2015
DOI: 10.1590/1516-635x1704427-432
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Abstract: This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combinati… Show more

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Cited by 5 publications
(20 citation statements)
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References 30 publications
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“…During the infection process, Salmonella will make contact with the host cell by attaching to the host cell through their receptors, and the level of adhesion and invasion are the very first virulence determinants for successful infection. 25,26 The adhesion and invasion studies conducted on non-phagocytic HeLa, HepG2 and Caco-2 cells revealed the lon deletion significantly increased (approximately 2-fold increase) both adhesion and invasion into the host cell. This was confirmed by CFU counting and by fluorescence microscopy.…”
Section: Discussionmentioning
confidence: 99%
“…In addition, the vaccination programme is stricter in the Valencia region where, since 2008, it is mandatory to vaccinate not only against S. Enteritidis, but also against S. Typhimurium [19]. Live vaccination in poultry maintains the Salmonella vaccine strain in birds, as well as the house environment [34][35][36], and could encourage phage presence in the field. In this context, the latest data recovered from official checks in the Valencia region showed that 100% of S. Enteritidis strains isolated from rearing layers were S. Enteritidis vaccine strains (unpublished data).…”
Section: Discussionmentioning
confidence: 99%
“…We were also able to examine the viability of the eukaryotic cells and the integrity of the epithelial barrier in the presence and absence of phage. A number of recent studies have used in vitro cell culture models to test the behavior of phage in the gut and their activity against intestinal pathogens [ 60 , 61 ]. To date, most have used single cell monolayers without a mucus layer [ 60 , 61 ].…”
Section: Discussionmentioning
confidence: 99%
“…A number of recent studies have used in vitro cell culture models to test the behavior of phage in the gut and their activity against intestinal pathogens [ 60 , 61 ]. To date, most have used single cell monolayers without a mucus layer [ 60 , 61 ]. More complex models using co-cultures have been utilized for the interaction of the phage with the epithelial layer.…”
Section: Discussionmentioning
confidence: 99%