Imunomodulator Properties of White Turmeric (Curcuma Mangga Val.) in Vivo

Abstract

White turmeric contains bioactive compounds that have potential as immunomodulators. This research aims to assess the antioxidant properties and quantify the total phenolic content of white turmeric in vitro and test immunomodulatory properties in vivo. The study utilized a Completely Randomized Block Design (CRBD) with 2 treatment factors, namely the variation in parts of white turmeric rhizomes (main and tiller) and the variation in blanching time using the steam blanching method (0; 2.5; 5; 7.5; and 10 min). The analyses carried out were antioxidant activity of DPPH method (2,2 diphenyl-1-pickrylhidrazyl), and total phenolic content of white turmeric. Selected white turmeric powder based on in vitro test was analysed for immunomodulator (SOD (Superoxide Dismutase), IL-1 (Interleukin-1), IL-6 (Interleukin-6), IL-8 (Interleukin-8), IgE (Immunoglobulin E), IgG (Immunoglobulin G), dan IgM (Immunoglobulin M)). Selected white turmeric is the main rhizome with 5 min steam blanching. The analysis showed antioxidant activity of 81.46% RSA and total phenolic content of 6.08 mg EAG/g db. The results of in vivo studies showed that rats given white turmeric had an SOD value of 72.92% significantly better than rats given Na-CMC (Sodium Carboxymethyl Cellulose) and not significantly different from commercial supplements (stimuno). The IL-1 value of 0.40 pg/ml and IL-8 value of 48.53 pg/ml of rats given white kunir were significantly better than rats given Na-CMC and stimuno. The antibody values of IgE 74.02 ng/ml, IgG 18.20 ng/ml and IgM 2.97 ng/ml of rats treated with white turmeric were significantly better than those treated with Na-CMC and stimuno. The antibody values indicates that white turmeric has immunomodulatory effects.