Improving the Stability and Antioxidant Properties of Sesame Oil: Water-Soluble Spray-Dried Emulsions from New Transesterified Phenolic Derivatives

Abstract

Hydrosoluble sesame oil fatty acid transesters having enhanced antioxidant activities were synthesized in a two-step process. The key step involved the biocatalyzed (lipase from Candida antarctica) acylation of sesame oil methanolic ester with either vanillyl (VNA) or piperonyl benzylic alcohols, or 5-hydroxymethyl resorcinol (5-HMR). These substrates were selected to introduce phenolic or sesamol structurally related frameworks. The VNA and 5-HMR-derived transesters were obtained with 20-40% yields and retained the starting proportions of sesame oil linoleic, oleic, and saturated acids, these fatty acids also being the only constituents of the nonesterified fraction. The VNA-derived transester showed the best antioxidant capacity in standard assays and was processed as the unique lipid phase of spray-dried emulsions containing a high level of linoleic acid phenolic ester. These emulsions provided a high degree of protection to UV-irradiated fibroblasts, through the potential synergy between VNA antioxidant action and replenishment of damaged membranes by unsaturated fatty acids.

Improving the Antioxidant Properties of Calophyllum inophyllum Seed Oil from French Polynesia: Development and Biological Applications of Resinous Ethanol-Soluble Extracts

“…Experiments were performed according to previous procedures [22,23]. Aliquots of dichloromethane solutions of oil or fractions (50-500 mg/mL) or test compounds (final concentration 0.01-2 mM) were added to 200 µL of a freshly prepared DPPH solution (75 mg/L), and the final volume was adjusted to 300 µL with dichloromethane to obtain a final DPPH concentration of 0.13 mM.…”

“…To determine the total LDH content (i.e., the sum of the enzymatic activity in the lysate and the culture medium), 1% Triton X-100 was added to randomly selected wells of untreated cells. Then, the cells were scraped and homogenized in serine-borate buffer and the MDA content of the suspension was measured by HPLC using UV-visible detection according to a previously reported procedure [22]. Results were compared to control cells treated with DMEM + 0.1% DMSO.…”

“…In our continuous interest in developing easy handling pharmaceutical preparations from natural sources of great economic potential [21,22], the purpose of the present study was (i) to prepare novel soluble TO extracts and subfractions with enhanced inhibition properties toward a host of reactive oxygen species (ROS); (ii) to screen them for their antioxidant, anti-inflammatory and antimicrobial properties using a battery of physicochemical and biochemical tests; and (iii) to further isolate and characterize in these TO fractions those compounds showing the greatest potential for biological applications.…”

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

“…Using EPR spectrometry following reported procedures (Alencar et al, 2009;Bailey et al, 2014), . HO  scavenging activity of selected extracts was determined by measuring the dosedependent inhibition of its DMPO spin adduct at room temperature.…”

A novel conversion of sesamolin to sesaminol by acidic cation exchange resin

“…Sesame ( Sesamum indicum L.) is known as one of the most important and ancient crops in the world 1. Roasted and unroasted sesame seed oils are widely used in eastern Asian countries, especially in China, Japan, and India.…”