In situ synthesis of hydroxyapatite nanocomposites using iron oxide nanofluids at ambient conditions

Abstract

This paper describes a simple method for the room temperature synthesis of magnetite/hydroxyapatite composite nanocomposites using ferrofluids. The in situ synthesis of magnetic-hydroxyapatite results in a homogenous distribution of the two phases as seen both in transmission electron micrographs and assembled to a micron range in the confocal micrographs. The selected area diffraction pattern analysis shows the presence of both phases of iron oxide and hydroxyapatite. To the dialyzed ferrofluid, the constituents of hydroxyapatite synthesis was added, the presence of the superparamagnetic iron oxide particles imparts directionality to the hydroxyapatite crystal growth. Electron probe microanalysis confirms the co-existence of both iron and calcium atoms. Vibrating Sample magnetometer data shows magnetization three times more than the parent ferrofluid, the local concentration of iron oxide nanoparticles affects the strength of dipolar interparticle interactions changing the energy barrier for determining the collective magnetic behavior of the sample. The limitations inherent to the use of external magnetic fields which can be circumvented by the introduction of internal magnets located in the proximity of the target by a minimal surgery or by using a superparamagnetic scaffold under the influence of externally applied magnetic field inspires us to increase the magnetization of our samples. The composite in addition shows anti-bacterial properties against the two gram (-ve) bacteria tested. This work is significant as magnetite-hydroxyapatite composites are attracting a lot of attention as adsorbents, catalysts, hyperthermia agents and even as regenerative medicine.

Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery

“…The peak at 1037 cm -1 is associated with the stretching modes of the P-O bonds of HA and the doublet at 606 cm -1 and 560 cm -1 are due to bending modes of P-O bonds in phosphate group further confirming the presence of phosphate in the system [51]. Three well defined strong peaks at 1380 cm -1 , 1645 cm -1 and 3154 cm -1 are assigned to C-N, C-O and the stretching of N-H bond respectively seen only in C-HA and B-HA due to the presence of proteins/amino acids [52]. Presence of CO 3 2group is revealed by a weak peak at 870 cm -1 in case of Bare-HA.…”

“…51 Three well-dened strong peaks at 1380 cm À1 , 1645 cm À1 and 3154 cm À1 are assigned to C-N, C-O and stretching of the N-H bond respectively, and are seen only in C-HA and B-HA due to the presence of proteins/amino acids. 52 Presence of the CO 3 2À group is revealed by a weak peak at 870 cm À1 in the case of bare-HA. To further cross-check our FT-IR results we have characterized our samples by the XPS technique, which will conclude the chemical linkage of different species present in the sample, and most importantly the interaction of the matrix with HA, which was not visible by FT-IR.…”

Synthesis of Heparin-Immobilized, Magnetically Addressable Cellulose Nanofibers for Biomedical Applications

“…In biomedical applications, the most commonly utilized forms of superparamagnetic iron oxide magnetic NPs, also referred to as SPIONs, are maghemite (γ-Fe 2 O 3 ) and magnetite (Fe 3 O 4 ). , SPIONs are generally well-tolerated in biological systems and are similar in size to biomacromolecules. Therefore, they are ideal substrates to be functionalized with biomolecules to produce nanobioconjugates for biomedical applications.…”

Biomimetically Synthesized Aqueous Ferrofluids Having Antibacterial and Anticancer Properties

“…Although, many contradictory results have been published about the toxicity of superparamagnetic iron oxide nanoparticles, recently [31] reported that the surface coating large influences different cell types and the possible toxic effects vary accordingly [13]. Tartaj et al (2003) [32] [33] reported that the size, shape, and magnetic dipole moment of the particles may also play a role in in vivo experiments. The one pot biomimetic synthesis i.e.…”