2018
DOI: 10.1021/acs.cgd.7b01678 View full text |Buy / Rent full text
|
|

Abstract: Here we retrospectively report the successful application of the "host lipid screening" strategy in the in meso crystallization of PlsY, a bacterial glycerol 3-phosphate (G3P) acyltransferase that catalyzes the committed step in phospholipid biosynthesis. Two monoacylglycerols (MAGs) with different chain lengths, namely, the 9.9 MAG (monoolein, nine carbons on either side of the cis-double bond in the acyl chain) and the 7.8 MAG, were the two main host lipids used in this study. The native PlsY crystals growin… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
1
1
0
2
0

Year Published

2022
2022
2022
2022

Publication Types

Select...
1

Relationship

1
0

Authors

Journals

0
2
0
Order By: Relevance
“…The two examples here showed that Se-urea was not invasive under the present conditions. They both survived long soaking at an estimated concentration of 0.1 M, and both diffracted to similar quality as their native counterparts [26,41,54]. The generality of this will have to be tested with other membrane protein crystals in the future.…”
Section: Experimental Phasing Of Plsymentioning
See 1 more Smart Citation
Create an account to read the remaining citation statements from this report. You will also get access to:
  • Search over 1.2b+ citation statments to see what is being said about any topic in the research literature
  • Advanced Search to find publications that support or contrast your research
  • Citation reports and visualizations to easily see what publications are saying about each other
  • Browser extension to see Smart Citations wherever you read research
  • Dashboards to evaluate and keep track of groups of publications
  • Alerts to stay on top of citations as they happen
  • Automated reference checks to make sure you are citing reliable research in your manuscripts
  • 7 day free preview of our premium features.

Trusted by researchers and organizations around the world

Over 130,000 students researchers, and industry experts at use scite

See what students are saying

rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…The two examples here showed that Se-urea was not invasive under the present conditions. They both survived long soaking at an estimated concentration of 0.1 M, and both diffracted to similar quality as their native counterparts [26,41,54]. The generality of this will have to be tested with other membrane protein crystals in the future.…”
Section: Experimental Phasing Of Plsymentioning
“…PlsY solution was homogenized with 7.9 MAG [54] at a volume ratio of 2:3 using two coupled microsyringes [50]. Crystallization trials were carried out using a robot (Gryphon LCP, Art Robbins Instruments, Sunnyvale, CA, USA) as described [55].…”
Section: Crystallization Of Plsy In Lipid Cubic Phasementioning