Improving thein vitrorooting of micro-shoots of Sorbus rotundifolia ‘Bükk szépe’ by the sequential application of Humus®FW and Wuxal®Super organic and chemical fertilisers

Dobránszki, Judit; Magyar-Tábori, K.; Jevcsák, M; Ördögh, M.; Jámbor-Benczúr, E.

doi:10.1080/14620316.2012.11512903

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“…The most suitable medium for S. domestica is the Schenck and Hildebrandt [ 30 ] medium supplemented with 22.2 µM of 6-benzylaminopurine (BA) [ 26 ]; the most suitable for S. aucuparia is the MS [ 31 ] medium with 0.9–1.8 µM of BA and 0.5 µM of indole-3-butyric acid (IBA) [ 32 ]; and the most suitable for S. rotundifolia is the MS medium containing 1.6 µM of IBA, 1.4 µM of 6-benzyladenine riboside (BAR), and 0.6 µM of gibberellic acid (GA 3 ) [ 33 ]. An application of ½ MS medium supplemented with 3.3 µM BA, 0.5 µM IBA, and 0.3 µM GA 3 has also been used effectively for S. rotundifolia [ 34 ].…”

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confidence: 99%

Response of Rowan Berry (Sorbus redliana) Shoot Culture to Slow Growth Storage Conditions

Mendler-Drienyovszki

Magyar-Tábori

2023

Plants

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Slow growth storage can preserve the genetic resources of endangered species such as those of genus Sorbus. Our aim was to study the storability of rowan berry in vitro cultures, their morpho-physiological changes, and regeneration ability after different storage conditions (4 ± 0.5 °C, dark; and 22 ± 2 °C, 16/8 h light/dark). The cold storage lasted for 52 weeks, and observations were made every four weeks. Cultures showed 100% survival under cold storage, and those taken from the storage showed 100% regeneration capacity after the passages. A dormancy period lasting about 20 weeks was observed, followed by intensive shoot growth until the 48th week, which led to the exhaustion of the cultures. The changes could be traced to the reduction of the chlorophyll content and the Fv/Fm value, as well as in the discoloration of the lower leaves and the appearance of necrotic tissues. Long, etiolated shoots (89.3 mm) were obtained at the end of cold storage. Shoot cultures stored in a growth chamber as control (22 ± 2 °C, 16/8 h light/dark) senesced and died after 16 weeks. Explants from stored shoots were subcultured for four weeks. The number and length of newly developed shoots were significantly higher on explants from cold storage compared to those from control cultures if the storage was longer than one week.

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