Abstract:Biotechnological production of glycosides is an economically competitive manufacturing alternative to classical chemical synthesis. Due to continuous improvement in production, glycosides can now be used in low-cost products by various industries. However, many production systems still suffer from low yields.Directed evolution, coupled with a suitable screening method, can tackle this challenge. We generated glycosyltransferase mutants through error-prone-PCR and screened the library using a small-scale whole-… Show more
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