In vitro anti-HIV-1 activity of fucoidan from Sargassum swartzii

Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification

“…The extraction of macroalgal polysaccharides is mainly carried out by solvent extraction, with a wide variation in the conditions and methods used in the literature to date as seen in (Cong et al, 2016;Dinesh et al, 2016;Yuan & Macquarrie, 2015). Other authors described the use of CaCl 2 solutions at different concentrations and temperatures as an alternative to the initial water or slow acidic conditions to obtain alginates, laminarin and fucoidan in a single step and separate them further by applying several consecutive purification steps (Lim et al, 2014).…”

“…The elution of the adsorbed fucoidan from different resins was performed by stepwise (Anastyuk, Imbs, Shevchenko, Dmitrenok, & Zvyagintseva, 2012;Cong et al, 2016) or linear (Dinesh et al, 2016;Imbs et al, 2016;Menshova et al, 2015; NaCl salt gradient. Different fucoidan fractions could be further separated by AEC based on the degree of sulphation of the molecules.…”

“…Supernatants mixed with CaCl 2 (4%, 4˚C, overnight) followed by centrifugation and ethanol precipitation of supernatant and dialysis of the pellet. Dinesh et al (2016)…”

Binding property of HIV p24 and Reverse transcriptase by chalcones from Pongamia pinnata seeds

“…Briefly, 50 μg to 1000μg of different concentrations (50, 100, 200, 400, 800, 1000 μg) of aqueous extracts were used for screen anti HIV-1 inhibition assays. For the anti-HIV testing HIV-1 (Clade C), strain was infected with human PBMCs and TCID 50 was calculated as described in our earlier studies [ 4 ] and stored at - 196°C for further use.…”

The development of seaweed-derived bioactive compounds for use as prebiotics and nutraceuticals using enzyme technologies

“…However, some problems of the ultrafiltration method were reported in term of low yield, long time-consuming process, and inaccuracy due to non-spherical shape of polysaccharides (Shi, 2016). Anion-exchange chromatography, particularly DEAE-cellulose, Q-Sepharose Fast Flow, and Macro-Prep DEAE column is also one of the most common purification methods used for fucoidan separation based on its high anionic charges and degree of sulphation (Anastyuk et al, 2017;Wang et al, 2012a;Menshova et al, 2015;Cong et al, 2016;Dinesh et al, 2016;Imbs et al, 2016). In addition, the application of SEC such as Sephacryl S-300 and TSK-GEL column allows fractionation of the fucoidan regarding to their MWs (Cong et al, 2016;Anastyuk et al, 2017).…”