In vitro and in vivo study of carboxymethyl chitosan/polyvinyl alcohol for wound dressing application

Abstract

Antibacterial, good biosafety, and wound healing enhancers, are the most favorable factors for wound dressing. Designing nanomaterial of the scaffold for supporting skin regeneration is a complicated process. Carboxymethyl chitosan (CMC) structure similar to the glycosaminoglycan and antibacterial activity combined with polyvinyl alcohol (PVA) (PVA/CMC) can lead to satisfactory properties for wound management. In this study, a collection of electrospun nanofibrous scaffolds are fabricated by blending the constant PVA concentration (9%) with a limited area of CMC concentration (2%, 3%, 4%, and 5%). PVA/CMC scaffolds characteristic was investigated through a scanning electron microscope, Fourier‐transform infrared spectroscopy, and contact angle tests. The fiber diameter values were measured for PVA (144.60 nm), chitosan (131.57 nm), and average different concentration of CMC in PVA/CMC (104.7 ± 2.45 nm). In vitro potential of the scaffolds were evaluated by using human dermal fibroblast cells and human placental‐derived mesenchymal stem cells. According to in vitro results, PVA/CMC4% scaffold was selected as the optimized wound dressing for skin tissue engineering. The in vivo wound healing data in rat models illustrated more healing capacity of PVA/CMC4% dressing in which accelerating wound closure and freshly healed tissue with high similarity to the normal skin was reorganized.

Therapeutic effects of electrospun chitosan nanofibers on animal skin wounds: A systematic review and meta-analysis

“…Most of the identified articles are from research cores from different countries, mainly Iran and China (Fig. S3) ( Akbari et al, 2022 ; Mirmajidi et al, 2021 ; Zhang et al, 2022 ). The number of articles published in the last 10 years demonstrates the existence of evidence-based knowledge about the application of QSNFs and their therapeutic effects in veterinary medicine, enough to perform a meta-analysis of the use of this novel technique and its involved biopolymers (Fig.…”

Hyaluronic acid/lactose-modified chitosan electrospun wound dressings – Crosslinking and stability criticalities

“…ATR-FTIR spectra of CDI-crosslinked and un-crosslinked HA/CTL/PEO mats, compared with the spectra of the single membrane constituents (that are hyaluronic acid, CTL, PEO) and of CDI (in the case of crosslinked mats). literature following the most varied timepoints, from 4 h to 12-16 h to 3 days (Akbari et al, 2022;Ali et al, 2022;Qian et al, 2011). Two different strategies were here attempted by using glutaraldehyde vapor at different times (namely, 4 h and 2 h), in combination with heat treatment in the case of 2 h crosslinking for further stabilization.…”

Integration of Electrospun Scaffolds and Biological Polymers for Enhancing the Delivery and Efficacy of Mesenchymal Stem/Stromal Cell Therapies

“…Examples of GAGs that have been added to electrospun scaffolds for MSC delivery include chondroitin sulfate (CS) [49] and hyaluronic acid (HA) [50]. Apart from animal-derived polysaccharides, recent studies have also explored the use of other nature-derived polymers, such as alginate [51,52], cellulose [53], and chitosan [54].…”