Improving ecological fitness of<i>Beauveria bassiana</i>conidia to control the sugar cane borer<i>Diatraea saccharalis</i>

Mejía, Cindy; Espinel, Carlos; Forero, Abel M.; Ramos, Freddy A.; Brandão, Pedro; Villamizar, Laura

doi:10.1080/09583157.2020.1738343

Cited by 11 publications

(10 citation statements)

References 38 publications

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“…The fungus was grown on Potato Dextrose Agar plates (PDA, Oxoid BO1010M) at 25°C in dark conditions for 10 days allowing the production of conidia required for inoculating the fermentation. Conidia for DNA extraction was produced using semi-solid fermentation on oat-based medium according to the methodology described by Mejía et al ( 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of a new strain ofMetarhizium novozealandicumwith potential to be developed as a biopesticide

et al. 2021

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The fungal species Metarhizium novozealandicum, that occurs only in New Zealand and Australia has been poorly studied. In this work, a new strain of M. novozealandicum isolated from a larva of Wiseana sp. is described based on morphology, genomic multilocus (ITS, EF-1α and β-tubulin) phylogeny, growth in different culture media and insecticidal activity. The isolate AgR-F177 was clustered in the same clade with M. novozealandicum. AgR-F177 colonies developed faster on Sabouraud Dextrose Agar (SDA) than on Potato Dextrose Agar (PDA) when incubated at 25°C, with no growth observed at 30°C on either media. Conidia yield on an oat-based medium in semisolid fermentation was 7.41 x 10 8 conidia/g of substrate and a higher yield of 1.68 x 10 9 conidia/g of substrate was obtained using solid fermentation on cooked rice. AgR-F177 formed microsclerotia (MS) in liquid fermentation after 7 days reaching the maximum yield of 3.3 × 10 3 MS/mL after 10 days. AgR-F177 caused mortality in Wiseana copularis, Costelytra giveni and Plutella xylostella larvae with efficacies up to 100%, 69.2%, and 45.7%, respectively. The ease of production of AgR-F177 with different fermentation systems and its pathogenicity against different insect pests reveal its potential as a new biopesticide.

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Section: Methodsmentioning

confidence: 99%

“…Conidia production in semisolid fermentation (SSF) was carried out following the methodology described by Mejía et al ( 2020 ) with modifications. Oats were mixed with distilled water (10% w/w) and homogenised for 1 min with a kitchen blender.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a new strain ofMetarhizium novozealandicumwith potential to be developed as a biopesticide

et al. 2021

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“…Estos resultados sugieren que los procesos de fermentación y formulación utilizados para la producción del CE no afectaron la actividad insecticida del hongo. La actividad insecticida de los microrganismos entomopatógenos es afectada por las condiciones utilizadas para su producción, como por ejemplo la composición del medio de cultivo, las fuentes de carbono y nitrógeno, el pH, la actividad de agua y la temperatura entre otros (Díaz et al, 2019;Mejía et al, 2020). En un trabajo previo, la composición del medio de cultivo y de la relación C:N del mismo, demostraron ser factores determinantes para el rendimiento ecofisiológico del aislamiento Bv062, cuyos conidios producidos en un sustrato semisólido a base de papa fueron más virulentos y estables en almacenamiento que aquellos producidos en el medio convencional a base de arroz (Mejía et al, 2020).…”

Section: Discussionunclassified

ACTIVIDAD INSECTICIDA Y PERSISTENCIA DE UN FORMULADO A BASE DE Beauveria bassiana PARA EL CONTROL DE Diatraea saccharalis

Riaño¹,

Valderrama²,

Echeverry³

et al. 2021

Chil. j. agric. anim. sci.

