In silico characterization of putative drug targets in Staphylococcus saprophyticus, causing bovine mastitis

Abstract

The bovine mastitis caused by coagulase negative staphylococci (CNS) has increased in many herds of urban and rural areas of India. Emergence of multi drug resistant bacteria has further made its management more complex and serious. Therefore, innovation of novel specific drug for the treatment of disease caused by particular organism remained to be a challenge. Hence, in the present study a bacterium was isolated from milk of the cow with bovine mastitis and was identified as S. saprophyticus, 44 pathways of S. saprophyticus retrieved (KEGG) from web server were found to be non homologous to the host Bos taurus, out of which 39 pathways were found to be in cytoplasm, 2 in cell wall and 3 in the cell membrane. The knowledge of the present study could make the drug discovery easier which have high affinity to the target site of the causative organism.

Veterinary systems biology for bridging the phenotype–genotype gap via computational modeling for disease epidemiology and animal welfare

“…By integrating diverse types of omics data, these approaches can identify key components and pathways involved in immune system function and drug response [ 22 , 109 ]. This can aid in identifying novel drug targets and repurposing existing drugs for use in livestock [ 110 , 111 ]. Furthermore, network pharmacology can predict drug interactions and side effects, enabling the selection of safe and effective drug combinations for complex diseases [ 93 , 112 ].…”

The recognition of LpxC inhibitors as potential antibiotics could revolutionise the management of sepsis in veterinary patients if their unknown biological properties are widely evaluated in suitable animal models

“…Salmonella has been identified as a major cause of economic losses in domestic livestock, and is also a serious zoonotic pathogen worldwide [20]. LpxC-2 could potentially be used in controlling a wide spectrum of economically important livestock diseases such as those causing mastitis [21][22][23], endometritis, abscesses and general malaise by pathogens such as Pasteurella multocida [24], and infections caused by several members of the Enterobacteriaceae family such as Escherichia coli.…”

Atividade antimicrobiana in vitro do extrato etanólico bruto da folha da Hymenaea martiana Hayne frente às Staphylococcus spp. e avaliação de seu potencial como desinfetante em cabras

“…Para o tratamento e prevenção desta doença, foram utilizados no decorrer dos anos produtos como antibióticos e desinfetantes, ocorrendo então a seleção de micro-organismos resistentes, o que dificulta a gestão do tratamento e da prevenção da mastite em caprinos (Bhasme et al 2013). Um dos fatores a se observar, quando se trata de resistência a antibióticos, é a presença de genes de resistência nos micro-organismos, que por ventura podem ser expressos e impedir a ação dos produtos, e dentre eles está o gene blaZ (Palaniappan & Holley 2010, Wendlandt et al 2013.…”