In vitro and in vivo investigation for biological activities of neoagarooligosaccharides prepared by hydrolyzing agar with β-agarase

Hong, Sang Jeen; Lee, Je-Hyeon; Kim, Eun Joo; Yang, Hea Jung; Chang, YongKeun; Park, Jae-Seon; Hong, Soon-Kwang

doi:10.1007/s12257-017-0049-8

Cited by 38 publications

(20 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AOS has specic physiological functions, such as antioxidant, 19 prebiotic, 20 anti-inammatory, 21 and antimicrobial activity, glucosidase inhibition and melanin biosynthesis. 22 In particular, AOS has high water solubility, is easily absorbed by the body, and contains a large number of free hydroxyl groups, 23,24 which may coordinate with metal ions. Hence, AOS is a suitable choice as a ligand for chelating iron.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of AOS–iron on iron deficiency anemia in rats

Huang

Liu

et al. 2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Effectiveness of AOS–iron on iron deficiency anemia in rats

Huang

Liu

et al. 2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…NA4 and NA6 harbor many biological activities, and the safety of NA4 and NA6 has been confirmed by previous genotoxicity test [36]. Moreover, NA4 and NA6 exhibit antibacterial activity against several Gram-negative and -positive bacteria [37]. Wang et al demonstrated that NAs with different degrees of polymerization, including NA4 and NA6, possess anti-inflammation activity in LPS-stimulated macrophages [38].…”

Section: Discussionmentioning

confidence: 86%

Extracellular expression of agarase rAgaM1 in Bacillus subtilis and its ability for neoagaro‐oligosaccharide production

Jin

et al. 2019

J Basic Microbiol

View full text Add to dashboard Cite

An agarase gene (agaM1) was cloned, expressed and characterized by using Escherichia coli as host strain, revealing the outstanding properties of recombinant AgaM1 (rAgaM1) in agarose degradation and neoagaro‐oligosaccharides (NAs) production in our previous work. In current study, agaM1 was extracellularly expressed in Bacillus subtilis, and we aim to assess the ability of the supernatant of recombinant B. subtilis fermentation broth containing rAgaM1 to degrade agarose without protein purification, which would save the cost of purification and avoid the activity loss during purification. The pH and temperature optima for the supernatant were 7.0 and 50 °C, respectively. The supernatant containing rAgaM1 has outstanding stability against 40 °C and 50 °C. Besides, we detailedly studied the possible influence factors of rAgaM1 expression in the supernatant, including pH, temperature, isopropyl β‐D‐thiogalactoside (IPTG) concentration, initial optical density at a wavelength of 600 nm (OD600), and induction time, and the optimum conditions for rAgaM1 expression by B. subtilis were confirmed. Moreover, the supernatant was able to produce NAs by using the Gracilaria lemaneiformis, whose cells were broken by autoclaving, as substrate, and a total of 1.41 µmol ml−1 of NA, including neoagarotetraose and neoagarohexaose, was produced after degradation for 48 h. This ability could save the cost of substrates in NA production, although the method requires a further study. Our results reveal that the NAs with great potential in food and pharmaceutical industries could be inexpensive to make by the supernatant containing rAgaM1 of B. subtilis fermentation broth in the foreseeable future.

show abstract

“…Inhibitor kinetic studies of Ru were done like those used in a kinetic study for mutansucrase, except that multiple concentrations (0- 2.4. Antibacterial effect assay by disc diffusion method S. mutans was cultured in brain-heart infusion (BHI) at 37 o C until OD 600 reached approximately 1.0 [13,14]. The antibacterial effect of Ru solution (250 mM) against S. mutans was measured using epigallocatechin gallate (EGCG) (250 mM) as a control [15].…”

Section: Inhibition Activity Of Rubusoside Against Mutansucrasementioning

confidence: 99%

Anti-cariogenic Characteristics of Rubusoside

Kim

Nguyen

Jin

et al. 2019

Biotechnol Bioproc E

View full text Add to dashboard Cite

Streptococcus mutans plays an important role in the development of dental caries in humans by synthesizing adhesive insoluble glucans from sucrose by mutansucrase activity. To explore the anti-cariogenic characteristics of rubusoside (Ru), a natural sweetener component in Rubus suavissimus S. Lee (Rosaceae), we investigated the inhibitory effect of Ru against the activity of mutansucrase and the growth of Streptococcus mutans. Ru (50 mM) showed 97% inhibitory activity against 0.1 U/mL mutansucrase of S. mutans with 500 mM sucrose. It showed competitive inhibition with a K i value of 1.1 ± 0.2 mM and IC 50 of 2.3 mM. Its inhibition activity was due to hydrophobic and hydrogen bonding interactions based on molecular docking analysis. Ru inhibited the growth of S. mutans as a bacteriostatic agent, with MIC and MBC values of 6 mM and 8 mM, respectively. In addition, Ru showed synergistic anti-bacterial activity when it was combined with curcumin. Therefore, Ru is a natural anti-cariogenic agent with antimutansucrase activity and antimicrobial activity against S. mutans.

show abstract

In vitro and in vivo investigation for biological activities of neoagarooligosaccharides prepared by hydrolyzing agar with β-agarase

Cited by 38 publications

References 15 publications

Effectiveness of AOS–iron on iron deficiency anemia in rats

Effectiveness of AOS–iron on iron deficiency anemia in rats

Extracellular expression of agarase rAgaM1 in Bacillus subtilis and its ability for neoagaro‐oligosaccharide production

Anti-cariogenic Characteristics of Rubusoside

Contact Info

Product

Resources

About