In Vitro and In Vivo Osteogenic Activity of Titanium Implants Coated by Pulsed Laser Deposition with a Thin Film of Fluoridated Hydroxyapatite

Chen, Luyuan; Komasa, Satoshi; Hashimoto, Yoshiya; Hontsu, Shigeki; Okazaki, Joji

doi:10.3390/ijms19041127

Cited by 32 publications

(31 citation statements)

References 53 publications

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“…Chen et al [180] synthesized using PLD fluoridated synthetic HA (HA:F) structures onto Ti implants and inserted them into rat femurs. By using microcomputed tomography, histology, and sequential polychromatic fluorescent investigations, their osteo-inductive and osseointegration activity in comparison with that of uncoated Ti implants was assessed.…”

Section: In Vivo Testsmentioning

confidence: 99%

Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Duta

Popescu

2019

Coatings

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The aim of this paper is to present the current status on animal-origin hydroxyapatite (HA) coatings synthesized by Pulsed Laser Deposition (PLD) technique for medical implant applications. PLD as a thin film synthesis method, although limited in terms of surface covered area, still gathers interest among researchers due to its advantages such as stoichiometric transfer, thickness control, film adherence, and relatively simple experimental set-up. While animal-origin HA synthesized by bacteria or extracted from animal bones, eggshells, and clams was tested in the form of thin films or scaffolds as a bioactive agent before, the reported results on PLD coatings from HA materials extracted from natural sources were not gathered and compared until the present study. Since natural apatite contains trace elements and new functional groups, such as CO32− and HPO42− in its complex molecules, physical-chemical results on the transfer of animal-origin HA by PLD are extremely interesting due to the stoichiometric transfer possibilities of this technique. The points of interest of this paper are the origin of HA from various sustainable resources, the extraction methods employed, the supplemental functional groups, and ions present in animal-origin HA targets and coatings as compared to synthetic HA, the coatings’ morphology function of the type of HA, and the structure and crystalline status after deposition (where properties were superior to synthetic HA), and the influence of various dopants on these properties. The most interesting studies published in the last decade in scientific literature were compared and morphological, elemental, structural, and mechanical data were compiled and interpreted. The biological response of different types of animal-origin apatites on a variety of cell types was qualitatively assessed by comparing MTS assay data of various studies, where the testing conditions were possible. Antibacterial and antifungal activity of some doped animal-origin HA coatings was also discussed.

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Section: In Vivo Testsmentioning

confidence: 99%

Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Duta

Popescu

2019

Coatings

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“…It is known that regeneration of bone tissue after implantation of any structures depends on the coating on its surface 1,2 . According to research results of F. E. Pinottiet al 3 , the hydrophilic surface implants improve osteointegration in the tibia of rats.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of biochemical markers of the connective tissue metabolism in the blood of rats after insertion of steel implants with diamond-like carbon coating into the femur

Makarov

Морозенко

Gliebova

et al. 2021

Bangladesh J Med Sci

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Objective: It is known that the regeneration of bone tissue after implantation of any structuresdepends on the coating of their surface. Therefore, the research of the influence of steelimplants with diamond-like carbon coating on regeneration of bone tissue in the dynamics afterimplantation is a topical issue. Material and Methods: The study was conducted on 61 male rats,5 of them were intact animals. There were also 2 groups of rats with 28 animals in each group(the 1st group was the control one where implants were without diamond-like carbon coating,the 2nd group was experimental where the implants were without coating). We determined thecontent of glycoproteins, chondroitin sulfates, alkaline phosphatase, total calcium, oxyprolineand osteocalcin in the blood of rats on the 7th, 14th, 30th and 90th days after implantation. Resultsand Discussion: Group 2 rats had a faster osteointegration compared with the group 1 rats, whichwas manifested by the normalization of biochemical markers of bone metabolism (glycoproteins,chondroitinsulfats, oxyproline and osteocalcin) on the 90th day of an experiment. Conclusion:Using steel implants with diamond-like carbon coatings showed that the content of glycoproteinsand chondroitin sulfates increased only on the 7th day; oxyproline and osteocalcin increasedon the 7th, 14th and 30th days after implantation; on the 90th day the rates of these biochemicalmarkers reached the norm, indicating a high efficiency of osteointegration. Bangladesh Journal of Medical Science Vol.20(1) 2021 p.81-85

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“…Currently, huge interest is focused on finding appropriate deposition techniques to modify the surface of metallic implants, by functionalization with coatings, proteins and/or drugs that could favor the enhancement of cellular adhesion, thus leading to the acceleration of the osseointegration time [24][25][26][27]. In the field of thin-film growth, pulsed laser deposition (PLD) has been established in the past 15-20 years as one of the most popular and efficient methods for the deposition of a wide spectrum of materials, especially onto substrates with complex geometrical shapes, including implants [28]. Moreover, when CaPs of animal origin are involved, the PLD technique could be one of the most suitable choices to synthesize this type of coating.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Assessment of Bone Enhancement in the Case of 3D-Printed Implants Functionalized with Lithium-Doped Biological-Derived Hydroxyapatite Coatings: A Preliminary Study on Rabbits

et al. 2020

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We report on biological-derived hydroxyapatite (HA, of animal bone origin) doped with lithium carbonate (Li-C) and phosphate (Li-P) coatings synthesized by pulsed laser deposition (PLD) onto Ti6Al4V implants, fabricated by the additive manufacturing (AM) technique. After being previously validated by in vitro cytotoxicity tests, the Li-C and Li-P coatings synthesized onto 3D Ti implants were preliminarily investigated in vivo, by insertion into rabbits’ femoral condyles. The in vivo experimental model for testing the extraction force of 3D metallic implants was used for this study. After four and nine weeks of implantation, all structures were mechanically removed from bones, by tensile pull-out tests, and coatings’ surfaces were investigated by scanning electron microscopy. The inferred values of the extraction force corresponding to functionalized 3D implants were compared with controls. The obtained results demonstrated significant and highly significant improvement of functionalized implants’ attachment to bone (p-values ≤0.05 and ≤0.00001), with respect to controls. The correct placement and a good integration of all 3D-printed Ti implants into the surrounding bone was demonstrated by performing computed tomography scans. This is the first report in the dedicated literature on the in vivo assessment of Li-C and Li-P coatings synthesized by PLD onto Ti implants fabricated by the AM technique. Their improved mechanical characteristics, along with a low fabrication cost from natural, sustainable resources, should recommend lithium-doped biological-derived materials as viable substitutes of synthetic HA for the fabrication of a new generation of metallic implant coatings.

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In Vitro and In Vivo Osteogenic Activity of Titanium Implants Coated by Pulsed Laser Deposition with a Thin Film of Fluoridated Hydroxyapatite

Cited by 32 publications

References 53 publications

Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources

Dynamics of biochemical markers of the connective tissue metabolism in the blood of rats after insertion of steel implants with diamond-like carbon coating into the femur

In Vivo Assessment of Bone Enhancement in the Case of 3D-Printed Implants Functionalized with Lithium-Doped Biological-Derived Hydroxyapatite Coatings: A Preliminary Study on Rabbits

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