-mok in Korea because the bud of the stem has a similar shape to the teeth of a horse. In this study, hot water extracts from different parts of S. commixta, such as leaf, stem, and immature and mature fruits, were prepared, and their antithrombosis and antioxidant activities were evaluated. The extraction yield and pH of stem extracts were 3.99% and 5.5, respectively. The stem extracts contained 89.2 mg/g of total polyphenols and 28.3 mg/g of total flavonoids. The hot water extracts prepared from the leaf, stem, immature, and mature fruit of S. commixta exhibited no hemolytic activity against human red blood cells, up to a concentration of 0.5 mg/ml. In an anticoagulation assay, the stem extracts showed strong extension in thrombin, prothrombin, and activated partial thromboplastin times, whereas the other extracts had no anticoagulation activity. In a platelet aggregation inhibitory activity assay, all the extracts tested had no inhibitory activity against human platelets. With regard to antioxidation activity, the stem extracts showed stronger radical scavenging activity and reducing power activity than the other extracts. The calculated RC50s, the concentration required for 50% radical scavenging activity, for DPPH anions, ABTS cations, and nitrite of the crude stem extracts were 119.7, 53.3, and 117.5 μg/ml, respectively, whereas they were 13.7, 5.2, and 14.9 μg/ml for DPPH anions, ABTS cations, and nitrite, respectively, for vitamin C. The results suggest that the stem extracts of S. commixta have strong potential for use as a novel resource for antithrombosis agents.Key words : Anticoagulation, anti-platelet aggregation, Sorbus commixta, stem extracts, Ulleungdo *Corresponding author *Tel : +82-54-820-5491, Fax : +82-54-820-7804 *E-mail : hysohn@anu.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

A New Dibenzofuran from the Barks of Sorbus commixta

“…The anticancer activity of the bars of S. commixta has been previously reported, [7] but whether single compounds have the anti-proliferation activity against cancer cells have not reported yet. Previous research on the plant had reported the isolation of sorbic acid, chalcone glucoside, prunasin, amygdalin and triterpenoids such as lupenone and lupeol [4,[8][9]. Recently, the methanol extract from the barks of S. commixta was shown to have antioxidant, anti-inflammatory and the potent radical scavenging activity [4,5].…”

“…It has used for the treatment of neuralgia, cough and expectorant in folk remedies [1][2][3]. Until now, the study on the S. commixta has been mainly focused on the berries of S. commixta, and their biological activities, antiinflammatory and antioxidant effect, have been studied [4][5][6]. The anticancer activity of the bars of S. commixta has been previously reported, [7] but whether single compounds have the anti-proliferation activity against cancer cells have not reported yet.…”