Imunodiagnóstico da cisticercose em suíno experimentalmente infectado com ovos de Taenia solium, utilizando antígeno de escólex de Cysticercus cellulosae

Soares, Killarney Ataide; Silva, M.R.M.; Poleti, Mirele Daiana; Maia, Antônio Augusto Mendes

doi:10.1590/s0102-09352006000100003

Cited by 6 publications

(6 citation statements)

References 14 publications

(4 reference statements)

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“…While the technique is very speciϐic (100%), it is not very sensitive when used on pigs with a low cyst burden (Sciutto et al 1998, Boa et al 2002, Phiri et al 2006, Soares et al 2006). …”

Section: Detection Of Circulating Antigen Using Ag-elisamentioning

confidence: 99%

“…Serodiagnosis of cysticercosis through the detection of antibodies against the parasite has been widely evaluated using target antigens ranging from total T. solium extracts of the metacestode to more selected preparations, such as cyst ϐluid, scolex or extracts from external membranes (Nunes et al 2000, Nguekam et al 2003, Soares et al 2006. Other detection methods include highly puriϐied or fractionated parasite glycoproteins (Tsang et al 1991, Aluja et al 1999 or recombinant and synthetic antigens (Sato et al 2003, Handali et al 2004, which increase the sensitivity and the speciϐicity of the tests.…”

Section: Introductionmentioning

confidence: 99%

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Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection.

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“…While the technique is very speciϐic (100%), it is not very sensitive when used on pigs with a low cyst burden (Sciutto et al 1998, Boa et al 2002, Phiri et al 2006, Soares et al 2006). …”

Section: Detection Of Circulating Antigen Using Ag-elisamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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“…A significant increase of IgG antibody titre against Cysticercus cellulosae SA in sera of pigs experimentally infected with T. solium eggs were observed after 21 days post infection till 140 days; whereas, no correlations were noted between the number of cysticerci in muscles and ELISA O.D. values ( 28 ).…”

Section: Discussionmentioning

confidence: 91%

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Satyaprakash¹,

Khan²,

Chaudhari³

et al. 2023

IJPA

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Background: Porcine cysticercosis, caused by metacestodes of Taenia solium is an important neglected zoonosis. We evaluated the presence of anti-cysticercal antibodies and T. solium specific DNA in pig sera and blood samples respectively collected from Maharashtra, India. Methods: A total of three antigens (Scolex Antigen (SA), Membrane Body Antigen (MBA) and Excretory-Secretory Antigen (ESA)) were prepared from metacestodes of T. solium and employed in an in-house developed indirect-IgG ELISA for serological screening of 1000 porcine sera samples at Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra, India. The ELISA positive sera samples were subjected to EITB Assay for detection of immunodominant peptides. An effort has been made for molecular detection of porcine cysticercosis by PCR assay targeting large subunit rRNA gene of T. solium from blood samples of the corresponding ELISA-positive pigs. Results: The overall seroprevalence of porcine cysticercosis employing SA, MBA and ESA was 12.6%, 8.7% and 12.5% respectively. The lower and medium molecular weight peptides were the most frequently recognised in EITB assay. The numbers of bands recognised in EITB assay were observed to be proportionate with the corresponding ELISA O.D. values. An amplification product of 286 bp was observed in 22.98% (20/87), 30.35% (30/99) and 17.14% (12/70) of the sero-positive samples against SA, ESA and MBA respectively. Conclusion: EITB still remains the gold standard serodiagnosis test for cysticercosis. The inclusion of a greater number of positive samples and purification of antigens may improve the diagnostic efficacy of the tests.

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“…O homem é o hospedeiro definitivo do cestódeo Taenia solium, e o suíno, hospedeiro intermediário da forma larvar, Cysticercus cellulosae. A transmissão ocorre com a ingestão de ovos de T. solium, através de alimentos ou água contaminados, que se desenvolvem na forma larvar de cisticerco com maior frequência nos tecidos conjuntivos interfascicular dos músculos sublinguais, mastigadores, diafragmático, cardíaco e no cérebro de suínos e de seres humanos (SOARES et al, 2006;FELIX et al, 2010;GARCIA et al, 2014).…”

Section: Introductionunclassified

Cisticercose suína, conhecer para previnir aspectos epidemiológicos e percepções no alto sertão sergipano

Marinho,

Faustino,

Romeiro

et al. 2023

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Editora Direitos para esta edição cedidos à Atena Editora pelos autores. Open access publication by Atena Editora Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). O conteúdo do texto e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterála de nenhuma forma ou utilizá-la para fins comerciais.Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético. Conselho Editorial Ciências Agrárias e Multidisciplinar

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Imunodiagnóstico da cisticercose em suíno experimentalmente infectado com ovos de Taenia solium, utilizando antígeno de escólex de Cysticercus cellulosae

Cited by 6 publications

References 14 publications

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Cisticercose suína, conhecer para previnir aspectos epidemiológicos e percepções no alto sertão sergipano

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