In vitro and in vivo antioxidant activity of the ethanolic extract from Meconopsis quintuplinervia

He, Jingsheng; Huang, Bo; Ban, Xiaoquan; Tian, Jun; Zhu, Ling; Wang, Youwei

doi:10.1016/j.jep.2012.02.006

Cited by 54 publications

(28 citation statements)

References 29 publications

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“…Gallic acid, ascorbic acid, and BHT were used as standard compound. The percentage of the DPPH radical inhibition was calculated using the following equation [17]: % inhibition= [(Abscontrol -Abssample)/ Abscontrol ] x100…”

Section: Dpph Test For Antioxidant Activitymentioning

confidence: 99%

Antioxidant Capacity and Metal Content of Physalis peruviana L. Fruit Sold in Markets

Eken¹,

Ünlü-Endirlik²,

Baldemir³

et al. 2016

Ann Clin Anal Med

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ÖzetAmaç: Antioksidan aktivite gösteren ve eser elementlerin gerekli miktarları-nı içeren tıbbi gıdaların diyetle alınması, iyi bir sağlıklı yaşam sürdürmek için önemlidir. Çalışmamızda, Kayseri'de marketlerde satılan Altınçilek (Physalis peruviana L.) meyvesinin antioksidan kapasitesinin ve metal içeriğinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: P. peruviana meyvesinin antioksidan kapasitesi, 2,2-difenil-1-pikrilhidrazil (DPPH) serbest radikal süpürme testi ile belirlenmiştir. Meyvenin metanol (MeOH) ekstresinin DPPH serbest radikalini temizleme kapasitesi, referans olarak askorbik asit, gallik asit ve bütillen-dirilmiş hidroksitoluen (BHT) gibi bilinen antioksidanlarla karşılaştırılmıştır. P. peruviana meyvesinin metal içeriği atomik absorpsiyon spektrometresi (AAS) kullanılarak ölçülmüştür. Bulgular: P. peruviana meyve ekstresinin DPPH serbest radikal temizleme aktivitesinin olduğu, fakat antioksidan kapasitesinin standart maddelerden daha düşük olduğu tespit edilmiştir. P. peruviana, askorbik asit, gallik asit ve BHT'nin % 50 inhibitör konsantrasyon (IC 50 ) değer-leri sırası ile 32 mg/ml, 3.8 mg/ml, 3.51 mg/ml ve 1.21 mg/ml olarak belirlenmiştir. AAS ile yapılan analizler sonucu, meyvenin eser element yönünden zengin içeriğe sahip iken ağır metal içeriğinin az miktarda veya hiç olmadı-ğı gözlenmiştir. Tartışma: Bu sonuçlar, P. peruviana meyvesinin doğal kaynaklı potansiyel bir antioksidan ve eser element kaynağı olduğunu göstermektedir. Anahtar Kelimeler Eser Elementler; Physalis; Antioksidan EtkiAbstract Aim: The dietary intake of medicinal food with antioxidant activity and required amounts of trace elements is important to pursue good healthy life. In our study, we aimed to determine the antioxidant capacity and metal content of goldenberry (Physalis peruviana L.) fruit sold in markets in Kayseri. Material and Method: The antioxidant capacity of P. peruviana fruit was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. The DPPH radical scavenger capacity of the methanol extract of fruit was compared with known antioxidants such as ascorbic acid, gallic acid, and butylated hydroxytoluene (BHT) as references. The metal content of P. peruviana fruit was measured by using atomic absorption spectrometer (AAS). Results: The fruit of P. peruviana was found to possess DPPH free radical scavenging activity but the antioxidant capacity was lower than the standard substances. Inhibitory concentration 50% (IC 50 ) values of P. peruviana, ascorbic acid, gallic acid, and BHT were determined as 32 mg/ml, 3.8 mg/ml, 3.51 mg/ ml, and 1.21 mg/ml, respectively. As a result of the analysis by AAS, it was observed that P. peruviana fruit contented plentiful trace elements and the content of heavy metal was small amount or not detected. Discussion: These observations suggest that the fruit of P. peruviana has a potential source of antioxidant and trace elements of natural origin.

