In vitro anther culture of sweet orange (Citrus sinensis L. Osbeck) genotypes and of a C. clementina × C. sinensis ‘Hamlin’ hybrid

Cardoso, Jean Carlos; Martinelli, Adriana Pinheiro; Germanà, María Antonietta; Latado, Rodrigo Rocha

doi:10.1007/s11240-014-0456-x

Cited by 19 publications

(16 citation statements)

References 48 publications

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“…There were two types of anther-derived calli: a hard white-greenish nonmorphogenic callus, similar to ones obtained by Cardoso et al (2014), or highly embryogenic type (Figure 1(E),(F)) with a soft and friable aspect, and with small immature embryos. Rarely, the route of regeneration took place through direct embryogenesis (Figure 1(G)), organogenesis (Figure 1(H)), or through secondary embryogenesis starting at the base of an embryo (Figure 1(I)).…”

Section: Resultssupporting

confidence: 53%

“…In spite of the small number of SSRs analyzed, which does not allow for definitive conclusions, the Gametic and somatic embryogenesis of Citrus 7 identical profile at four loci could indicate a common somatic origin for these cultivars and clones, a common occurrence in many Citrus species as observed by several authors (Hodgson 1967;Cardoso et al 2014). Microsatellites have been previously used to characterize the regenerants obtained from Citrus anther culture (Germanà et al , 2013.…”

Section: Characterization Of Regenerants: Ploidy Analysis and Analysimentioning

confidence: 99%

“…Anthers were squashed in a few drops of DAPI solution (1 mg mL 21 ) and observed under a fluorescent microscope Zeiss Axiophot (Zeiss, Oberrkochen, Germany) to identify the flower bud size containing the highest percentage of vacuolated microspores (5 -7 mm in length for oranges, 3 -6 mm for clementines: Figure 1(B),(C)). This stage (Figure 1(A)) was previously identified as the most responsive for gametic embryogenesis in Citrus (Germanà 2007;Cardoso et al 2014) and were selected for culture. DAPI staining and observation by light and fluorescence microscopy were also performed at different times during the culture to observe changes in microspore development inside anthers.…”

Section: Plant Materialsmentioning

confidence: 99%

“…f. plants (Germanà et al 1991), and haploid Clausena excavata embryos (Froelicher & Ollitrault 2000) have also been obtained via anther culture. Recently, short-lived homozygous plantlets have been recovered from the anthers of Rhode Red Valencia sweet orange (Cao et al 2011), andCardoso et al (2014) obtained homozygous callus from a hybrid between C. clementina £ C. sinensis "Hamlin". However, C. sinensis can still be considered a very recalcitrant species to gametic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos).

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Section: Resultssupporting

confidence: 53%

Section: Characterization Of Regenerants: Ploidy Analysis and Analysimentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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“…More recently, homozygous plantlets have been recovered from the anthers of 'Rhode Red Valencia' sweet orange (Cao et al 2011), such as homozygous callus from cv. 'Hamlin' (C. clementina × C. sinensis) (Cardoso et al 2014), homozygous microspore-derived embryos from cvs. 'Monreal Rosso' and 'Nules' (Chiancone et al 2015) and homozygous and triploid regenerants of 'Hernandina' and 'Corsica' clementines (Cardoso et al 2016), were also obtained.…”

Section: Mandarin Isolated Microspore Culturementioning

confidence: 99%

Ploidy manipulation for genetic improvement in some Mediterranean fruit crops

Cimò¹

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I ACKNOWLEDGMENTS I am extremely grateful to my supervisor, Prof. Maria Antonietta Germanà, for giving me her trust, for her patience, understanding and affection, for her expert guidance, time and dedication in my training and planning, development and completion of this thesis.I would like to thank all the staff and colleagues of the 'Università degli Studi di Palermo' for their technical assistance, help and cooperation during my research work.I would like to express my sincere gratitude to my co-supervisor, Dra Marisa Badenes, for making me feel part of her team, for all her efforts, support, constant encouragement and useful suggestions.I am thankful to Mar Naval for her guidance, patience and persistence during my invaluable experience at the 'Instituto Valenciano de Investigaciones Agrarias' (IVIA).I would like to thank all the working group of the 'Fruit Tree Breeding Department' of the IVIA for their cooperation, hospitality and friendship, and to the 'Tissue Culture' group, especially to Pablo Aleza, for his help and disinterested advice.I am grateful to Manuel Agustí, Carmina Reig, Carlos Mesejo and all the working group of the 'Instituto Agroforestal Mediterráneo' (IAM) for kindly welcoming and supporting me during my stay at the 'Universitat Politècnica de València' (UPV).I am thankful to Jose M. Seguí-Simarro and all the group of the 'Instituto para la Conservación y la Mejora de la Agrodiversidad Valenciana' (COMAV) for their dedication in my training and improvement on the isolated microspores in vitro culture techniques.Special thanks to all my friends and to all the amazing people I have met during these years, who have supported me and led me thus far.Many thanks to my family for having always encouraged and supported me in all my choices and for their great endurance during my long stay away from them.To Ana Luisa, for all the love you gave me, for being always remained at my side, for your endless and invaluable presence. All this would not be possible without you... Todo llega para el que sabe esperar.( Pherograms of the microsatellite markers UDAP-468 profiles of the mother plant (top) and two 'Filippo Ceo' regenerants. The mother plant is heterozygous and carries two alleles, the regenerants show only one of the mother plant alleles. The presence of alternative alleles from the mother plant has been considered as support for the gametic origin of regenerants 51 Table 4.2 Anthers culture responses to the seven genotypes tested 81 Table 4.3 Anthers culture responses to the four culture media tested three months after culture 83 Regarding the almond anther culture, formation of calli and production of embryos was achieved through the direct embryogenesis route. On then other hand, early embryo regeneration is reported, for the first time, from isolated microspore culture of mandarin, 'Mandarino Tardivo di Ciaculli'.Our results report the evidence of gametic embryogenesis and the production of homozygous regenerants in almond and mandarin, two species extremely recalcitrant to micr...

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Microspore Embryogenesis in Citrus

Yahyaoui

Germana

2021

Methods in Molecular Biology

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In vitro anther culture of sweet orange (Citrus sinensis L. Osbeck) genotypes and of a C. clementina × C. sinensis ‘Hamlin’ hybrid

Cited by 19 publications

References 48 publications

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Ploidy manipulation for genetic improvement in some Mediterranean fruit crops

Microspore Embryogenesis in Citrus

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