Improvement of stability and reusability of α-amylase immobilized on naringin functionalized magnetic nanoparticles: A robust nanobiocatalyst

Defaei, Mahshid; Taheri‐Kafrani, Asghar; Miroliaei, Mehran; Yaghmaei, Parichehreh

doi:10.1016/j.ijbiomac.2018.02.147

Cited by 100 publications

(41 citation statements)

References 38 publications

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“…For instance, enzymes are important biocatalysts in modern biotechnology but are highly unstable in nature. The thermal, pH, and storage stability of α-amylase could be improved by immobilization with naringin-functionalized magnetite nanoparticles [ 210 ]. Ag NPs synthesized using O. sanctum and Chenopodium aristatum showed good catalytic activity in degradation of 4-nitrophenol [ 21 , 22 ].…”

Section: Potential Applications Of Green-synthesized Npsmentioning

confidence: 99%

Secondary Metabolites in the Green Synthesis of Metallic Nanoparticles

Marslin¹,

et al. 2018

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The ability of organisms and organic compounds to reduce metal ions and stabilize them into nanoparticles (NPs) forms the basis of green synthesis. To date, synthesis of NPs from various metal ions using a diverse array of plant extracts has been reported. However, a clear understanding of the mechanism of green synthesis of NPs is lacking. Although most studies have neglected to analyze the green-synthesized NPs (GNPs) for the presence of compounds derived from the extract, several studies have demonstrated the conjugation of sugars, secondary metabolites, and proteins in these biogenic NPs. Despite several reports on the bioactivities (antimicrobial, antioxidant, cytotoxic, catalytic, etc.) of GNPs, only a handful of studies have compared these activities with their chemically synthesized counterparts. These comparisons have demonstrated that GNPs possess better bioactivities than NPs synthesized by other methods, which might be attributed to the presence of plant-derived compounds in these NPs. The ability of NPs to bind with organic compounds to form a stable complex has huge potential in the harvesting of precious molecules and for drug discovery, if harnessed meticulously. A thorough understanding of the mechanisms of green synthesis and high-throughput screening of stabilizing/capping agents on the physico-chemical properties of GNPs is warranted to realize the full potential of green nanotechnology.

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Section: Potential Applications Of Green-synthesized Npsmentioning

confidence: 99%

Secondary Metabolites in the Green Synthesis of Metallic Nanoparticles

Marslin¹,

et al. 2018

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“…Free PLC was connected with Fe 3 O 4 @ SiO 2 @p(GMA) in covalent bond and changed the secondary structure of free PLC, increasing the rigidity of PLC structure, changing the conformation of immobilized enzyme that made it more suitable for contact with substrate, reducing the denaturant at high temperature, and improving its thermal stability [27,28]. Defaei et al immobilized α-amylase onto naringin functionalized MNPs that exhibited a good thermostability [29]. Candida antarctica lipase B was immobilized on Fe 3 O 4 @SiO 2 @p(GMA); its optimum temperature was increased by 5°C and had higher activities at a wider range of temperatures [30].…”

Section: Enzymatic Characteristics Of Nanomagnetic Immobilized Plc: Ementioning

confidence: 99%

Synthesis and Application of Nanomagnetic Immobilized Phospholipase C

Jiang

Tang

et al. 2019

Journal of Chemistry

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The nanomagnetic carrier (Fe3O4@SiO2@p(GMA)) was prepared by atom transfer radical polymerization, and then, the free phospholipase C (PLC) was immobilized on it proved by the results of FT-IR analysis. The enzyme loading was 135.64 mg/g, the enzyme activity was 8560.7 U/g, the average particle size was 99.86 ± 0.80 nm, and the specific saturation magnetization was 16.00 ± 0.20 emu/g. PLC-Fe3O4@SiO2@p(GMA) showed the highest activities at the pH of 7.5, and tolerance temperature raised to 65°C in soybean lecithin emulsion. Enzymatic degumming with PLC-Fe3O4@SiO2@p(GMA) under the conditions of the enzyme dosage of 110 mg/kg, reaction temperature of 65°C, pH of 7.5, and reaction time of 2.5 h resulted in residual phosphorus of 64.7 mg/kg, 1,2-diacylglycerol (1,2-DAG) contents of 1.07%, and oil yield of 98.1%. Moreover, PLC-Fe3O4@SiO2@p(GMA) still possessed more than 80% of its initial activity after 5 cycles.

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“…Surface modification processes, including immobilization of enzyme, were evaluated by Defaei et al [33]. The authors synthesized magnetic nanoparticles coated with silica and functionalized with naringin (MNP@SiO 2 /NA).…”

Section: Magnetization Measurementsmentioning

confidence: 99%

“…Defaei et al [33] demonstrated by SEM and TEM techniques the immobilization of α-amylase on magnetic nanoparticles. A slight increase in the particle size after the immobilization process suggested the effective enzyme immobilization.…”

Section: Scanning Electronic Microscopy (Sem) and Transmission Electrmentioning

confidence: 99%

“…TGA can also be employed to determine the degree of functionalization of the support, the effectiveness of the immobilization method, and structural information of biomolecule after the immobilization [40]. An attractive nanobiocatalyst consisting of α-amylase (AA) immobilized on magnetic nanoparticles (MNP@SiO 2 ) functionalized with naringin (NA) was proposed [33]. The functionalization and immobilization processes were assessed by TGA analysis.…”

Section: Thermal Gravimetric Analysis (Tga)mentioning

confidence: 99%

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Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Cabrera¹,

Neri²,

Soria³

et al. 2019

Applied Surface Science

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In this chapter, the authors will show different proposals of magnetic bio-derivatives and its applicability in biotechnology. The historical context of immobilized enzymes, as well as highlighting the main advantages and disadvantages of each, will be mentioned. Besides, iron oxides and composite materials will be presented as support for biomolecules immobilization. Composites are effortlessly prepared including many materials capable of providing advantages to the magnetic derivatives. Enzymes covalently linked to these magnetic particles combine their catalytic properties with reaction specificity, reusability, and possible reactor construction. In addition, proteins can also be purified by these magnetic composites containing specific ligands allowing reactors and reuses too. Some characterization techniques used to study the magnetic material and derivative immobilized will be described as well. Altogether, an engaging presentation about the interesting features of magnetic bio-derivatives will highlight their uses in biotechnology field as well as in others.

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Improvement of stability and reusability of α-amylase immobilized on naringin functionalized magnetic nanoparticles: A robust nanobiocatalyst

Cited by 100 publications

References 38 publications

Secondary Metabolites in the Green Synthesis of Metallic Nanoparticles

Secondary Metabolites in the Green Synthesis of Metallic Nanoparticles

Synthesis and Application of Nanomagnetic Immobilized Phospholipase C

Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

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