In vitro anticancer activity of natural β-carotene from Dunaliella salina EU5891199 in PC-3 cells

Apoptosis Comparison Effects Between Synthetic and Natural Β-Carotene from Dunaliella salina on MDA-MB-231Brest Cancer Cells

“…Obtained results in MDA-MB-231 Brest cancer cells shown that β-carotene from Dunaliella induced 70% cells death with respect to synthetic molecule that only presented 30% in MTT assays (Figure 7a). These results are in agreement with cell and animal model trials published, where induction of apoptosis at different levels was carried out by D. salina extracts [3][4][5]18]. Nevertheless, no one of the mentioned authors had worked with MDA-MB-231 Brest cancer cells neither HaCat, only Prakash and coworkers made some β-carotene inhibition test in MDA-MB-231 cells, but non-results were reported [42].…”

“…Additionally, it is important to point out that failed human clinical trials in Finland and USA (ATβC and CARET), were developed using high levels of synthetic β-carotene and without inclusion of 9CβC [6]. In this sense, clinical trials development using natural β-carotene with high levels of 9-cis isomer are recommended, due results obtained in this work and other reports published [3][4][5]18]. For this reason, a culture medium specifically formulated to overproduce 9CβC in D. salina, as the one developed in this work is required.…”

“…Breast cancer is a complex disorder that can occur because of vitamin deficiencies and due process including oxidative stress and lipid peroxidation. Biological mechanisms exist to support anticancer properties of natural antioxidant pro-vitamin A molecule (β-carotene) in animals and overall impression is that intake of it, play a substantial role in prevention of cancer [3][4][5]. However, anticancer effects are not so positive in humans using synthetic β-carotene molecule [6][7][8].…”

“…Red flagellated microalgae strains contained in samples were morphologically and molecularly identified as Dunaliella, before purification and growth. Samples containing D. salina were serially diluted up to 10 −4 and 0.1 ml spread on 2% De Walne's agar medium [3,28]. Distinct colonies developed on plates were transferred to β-carotene inducing medium supplemented with 1.0, 1.5 and 2 M of NaCl and kept at 24 ± 1 o C in thermostatically controlled chamber, illuminated with four cool fluorescent lamps to generate an irradiance of 200 µE m-2 s-1, under 12 h/12 h light/dark photo period.…”

Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in <i>Dunaliella salina </i>A9

“…However carotenoid and lipid accumulation of Dunaliella salina A9 were the highest with the hypo-osmotic stress (0.5M) and nutrient starvation. Previous studies demonstrated the antioxidant [27,35,37,40], antimicrobial [12,23] and anticancer [18] properties of the extract from Dunaliella salina under the stress conditions. Dunaliella salina A9 can apply to functional food and biofuel in Viet Nam, because of capacity of higher carotenoid and lipid accumulation under stress conditions.…”