In vitro and in vivo antioxidant properties and DNA damage protective activity of green fruit of Ficus glomerata

Verma, Arti R.; Vijayakumar, M.; Rao, Chandana Venkateswara; Mathela, C. S.

doi:10.1016/j.fct.2009.11.052

Cited by 57 publications

(39 citation statements)

References 26 publications

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“…Many antioxidant compounds, naturally occurring in plant sources have been identified as free-radical scavengers (Verma et al, 2010). A number of synthetic antioxidants, such as 2-and 3-tert-butyl-4-methoxyphenol (butylated hydroxyanisole; BHA) and tert-butylhydroquinone have been added to foodstuffs, but because of…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antioxidant and free-radical scavenging potential ofArtemisia absinthium

Bora¹,

Sharma²

2011

Pharmaceutical Biology

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Section: Introductionmentioning

confidence: 99%

Evaluation of antioxidant and free-radical scavenging potential ofArtemisia absinthium

Bora¹,

Sharma²

2011

Pharmaceutical Biology

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“…[32,33] In case of inhibition of hydroxyl radicals, fractions containing diethyl ether and ethyl acetate showed maximum activity, may be because of their higher ability to bind iron ion and potential for direct scavenging activity on OH -, which revealed the same results as Verma et al, 2010. [34] was recorded in Fr-F (67.89%) and lowest in Fr-A (8.27%).…”

Section: Wwwantioxorgmentioning

confidence: 99%

Phytochemical Screening and ln-vitro Antioxidant Profiling of Solvent Fractions of Canna edulis Ker Gawler

Mishra

Sen

2012

Free Radicals and Antioxidants

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13Fr e e R a d . A n t i ox .Re s e a r c h A r t i c l e West Bengal, apdas.nbu@gmail.com; senarnab_nbu@hotmail.com INTRODUCTIONOxygen plays a vital role in the survival of life on earth. However, during it's utilization in various life processes, a tiny amount of oxygen gets reduced to different free radicals like hydrogen peroxide, superoxide, nitric oxide, hydroxyl radicals etc. [1,2] All these reactive oxygen radicals are accountable for various health hazards to human life causing the development of degenerative diseases.[3] Antioxidant molecules, on the other hand, can slow down or cease the activity of these harmful oxygen species by being oxidizing themselves. Plants often contain good amount of natural antioxidants. [4,5] Different phytochemicals like phenols, flavonols, carotenoids, proanthocyanidins, vitamin C and E can be utilized to scavenge the excess free radicals from human body. [6] Canna edulis Ker Gawler, is a medium sized perennial flowering plant found in the north eastern Himalayan regions of India.[7] The plants bear broad, flat, blackish green leaves with spectacular red flowers. Besides, it also tickles our test buds as it is known to be edible and mainly consumed by the Indian tribes like Lepchas, Bhutias and Nepalis. [8] Different parts of the plants like rhizomes, leaves and seeds are used as medicines by the tribal communities of North eastern India.[9] Amongst various plant parts the extracts of rhizomes are rich source of polyphenolic compounds like phenols and flavonoids, [10] which in turn provide the evidences of antioxidative properties of the plant. ABSTRACT Introduction:Canna edulis Ker Gawler, grown in the sub Himalayan region containing starchy rhizome are used by the tribal people as food and herbal medicine. Methods: Investigations were done to find out 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity, total flavonols and total proanthocyanidin contents, nitric oxide (NO) scavenging activity, hydroxyl radical scavenging activity of different solvent fractions of aquous extract of C. edulis rhizome. Among the cellular molecules, lipids containing unsaturated fatty acids with more than one double bond are particularly susceptible to action of free radicals. The resulting reaction, known as lipid peroxidation and anti lipid peroxidation activity has been performed using goat liver homogenate. Results: Six out of 29 fractions showed DPPH free radical inhibition above 75% and were used for further phytochemical screening. Diethyl ether : ethyl acetate (1:3) fraction showed the maximum inhibition percent. Highest amount of total flavonol and total proanthocyanidins were recorded in diethyl ether : ethyl acetate (1:3) fraction. Maximum NO scavenging activity and hydroxyl radical inhibition activity were observed in bioactive diethyl ether : ethyl acetate (1:1) fraction. Inhibition of lipid peroxides was maximum in ethyl acetate fraction. Data from thin layer chromatography (TLC) revealed the presence of phenolic compounds in all fractions, whereas flavono...

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“…Natural antioxidants are in high demand for application as nutraceuticals, bio-pharmaceuticals, as well as food additive because of consumer preference. 1,2 Among dietary antioxidants, phenolic compounds are the most abundant natural antioxidants. 3,4 Phenolics can act as antioxidants in many ways.…”

Section: Introductionmentioning

confidence: 99%

“…This eventually leads to chronic diseases such as atherosclerosis, cancer, diabetes, aging and other degenerative diseases. 6 Erythrocytes have been used as a model to investigate oxidative damage due to their high concentration of O 2 and their known sensitivity to endogenous reactive oxygen species (ROS), especially to peroxyl radicals (ROO • ) that may attack membrane components, inducing changes in membrane rheology, conformation of membrane proteins, cellular morphology, protein cross-linking, and hemolysis. 7 Santolina chamaecyparissus L. (Asteraceae) is a shrub with yellow inflorescences that is widely used in Mediterranean folk medicine.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Djarmouni¹,

Baghiani²,

Adjadj³

et al. 2018

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Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH• and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects...

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In vitro and in vivo antioxidant properties and DNA damage protective activity of green fruit of Ficus glomerata

Cited by 57 publications

References 26 publications

Evaluation of antioxidant and free-radical scavenging potential ofArtemisia absinthium

Evaluation of antioxidant and free-radical scavenging potential ofArtemisia absinthium

Phytochemical Screening and ln-vitro Antioxidant Profiling of Solvent Fractions of Canna edulis Ker Gawler

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

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