In vitro antioxidant potential and phytochemical profiling of Melastoma malabathricum leaf water extract

Abstract

Melastoma malabathricum is one of the medicinal plants plated in West Kalimantan, Indonesia. Its leaves extraction using organic solvents revealed bioactive compounds and antioxidant activity. However, the antioxidant activity, toxicity, and chemical profile of water extract of M. malabathricum leaves are still unknown. This study aimed to determine extraction conditions that provide water extract of M. malabathricum with the highest antioxidant activity and the lowest toxicity. The extraction conditions were conducted at different water temperatures (25 ± 2 ºC and 90 ± 2 ºC), concentrations (10 and 5 g leaves/100 mL water), and extraction time (15, 30, and 45 minutes). Data analysis was performed by one-way ANOVA followed by Duncan's test (p < 0.05). The result showed that M. malabathricum leaves of 5g/100 mL extracted with hot water (90 ± 2 ºC) for 15 minutes showed the highest antioxidant activity (IC50 2.13 ± 19.20 ppm), which was higher than vitamin C (IC50 4.32 ± 0.16 ppm), and low toxicity (LC50 333.06 ± 99.45 ppm). Six compounds namely, 4-O-caffeoylquinic acid, quercimeritin, digiprolactone, 3-O-trans-coumaroylquinic acid, norbergenin, and arteamisinin were identified for the first time in the water extract of M. malabathricum leaves. These compounds, individually or extracted from other ingredients, have previously been reported to have antioxidant activity.

Phytochemical Profile and Biological Activities of Ethylacetate Extract of Peanut (Arachis hypogaea L.) Stems: In-Vitro and In-Silico Studies with Bibliometric Analysis

“…Interpreting all data and comparing them with references resulted in compound structures like the names obtained from LC-MS/MS (Table 3) and the fragmentation patterns in Figures 10-19. Compound 1 is cis-Caffeic acid (Santos et al, 2013), 2 (Baptifoline) (Chang et al, 2016), 3 (Arteamisinine I) (Oke et al, 2022), 4 (Harmol) (Fahmy et al, 2021), 5 (1,4-Dihydroxy-2-methyl-anthraquinone) (Saha et al, 2015), 6 (3R)-5′-Methoxy-vestitol (Bankova et al, 2016), 7 (5,7-Dihydroxy-3-(4'hydroxybenzyl) chromone (Robbani et al, 2022), 8 (Aschantin) (Patyra et al, 2022), and 9…”

PROFIL SENSORI TEH DAUN KARAMUNTING (Melastoma malabathricum L.) DENGAN METODE SENSOMETRIK

“…Studi pada teh hijau yang menunjukkan bahwa selain epicatechin, flavonol glycosides (myricetin-3-O-galactoside dan quercetin-3-O-rutinoside) juga memiliki korelasi positif (r=0,7878, p<0,01) dengan astringency (Xu et al, 2018). Ekstrak air dari daun karamunting mengandung quercimeritrin (Lestari et al, 2022). Senyawa tersebut termasuk dalam flavonol glycosides dengan nama lain quercetin 7-O-glucoside yang ditandai dengan gugus glucoside pada cincin A di C7.…”

“…Metode tersebut dapat menurunkan atribut sensori yang tidak disukai, seperti astringency dan bitter (Lin et al, 2014). Potensi fungsional pada daun karamunting yang diaplikasikan sebagai minuman telah diteliti diantaranya sebagai antihiperglikemik (Truong, 2020) dan antioksidan (Lestari et al, 2022), namun belum diketahui atribut sensori dari aplikasi minuman fungsional tersebut. Konsumen menyukai teh dengan rasa yang tidak terlalu bitter dan kurang astringency (Yang dan Lee, 2020).…”

The Effect of Character and Local Wisdom-Integrated Causalitic Model Learning on Problem-Solving Skills and the Development of Environmental Conservation Literacy

“…Hunaepi et al (2018) state that integrating local wisdom into education makes students appreciate local culture, builds their character, stimulates curiosity, and fosters a desire to solve problems through critical thinking. Additionally, according to Lestari & Yuliana (2014), character education, especially environmental care, can make students more environmentally conscious and capable of preserving the environment. One of the functions of local wisdom is conservation and preservation of natural resources (Maridi, 2015).…”