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El desarrollo y uso de plaguicidas microbianos para el control de plagas agrícolas se ha intensificado en los últimos años, debido a la creciente necesidad de reducir el uso de agroquímicos de alta toxicidad. Pero para suplir esta creciente necesidad de bioplaguicidas, es necesario desarrollar formulaciones adecuadas que estabilicen a los microorganismos frente a los factores ambientales de estrés que reducen su eficacia y persistencia de en campo. En este contexto, el objetivo del presente trabajo fue determinar la actividad insecticida y persistencia en plantas de caña de un concentrado emulsionable (CE) a base de Beauveria bassiana para el control del barrenador de la caña, Diatraea sp. La fotoestabilidad frente a la radiación UV tipo B y las concentraciones letales media y noventa del bioplaguicida también se determinaron bajo condiciones de laboratorio. El CE presentó una eficacia superior al 80% para el control de larvas de D. saccharalis bajo condiciones semicontroladas de campo. La formulación fotoestabilizó eficientemente los conidios, manteniendo un 68,9% de germinación después de nueve minutos de irradiación, mientras que los conidios sin formular perdieron completamente la capacidad de germinar. Este efecto también se observó para la actividad insecticida, ya que los conidios sin formular perdieron un 30% más de eficacia que los conidios formulados, después de nueve minutos de irradiación. La actividad insecticida y el recuento de conidios en plantas de caña de azúcar en campo disminuyeron a partir de la primera y segunda semana después de la aplicación, respectivamente. Aunque el CE a base de B. bassiana demostró una efectiva actividad insecticida contra D. saccharalis, es necesario realizar estudios de campo para optimizar la formulación y determinar el método y frecuencia de aplicación que permitan maximizar su desempeño.

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“…Conidia of B. bassiana Bv062 were produced and harvested according to the methodology described by Mejía et al [35]. The culture medium based on oat flour (10%) was modified by adding wheat bran at 0.1% (w/w) with water activity a w of 0.99.…”

Section: B Bassiana Bv062 Productionmentioning

confidence: 99%

“…Bv062 conidia suspended in 0.1% Tween 80 and adjusted to 2 × 10 6 con/ml were mixed in proportion 1:1 with CE or PPC (prepared in phosphate buffer pH 7), at an enzymatic activity of 0.06 and 0.12 U/ml, to obtain a final concentration of 1 × 10 6 con/ml and activity of 0.03 and 0.06 U/ml, respectively. Larva dorsa of D. saccharalis second instar larva were inoculated by applying 2 µl of the treatment with a micropipette [35]. The control treatment consisted of larvae inoculated with 0.1% Tween® 80 solution only.…”

Section: Bioassaymentioning

confidence: 99%

Use of Trichoderma koningiopsis chitinase to enhance the insecticidal activity of Beauveria bassiana against Diatraea saccharalis

et al. 2021

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Trichoderma is a well-known soil-borne fungus, highly efficient producer of extracellular enzymes including chitinases. The aim of this study was to recover a chitinase from fermentation waste after harvesting Trichoderma koningiopsis Th003 conidia and assess its potential as an enhancer of Beauveria bassiana insecticidal activity against Diatraea saccharalis. T. koningiopsis was produced by solid fermentation, conidia were harvested, and a crude extract (CE) was recovered by washing the residual substrate (rice:wheat bran). The partially purified chitinase (PPC) (75 kDa product) with N-acetyl-βglucosaminidase activity was obtained by chromatography to 29.3-fold with optimal activity at pH 5 and 55°C. Both the CE and the PPC were mixed with B. bassiana Bv062 conidia and assessed in a bioassay against D. saccharalis larvae. The CE and PPC from T. koningiopsis Th003 did not affect the germination or viability of B. bassiana conidia and enhanced its insecticidal activity when used at 0.06 U/ml enzymatic activity with a 24.5% reduction in B. bassiana lethal time (LT 90 ). This study demonstrated the potential of chitinases produced by T. koningiopsis in solid fermentation to be recovered from the waste substrate and used as an additive to enhance B. bassiana, adding value to the main waste from the Trichoderma biopesticide/biofertilizer industries.

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Improving ecological fitness ofBeauveria bassianaconidia to control the sugar cane borerDiatraea saccharalis

Cited by 11 publications

References 38 publications

Characterization of a new strain ofMetarhizium novozealandicumwith potential to be developed as a biopesticide

Characterization of a new strain ofMetarhizium novozealandicumwith potential to be developed as a biopesticide

ACTIVIDAD INSECTICIDA Y PERSISTENCIA DE UN FORMULADO A BASE DE Beauveria bassiana PARA EL CONTROL DE Diatraea saccharalis

Use of Trichoderma koningiopsis chitinase to enhance the insecticidal activity of Beauveria bassiana against Diatraea saccharalis

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