In situ thermal gelling polypeptide for chondrocytes 3D culture

Choi, Bo Gyu; Park, Min Hee; Cho, So-Hye; Joo, Min Kyung; Oh, Hyo-Jung; Kim, Eun Hye; Han, Dong Keun; Jeong, Byeongmoon

doi:10.1016/j.biomaterials.2010.08.067

Cited by 93 publications

(86 citation statements)

References 31 publications

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“…63 (sGAG) and collagen type II. 31 The modulus of the hydrogel significantly affects the proliferation and differentiation of cells. 64,65 The modulus could be controlled by varying the initial concentration of (DL/L)-PA-PLX-(DL/L)-PA. 66 Live cell microscopy is an interesting application for the thermogel because the cells are captured in the transparent gel with an appropriate modulus during the sol-to-gel transition.…”

Section: How To Shorten the Dissolution Timementioning

confidence: 99%

Biodegradable Thermogels

et al. 2011

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All living creatures respond to external stimuli. Similarly, some polymers undergo conformational changes in response to changes in temperature, pH, magnetic field, electrical field, or the wavelength of light. In one type of stimuli-responsive polymer, thermogel polymers, the polymer aqueous solution undergoes sol-to-gel transition as the temperature increases. Drugs or cells can be mixed into the polymer aqueous solution when it is in its lower viscosity solution state. After injection of the solution into a target site, heating prompts the formation of a hydrogel depot in situ, which can then act as a drug releasing system or a cell growing matrix. In this Account, we describe key materials developed in our laboratory for the construction of biodegradable thermogels. We particularly emphasize recently developed polypeptide-based materials where the secondary structure and nanoassembly play an important role in the determining the material properties. This Account will provide insights for controlling parameters, such as the sol-gel transition temperature, gel modulus, critical gel concentration, and degradability of the polymer, when designing a new thermogel system for a specific biomedical application. By varying the stereochemistry of amino acids in polypeptides, the molecular weight of hydrophobic/hydrophilic blocks, the composition of the polypeptides, the hydrophobic end-capping of the polypeptides, and the microsequences of a block copolymer, we have controlled the thermosensitivity and nanoassembly patterns of the polymers. We have investigated a series of thermogel biodegradable polymers. Polymers such as poly(lactic acid-co-glycolic acid), polycaprolactone, poly(trimethylene carbonate), polycyanoacrylate, sebacic ester, polypeptide were used as hydrophobic blocks, and poly(ethylene glycol) and poly(vinyl pyrrolidone) were used as hydrophilic blocks. To prepare a polymer sensitive to pH and temperature, carboxylic acid or amine groups were introduced along the polymer backbone. The sol-gel transition mechanism involves changes in the secondary structures of the hydrophobic polypeptide and in the conformation of the hydrophilic block. The polypeptide copolymers were stable in the phosphate buffered saline, but the presence of proteolytic enzymes such as elastase, cathepsin B, cathepsin C, and matrix metallopreoteinase accelerated their degradation. We also describe several biomedical applications of biogradable thermogel polymers. One subcutaneous injection of the insulin formulation of thermogel polypeptide copolymers in diabetic rats provided hypoglycemic efficacy for more than 16 days. The thermogels also provided a compatible microenvironment for chondrocytes, and these cells produced biomarkers for articular cartilage such as sulfated glucoaminoglycan (sGAG) and type II collagen. The thermogels were also used as a fixing agent for in situ cell imaging, and cellular activities such as endocytosis were observed by live cell microscopy.

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Section: How To Shorten the Dissolution Timementioning

confidence: 99%

Biodegradable Thermogels

et al. 2011

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“…Polyalanine-poloxamer-polyalanine (PA-PLX-PA) block copolymers have been developed for this kind of application [100]. Specifically hybrids were constituted by a segment where a central hydrophilic moiety of poly(ethylene oxide) was flanked by two hydrophobic moieties of poly(propylene oxide).…”

Section: Crosslinked Hydrogels and Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Hybrid Block Copolymers Constituted by Peptides and Synthetic Polymers: An Overview of Synthetic Approaches, Supramolecular Behavior and Potential Applications

Morell

Puiggalı́

2013

Polymers

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Hybrid block copolymers based on peptides and synthetic polymers, displaying different types of topologies, offer new possibilities to integrate the properties and functions of biomacromolecules and synthetic polymers in a single hybrid material. This review provides a current status report of the field concerning peptide-synthetic polymer hybrids. The first section is focused on the different synthetic approaches that have been used within the last three years for the preparation of peptide-polymer hybrids having different topologies. In the last two sections, the attractive properties, displayed in solution or in the solid state, together with the potential applications of this type of macromolecules or supramolecular systems are highlighted.

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“…(L/DL)-PA-Pluronic ® -(L/DL)-PA thermogel showed a higher population of nanofibrous structures as the L-Ala content increased and chondrocytes cultured in these thermogels expressed higher chondrogenic markers compared to commercially available Matrigel TM (Fig. 15) [3]. For the successful tissue regeneration, we need to better understand cell-matrix interactions.…”

Section: Biomedical Applicationsmentioning

confidence: 97%

“…These nano-assemblies can give unique nanostructures in the hydrogels. Thus, polypeptide-based hydrogels can provide biomimetic ECMs with various nanostructures that can affect proliferation and/or differentiation of encapsulated cells [3,63].…”

Section: Chemical Hydrogelsmentioning

confidence: 99%

“…Wichterle and Lim [2] reported the pioneering work using hydrophilic 2-hydroxyethyl methacrylate (HEMA) hydrogels in 1960. Recently, in situ gelling systems based on various natural and synthetic polymers have been widely investigated for biomedical applications due to effective encapsulation of cells and bioactive molecules, minimally invasive injection, and easy formation in any desired shape of defects, in addition to several advantages of typical hydrogels including high water contents similar to extracellular matrix (ECM), controllable physicochemical properties, and efficient mass transfer [3][4][5]. When hydrogels are prepared by covalent-crosslinking, they form permanent or chemical gels.…”

Section: Introductionmentioning

confidence: 99%

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Introduction to In Situ Forming Hydrogels for Biomedical Applications

Choi

Loh

Tan

et al. 2014

In-Situ Gelling Polymers

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In situ gelling polymer aqueous solutions undergo sol-to-gel transition through chemical and/or physical crosslinking. The criterion on sol and gel is an important issue, therefore, rheology of hydrogel have been discussed in detail. The in situ gelling system has been investigated for minimally invasive drug delivery, injectable tissue engineering, gene delivery, and wound healing. In this chapter, in situ gelling systems and their various biomedical applications were briefly summarized.

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In situ thermal gelling polypeptide for chondrocytes 3D culture

Cited by 93 publications

References 31 publications

Biodegradable Thermogels

Biodegradable Thermogels

Hybrid Block Copolymers Constituted by Peptides and Synthetic Polymers: An Overview of Synthetic Approaches, Supramolecular Behavior and Potential Applications

Introduction to In Situ Forming Hydrogels for Biomedical Applications

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