In vitro and in silico analyses reveal the interaction between LysM receptor‐like kinase3 of Solanum tuberosum and the carbohydrate elicitor Riclin octaose
Hongyang Wang,
Ruixin Zhang,
Junpeng Hu
et al.
Abstract:As a carbohydrate elicitor, Riclin octaose (Rioc) activates the pattern‐triggered immunity of Solanum tuberosum L., while how the plant perceives Rioc is unknown. Here, a pattern recognition receptor StLYK3 (LysM receptor‐like kinase3) whose transcription level was significantly up‐regulated after Rioc elicitation was investigated in vitro and in silico. The nucleotide that encoded the ectodomain of StLYK3 (StLYK3‐ECD) was heterologously expressed in the Pichia pastoris strain GS115. The purified StLYK3‐ECD ha… Show more
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