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Section: Dpph Test For Antioxidant Activitymentioning

confidence: 99%

Antioxidant Capacity and Metal Content of Physalis peruviana L. Fruit Sold in Markets

Eken¹,

Ünlü-Endirlik²,

Baldemir³

et al. 2016

Ann Clin Anal Med

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“…The MDA level was then normalized to milligram protein, as previously described [12] . SOD is one of the most important antioxidative enzymes [13] and its activity was measured with a colorimetric assay kit according to the manufacturer's protocol. Briefly, SOD activity was measured using 100 μg of the total protein extract from the emodin-treated human T cells.…”

Section: Malondialdehyde and Total Superoxide Dismutase Assaymentioning

confidence: 99%

Emodin induces human T cell apoptosis in vitro by ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction

Shen

et al. 2013

Acta Pharmacol Sin

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Aim: To elucidate the molecular mechanisms underlying the immunosuppressive effects of emodin isolated from Rheum palmatum L. Methods: Human T cells were isolated from the peripheral venous blood of 10 healthy adult donors. Cell viability was analyzed with MTT assay. AO/EB and Annexin V/PI staining and DNA damage assay were used to detect cell apoptosis. Fluorescence staining was used to detect the levels of ROS, the mitochondrial membrane potential and intracellular Ca 2+ . Colorimetry was used to detect the levels of MDA and total SOD and GSH/GSSG ratio. The expression and activity of caspase-3, -4, and -9 were detected with Western blotting and a fluorometric assay. Western blotting was also used to detect the expression of Bcl-2, Bax, cytochrome C, and endoplasmic reticulum (ER) markers. Results: Emodin (1, 10, and 100 µmol/L) inhibited the growth of human T cells and induced apoptosis in dose-and time-dependent manners. Emodin triggered ER stress and significantly elevated intracellular free Ca 2+ in human T cells. It also disrupted mitochondrial membrane potential, and increased cytosolic level of cytochrome C, and the levels of activated cleavage fragments of caspase-3, -4, and -9 in human T cells. Furthermore, emodin significantly increased the levels of ROS and MDA, inhibited both SOD level and GSH/ GSSG ratio in human T cells, whereas co-incubation with the ROS scavenger N-acetylcysteine (NAC, 20 µmol/L) almost completely blocked emodin-induced ER stress and mitochondrial dysfunction in human T cells, and decreased the caspase cascade-mediated apoptosis. Conclusion: Emodin exerts immunosuppressive actions at least partly by inducing apoptosis of human T cells, which is triggered by ROS-mediated ER stress and mitochondrial dysfunction.

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“…Like MDA levels, CAT measured in tissues is indicative of the degree of damages the tissues are undergoing or the degree of protection offered by the protective enzymatic agents against ROS. Significantly reversed CAT, GSH and MDA levels were recorded for CCl 4 -induced oxidative stress in mice treated with ethanolic extract of Meconopsis quintuplinervia [21] . In the present study, the leaf extracts of D. edulis and F. exasperata showed significantly higher CAT levels in blood and liver tissues in experimental rats.…”

Section: Discussionmentioning

confidence: 90%

Assessment of In vivo antioxidant properties of Dacryodes edulis and Ficus exasperata as anti-malaria plants

Conrad

Uche

2013

Asian Pacific Journal of Tropical Disease

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In vitro and in vivo antioxidant activity of the ethanolic extract from Meconopsis quintuplinervia

Cited by 54 publications

References 29 publications

Antioxidant Capacity and Metal Content of Physalis peruviana L. Fruit Sold in Markets

Antioxidant Capacity and Metal Content of Physalis peruviana L. Fruit Sold in Markets

Emodin induces human T cell apoptosis in vitro by ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction

Assessment of In vivo antioxidant properties of Dacryodes edulis and Ficus exasperata as anti-malaria plants

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