In Silico Study of Mirna-Regulated Iq Motif-Containing Gtpase-Activating Protein Family in Liver Cancer

Abstract

Objective: The aim of this paper is to identify the list of microRNA (miRNA) which can regulate the aberrant expression of IQGAP in liver cancer formation. The aberrant expression of IQ motif-containing GTPase-activating protein (IQGAP) family which consists of IQGAP1, IQGAP2, and IQGAP3 has been linked to carcinogenesis in human cancers. The reciprocal expression of IQGAP family in human cancer has been studied to act as oncogenes or tumor suppressor genes. A growing number of studies suggest that upregulated or downregulated expression of IQGAP family triggers cancer development.Methods: A correlation study was performed to construct a pathway to inhibit or activate IQGAP family between miRNAs and IQGAPs. A pre-processing step was conducted to download, filter and process the dataset from TCGA. It yields miRNA and IQGAP gene expression matrix. Then, correlation computation was computed using MATLAB. Moreover, this study linked the results to the MiRTarBase to validate the prediction result with the wet lab experimental result.Results: This study identified significantly inversely correlation in 51 miRNAs-IQGAP1, 169 miRNAs-IQGAP2, and 33 miRNAs-IQGAP3, respectively, which may potentially play a role in a liver cancer formation. Some of the results also can be found in miRTarBase. It supports the precision of those miRNA and IQGAP interaction between dry lab and wet lab study. IQGAP1 and IQGAP2 mostly has been identified as an oncogene in cancer but IQGAP2 has been discovered as tumor suppressor gene. The list of miRNA in the result of this study can become a potential therapy to target the aberrant expression of IQGAP family.Conclusion: miRNA function is known as an oncogene or tumor suppressor gene in cancer development. Therefore, it can be one of the important molecular biology which may target the aberrant expression of IQGAP in liver cancer.

The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro

“…Based on the author҆ s knowledge, there is no experimental publication on the miR-4800 manner in cancer development. As previously described, deregulation of miR-4800 has been indicated in different cancers, such as BC, 7 - 9 cervical cancer, 10 colorectal cancer, 11 , 12 ESCC, gastric cancer, 13 glioma, 14 liver carcinoma, 15 , 19 and pancreatic cancer. Indeed, downregulation of miR-4800 was commonly investigated in patients with these cancers.…”

“…Certain miRNAs, in comparison, show tumor-suppressive properties. 6 In this regard, dysregulation of miR-4800 has been identified in various cancers, such as BC, 7 - 9 cervical cancer, 10 colorectal cancer, 11 , 12 esophageal squamous cell carcinoma (ESCC), stomach cancer, 13 glioma, 14 liver carcinoma, 15 and pancreatic cancer. Indeed, downregulation of miR-4800 was investigated in patients with these cancers.…”

Pharmacogenomics

“…Instances wherein an individual's reactions to specific drugs are encompassed are represented by conditions such as Stevens-Johnson syndrome or epidermal toxic necrolysis, resistance to clopidogrel, malignant hyperthermia, sensitivity and resistance to warfarin, and deficiency in thiopurine S-methyltransferase [23]. While the integration of pharmacogenomics into clinical practice is progressively expanding, a notable absence persists in terms of an established genetic or any other clinical biomarker that could consistently forecast an individual's response to a chosen Multiple Sclerosis (MS) therapy.…”

Pharmacogenomics: Overview, Applications, and Recent Developments

“…This branch is associated with genomics is genetic level studies with functional studies and pharmacology (includes pharmacokinetics and pharmacodynamics). All these branches together aid in the development of safe, and effective medications along with doses which are probably tailored to an individual persona genetic makeup [20][21][22][23][24]. Pharmacogenetics is indicated as major clinically proven Table 2.…”