In vitro and in vivo assessment of a <scp>3D</scp> printable gelatin methacrylate hydrogel for bone regeneration applications

Celikkin, Nehar; Mastrogiacomo, Simone; Dou, Weiqiang; Heerschap, Arend; Oosterwijk, Egbert; Walboomers, X. Frank; Święszkowski, Wojciech

doi:10.1002/jbm.b.35067

Cited by 20 publications

(15 citation statements)

References 64 publications

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“…It is encouraging that the η * (figure 2(C)) and G ′ (figure 2(D)) of the bioinks were inferior (below 100 Pa s and 0.5 kPa, respectively) at cartridge temperature (about 20 • C) and very high (above 750 Pa s and 5 kPa, respectively) at platform temperature (10 • C), which were favorable for bioinks extrusion printing from the needle and scaffold accelerating molding on the platform. The phenomenon was similar to the thermosensitive bioinks in recent studies [16,18,63]. In addition, due to the increase of nHAp content, the relative concentration of Gel decreased, which resulted in the pre-gel temperature of dGQH bioinks decreasing compared to dGQ bioink (figure 2(E)).…”

Section: Synthesis and Rheological Properties Of The Bioinkssupporting

confidence: 84%

3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis

Kang¹,

Xu²,

Meng³

et al. 2023

Biofabrication

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Craniofacial bone regeneration is a coupled process of angiogenesis and osteogenesis, which, associated with infection, still remains a challenge in bone defects after trauma or tumor resection. 3D tissue engineering scaffolds with multifunctional-therapeutic properties can offer many advantages for the angiogenesis and osteogenesis of infected bone defects. Hence, in the present study, a microchannel networks-enriched 3D hybrid scaffold composed of decellularized extracellular matrix (dECM), gelatin (Gel), quaterinized chitosan (QCS) and nano-hydroxyapatite (nHAp) (dGQH) was fabricated by an extrusion 3D bioprinting technology. And enlightened by the characteristics of natural bone microstructure and the demands of vascularized bone regeneration, the exosomes (Exos) isolated from human adipose derived stem cells as angiogenic and osteogenic factors were then co-loaded into the desired dGQH20 hybrid scaffold based on an electrostatic interaction. The results of the hybrid scaffolds performance characterization showed that these hybrid scaffolds exhibited an interconnected pore structure and appropriate degradability (>61% after 8 weeks of treatment), and the dGQH20 hybrid scaffold displayed the highest porosity (83.93 ± 7.38%) and mechanical properties (tensile modulus: 62.68 ± 10.29 MPa, compressive modulus: 16.22 ± 3.61 MPa) among the dGQH hybrid scaffolds. Moreover, the dGQH20 hybrid scaffold presented good antibacterial activities (against 94.90 ± 2.44% of Escherichia coli and 95.41 ± 2.65% of Staphylococcus aureus, respectively) as well as excellent hemocompatibility and biocompatibility. Furthermore, the results of applying the Exos to the dGQH20 hybrid scaffold showed that the Exo promoted the cell attachment and proliferation on the scaffold, and also showed a significant increase in osteogenesis and vascularity regeneration in the dGQH@Exo scaffolds in vitro and in vivo. Overall, this novel dECM/Gel/QCS/nHAp hybrid scaffold laden with Exo has a considerable potential application in reservation of craniofacial bone defects.

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Section: Synthesis and Rheological Properties Of The Bioinkssupporting

confidence: 84%

3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis

Kang¹,

Xu²,

Meng³

et al. 2023

Biofabrication

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“…Moreover, the performance of 3D printed gelatin methacrylate hydrogel has formerly been investigated after implantation in rat condyle defects. Whereas optimal tissue integration was observed via histology, no signs of fibrotic encapsulation or inhibited bone formation were attained [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Histological and Histomorphometric Evaluation of Implanted Photodynamic Active Biomaterials for Periodontal Bone Regeneration in an Animal Study

Sigusch

Kranz

Hohenberg

et al. 2023

IJMS

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Recently, our group developed two different polymeric biomaterials with photodynamic antimicrobial surface activity for periodontal bone regeneration. The aim of the present study was to analyze the biocompatibility and osseointegration of these materials in vivo. Two biomaterials based on urethane dimethacrylate (BioM1) and tri-armed oligoester-urethane methacrylate (BioM2) that additionally contained ß-tricalcium phosphate and the photosensitizer mTHPC (meso-tetra(hydroxyphenyl)chlorin) were implanted in non-critical size bone defects in the femur (n = 16) and tibia (n = 8) of eight female domestic sheep. Bone specimens were harvested and histomorphometrically analyzed after 12 months. BioM1 degraded to a lower extent which resulted in a mean remnant square size of 17.4 mm², while 12.2 mm² was estimated for BioM2 (p = 0.007). For BioM1, a total percentage of new formed bone by 30.3% was found which was significant higher compared to BioM2 (8.4%, p < 0.001). Furthermore, BioM1 was afflicted by significant lower soft tissue formation (3.3%) as compared to BioM2 (29.5%). Additionally, a bone-to-biomaterial ratio of 81.9% was detected for BioM1, while 8.5% was recorded for BioM2. Implantation of BioM2 caused accumulation of inflammatory cells and led to fibrous encapsulation. BioM1 (photosensitizer-armed urethane dimethacrylate) showed favorable regenerative characteristics and can be recommended for further studies.

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“…The study showed that gelMA and MSCs together have enhanced ability for the differentiation of the MSCs due to the porous nature of the gelMA. The MSCs that were encapsulated with the gelMA showed potential for future BTE experiments [77]. Beyond scaffolds, 3D printing can be used for a wide array of bone repair applications, including screws, plates, and gels [32].…”

Section: Hydrogels In 3d Bioprinting and Where To Improvementioning

confidence: 99%

Three-Dimensional Bioprinting Applications for Bone Tissue Engineering

Maresca

DeMel

Wagner

et al. 2023

Cells

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The skeletal system is a key support structure within the body. Bones have unique abilities to grow and regenerate after injury. Some injuries or degeneration of the tissues cannot rebound and must be repaired by the implantation of foreign objects following injury or disease. This process is invasive and does not always improve the quality of life of the patient. New techniques have arisen that can improve bone replacement or repair. 3D bioprinting employs a printer capable of printing biological materials in multiple directions. 3D bioprinting potentially requires multiple steps and additional support structures, which may include the use of hydrogels for scaffolding. In this review, we discuss normal bone physiology and pathophysiology and how bioprinting can be adapted to further the field of bone tissue engineering.

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In vitro and in vivo assessment of a 3D printable gelatin methacrylate hydrogel for bone regeneration applications

Cited by 20 publications

References 64 publications

3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis

3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis

Histological and Histomorphometric Evaluation of Implanted Photodynamic Active Biomaterials for Periodontal Bone Regeneration in an Animal Study

Three-Dimensional Bioprinting Applications for Bone Tissue Engineering

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