Improvement of Modified Charcoal-Cefoperazone-Deoxycholate Agar by Supplementation with a High Concentration of Polymyxin B for Detection of Campylobacter jejuni and C. coli in Chicken Carcass Rinses

Abstract

Modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by supplementation with a high concentration of polymyxin B. The ability of the supplemented medium to isolate Campylobacter jejuni and C. coli from chicken carcass rinses was compared to that of Campy-Cefex agar and mCCDA. Modification of mCCDA with increased polymyxin B yielded a significantly (P < 0.05) higher isolation rate and greater selectivity than those achieved using Campy-Cefex agar and mCCDA.

Restoring the selectivity of modified charcoal cefoperazone deoxycholate agar for the isolation of Campylobacter species using tazobactam, a β-lactamase inhibitor

“…Chon et al (2012) reported that the addition of polymyxin B restored some of the mCCDA medium's selectivity for Campylobacter and inhibited E. coli; however resistance could develop over time (Urban et al, 2011). In contrast our approach of adding the tazobactam β-lactamase inhibitor circumvents this potential problem as no additional antimicrobial is used; rather the compound is simply restoring the efficacy of cefoperazone against ESBL-producing organisms by competitive inhibition.…”

“…coli grows more rapidly (24h) under the same incubation conditions used for the isolation of Campylobacter (37-42°C; 5-10% O 2 ; 10% CO 2 , 48h) (Chon et al, 2013). Previous researchers have tried various methods to remove ESBL E. coli contamination on various Campylobacter selective media (Chon et al, 2012;Moran et al, 2011;Jasson et al, 2009). Some success has been achieved at inhibiting ESBL E. coli and restoring selectivity for Campylobacter with β-lactamase inhibitors.…”

“…The use of ESBL inhibitors as a mean to modify mCCDA media may be more advantageous than recent attempts where extra antimicrobials such as polymyxin B were added (Chon et al, 2012). Chon et al (2012) reported that the addition of polymyxin B restored some of the mCCDA medium's selectivity for Campylobacter and inhibited E. coli; however resistance could develop over time (Urban et al, 2011).…”

“…In recent years the isolation of Campylobacter from various types of poultry samples has become problematic due to the emergence of antimicrobial resistant contaminants, in particular β lactam resistant E. coli, which has been reported as a contaminant previously using both mCCDA (Chon et al, 2012) and Bolton broth (Jasson et al, 2009;Moran et al, 2011). ESBL E. coli are particularly problematic on Campylobacter selective media given that they can grow under the same microaerophilic conditions as Campylobacter and share the same optimum temperatures 37-42°C.…”

Improvement of Karmali Agar by Addition of Polymyxin B for the Detection of Campylobacter jejuni and C. coli in Whole‐Chicken Carcass Rinse

“…A total of 80 whole chickens were purchased from October 2011 to February 2012 from grocery stores in Seoul, South Korea. All experimental procedures for detecting C. jejuni and C. coli were performed in accordance with our previous study with minor modification (Chon and others ). The chicken carcasses were rinsed with 400 mL of buffered peptone water (Difco, Sparks, M.D., U.S.A.).…”