Improving the anti‐inflammatory effect of serum eye drops using allogeneic serum permissive for regulatory T cell induction

Stenwall, Per-Anton; Bergström, M.; Seiron, Peter; Sellberg, Felix; Olsson, Tobias; Knutson, Folke; Berglund, David

doi:10.1111/aos.12801

Cited by 41 publications

(33 citation statements)

References 30 publications

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“…PA by design produces PLTs with minimal WBC content, which may explain why higher IFN‐γ levels have been reported in preparations such as two‐step centrifugation PLT‐rich plasma samples . One substantial outlier was, however, observed in this study, which is well in line with previous observations from healthy blood donors …”

Section: Discussionsupporting

confidence: 91%

Standardizing the freeze‐thaw preparation of growth factors from platelet lysate

et al. 2017

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Section: Discussionsupporting

confidence: 91%

Standardizing the freeze‐thaw preparation of growth factors from platelet lysate

et al. 2017

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“…Autologous serum eyedrops are recommended for severe dry eyes, as they contain many nutrients and proteins which can improve the extracellular matrix of the ocular surface epithelial cells by facilitating ocular surface maintenance and healing (International Dry Eye WorkShop 2007b). One study found that the anti-inflammatory cytokines were higher in serum from non-dry eye people compared to dry eye patients (Stenwall et al 2015). However, a case-control study reported that there were no significant differences in total antioxidant potential and concentration of ROS in controls and patients with dry eyes (Gus et al 2016).…”

Section: Clinical Relevancementioning

confidence: 99%

“…One study found that the anti‐inflammatory cytokines were higher in serum from non‐dry eye people compared to dry eye patients (Stenwall et al. ). However, a case–control study reported that there were no significant differences in total antioxidant potential and concentration of ROS in controls and patients with dry eyes (Gus et al.…”

Section: Clinical Relevancementioning

confidence: 99%

Dry eye disease and oxidative stress

Seen

Tong

2017

Acta Ophthalmologica

220

162

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Dry eye, an age-related condition, is a multifactorial disease of the tears and ocular surface that results in symptoms of discomfort, visual disturbance and tear film instability. Environmental factors are also often implicated in dry eye including exposure to pollutants, ultraviolet (UV) radiation and ozone as well as the chronic use of preserved eyedrops such as in the treatment of glaucoma. These factors increase oxidative stress and ocular surface inflammation. Here, we reviewed the cellular, animal and clinical studies that point to the role of oxidative stress in dry eye disease. The biomarkers used to indicate oxidative damage in ocular surface tissues include 8-hydroxy-2 deoxyguanosine (8-OHdG), 4-hydroxynonenal (HNE) and malondialdehyde (MDD). Antioxidative defences in the ocular surface occur in the form of tear proteins such as lactoferrin and S100A proteins, and enzymes such as superoxide dismutase (SOD), peroxidase, catalase and mitochondrial oxidative enzymes. An imbalance between the level of reactive oxygen species (ROS) and the action of protective enzymes will lead to oxidative damage, and possibly inflammation. A small number of interventional studies suggest that oxidative stress may be directly targeted in topical therapy of dry eye treatment. For example, in vitro studies suggest that L-carnitine and pterostilbene, a blueberry component may reduce oxidative stress, and in animal studies, alpha-lipoic acid (ALP) and selenoprotein P may be helpful. Examples of treatments used in clinical trials include vitamin B12 eyedrops and iodide iontophoresis. With recent emphasis on ageing medicine and preventive holistic health, as well as the role of environmental science, research on oxidative stress in the ocular surface is likely to have increasing impact in the coming years.

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“…It is accompanied by ocular symptoms in which unstable tear lm, hyperosmolarity, ocular surface in ammation and damage, and neurosensory abnormalities play pathologic roles [5]. Its etiologies include causes secondary to systemic autoimmune disorders such as Sjogren's syndrome, rheumatoid arthritis, systemic lupus erythematous, and so on that are bothersome to doctors and patients [6][7][8][9][10]. In conventional treatment, arti cial tear eye drops are mainly used to moisturize the ocular surface and offer additional lubrication [11].…”

Section: Introductionmentioning

confidence: 99%

Establishment of Functional Epithelial Organoid From Human Lacrimal Glands

Jeong

Choi

Lee

et al. 2020

Preprint

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Background: Tear deficiency due to dysfunction of the lacrimal gland is one of the major causes of dry eye disease (DED). Artificial tears as conventional medicine often alleviate symptoms, however, they insufficient to prevent the progression of severe DED. Lacrimal gland stem cell-based therapies have been extensively reported to regenerate injured lacrimal tissue, but the number of stem cells in the lacrimal gland is pretty low, and 2D long-term cultivation results in the reduction of the differentiation capacity of stem cells. Therefore, 3D lacrimal gland organoids could be an alternative for a DED therapeutic method, which is capable of prolonged growth while maintaining the characteristics of the lacrimal gland tissue.Method: Digested cells from human lacrimal gland tissue were mixed with Matrigel and cultured in five different media modified from human prostate/salivary organoid culture media. After organoid formation, the growth, expression of specific markers and histological characters were analyzed to prove the lacrimal gland organoids. And then, the secretory function of lacrimal gland organoids was confirmed by calcium influx or proteomics after pilocarpine treatment.Result: Human lacrimal gland organoids were formed and cultured under a modified salivary organoid culture medium, and their histological features and gene expression pattern were similar to normal lacrimal gland tissue. After pilocarpine treatment, their secretory function was confirmed and the secretomes were analyzed by proteomics. More than 70% of the identified proteins were proven to exosome through GO analysis. Additionally, to explore the curability of developed organoids, mouse-derived lacrimal gland organoids were fabricated and transplanted into mouse lacrimal tissue with DED, and we confirmed that organoids were well engrafted in the lacrimal tissue.Conclusion: These results demonstrate that our established lacrimal gland organoids resemble characteristics of normal lacrimal gland tissue and could be used as cell therapy for patients with dry eye syndrome.

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Improving the anti‐inflammatory effect of serum eye drops using allogeneic serum permissive for regulatory T cell induction

Abstract: ABSTRACT.Purpose: To investigate the cytokine composition and anti-inflammatory effects of allogeneic serum preparations for improved use as serum eye drops.

Cited by 41 publications

References 30 publications

Standardizing the freeze‐thaw preparation of growth factors from platelet lysate

Standardizing the freeze‐thaw preparation of growth factors from platelet lysate

Dry eye disease and oxidative stress

Establishment of Functional Epithelial Organoid From Human Lacrimal Glands

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