Improving cord blood typing with next-generation sequencing: impact of allele-level HLA and NIMA determination on their selection for transplantation

Enrich, Emma; Vidal, Francisco; Corrales, Irene; Campos, Eva; Borràs, Nina; Martorell, L.; Sánchez, Mar; Querol, Sergi; Rudilla, Francesc

doi:10.1038/s41409-020-0890-9

Cited by 3 publications

(2 citation statements)

References 39 publications

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“…Up-front HLA typing minimizes selection time for the best units. 63 Incorporate ultra-high-resolution NGS that extends sequencing to HLA-B leader sequences, introns and untranslated regions, 41 and non-HLA polymorphisms, for example, KIR typing, CTLA 4 64,65 etc. to refine selection choice by transplant teams.…”

Section: Quality Control Assaysmentioning

confidence: 99%

The wider perspective: cord blood banks and their future prospects

2021

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Summary Over the past three decades, cord blood transplantation (CBT) has established its role as an alternative allograft stem cell source. But the future of stored CB units should be to extend their use in updated transplant approaches and develop new CB applications. Thus, CBT will require a coordinated, multicentric, review of transplantation methods and an upgrade and realignment of banking resources and operations. Significant improvements have already been proposed to support the clinical perspective including definition of the cellular threshold for engraftment, development of transplantation methods for adult patients, engraftment acceleration with single cell expansion and homing technologies, personalised protocols to improve efficacy, use of adoptive cell therapy to mitigate delayed immune reconstitution, and further enhancement of the graft‐versus‐leukaemia effect using advanced therapies. The role of CB banks in improving transplantation results are also critical by optimizing the collection, processing, storage and characterization of CB units, and improving reproducibility, efficiency and cost of banking. But future developments beyond transplantation are needed. This implies the extension from transplantation banks to banks that support cell therapy, regenerative medicine and specialized transfusion medicine. This new “CB banking 2.0” concept will require promotion of international scientific and technical collaborations between bank specialists, clinical investigators and transplant physicians.

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Section: Quality Control Assaysmentioning

confidence: 99%

The wider perspective: cord blood banks and their future prospects

2021

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“…It has been reported that HLA compatibility in allele-level is associated with better transplant outcomes in umbilical cord blood transplantation. 1 Here, we describe the novel HLA-DRB1*12:92 and HLA-DRB1*12:101 alleles were identified by next-generation sequencing (NGS), that have been found in the donors from the Cord Blood Bank of Zhejiang Province, China, respectively.…”

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confidence: 99%