Improved sphincter contractility after allogenic muscle-derived progenitor cell injection into the denervated rat urethra

Cannon, Tracy W.; Lee, Ji Youl; Somogyi, George T.; Pruchnic, Ryan; Smith, Christopher P.; Huard, Johnny; Chancellor, Michael B.

doi:10.1016/s0090-4295(03)00679-4

Cited by 151 publications

(86 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among these, MDSCs are especially promising because the cells are muscle progenitor cells that have shown the capacity to differentiate into bone, cartilage, nerve, and endothelium (20,21). MDSCs have the potential to improve sphincter function by remodeling the damaged urethra, as well as acting to generate tissues with bulking properties (23,24). However, MDSC injection therapy has certain disadvantages including migration and absorption, as is the case for other commercial injection agents.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model

Song

Lin

2010

Braz J Med Biol Res

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model

Song

Lin

2010

Braz J Med Biol Res

View full text Add to dashboard Cite

“…This is of particular interest when patients' own stem cells are used as a therapeutic means to restore the collagen homeostasis. Stem cells have been used to create striated sphincter muscle in vitro [93]. Also, autologous myoblasts and fibroblasts were injected lateral from the urethra to treat urinary incontinence [94][95].…”

Section: Summery and Perspectivementioning

confidence: 99%

Changes in connective tissue in patients with pelvic organ prolapse—a review of the current literature

2008

View full text Add to dashboard Cite

Little is known about the pathophysiology of pelvic organ prolapse (POP). In 1996, Jackson presented a hypothesis on pelvic floor connective tissue that tried to explain the development of POP on a molecular level. The objective of this review is to test the hypothesis against recent literature. The method used was a review of literature. The association between POP and connective tissue metabolism is well established. However, the causality of this association is unclear. The characteristics of the pelvic floor connective tissue of POP patients relate to tissue repair. To resolve the question of cause and effect, the role of fibroblasts in producing the extracellular matrix should be clarified. With these data, the use of autologous or allogenic stem cells in the treatment of POP may come in sight. Recent literature supports the hypothesis of Jackson but does not resolve long-standing questions on the aetiology of POP.

show abstract

“…Mesenchymal stem cells utilized in several studies were obtained from a variety of adult and fetal tissues [5][6][7][8]. So far, these adult mesenchymal stem cells have yielded promising results in preclinical and animal studies [9][10][11][12][13][14][15]. The SC-like cells for transplantation obtained from these adult tissues contain a heterogeneous mixture of mesenchymal cells, such as myoblasts at various stages of differentiation originating from satellite cells, mesenchymal stem cells, striated muscle cells, and fibroblasts.…”

mentioning

confidence: 99%

Smooth Muscle Precursor Cells Derived from Human Pluripotent Stem Cells for Treatment of Stress Urinary Incontinence

Wang

Wen

et al. 2016

Stem Cells and Development

View full text Add to dashboard Cite

There is great interest in using stem cells (SC) to regenerate a deficient urethral sphincter in patients with urinary incontinence. The smooth muscle component of the sphincter is a significant contributor to sphincter function. However, current translational efforts for sphincter muscle restoration focus only on skeletal muscle regeneration because they rely on adult mesenchymal SC as cell source. These adult SC do not yield sufficient smooth muscle cells (SMCs) for transplantation. We may be able to overcome this limitation by using pluripotent stem cell (PSC) to derive SMCs. Hence, we sought to investigate whether smooth muscle precursor cells (pSMCs) derived from human PSCs can restore urethral function in an animal model generated by surgical urethrolysis and ovariectomy. Rats were divided into four groups: control (no intervention), sham saline (surgery + saline injection), bladder SMC (surgery + human bladder SMC injection), and treatment (surgery + pSMC injection, which includes human embryonic stem cell (hESC) H9-derived pSMC, episomal reprogrammed induced pluripotent stem cells (iPSCs)-derived pSMC, or viral reprogrammed iPSC-derived pSMC). pSMCs (2 · 10 6 cells/rat) were injected periurethrally 3 weeks postsurgery. Leak point pressure (LPP) and baseline external urethral sphincter electromyography were measured 5 weeks postinjection. Both iPSC-derived pSMC treatment groups showed significantly higher LPP compared to the sham saline group, consistent with restoration of urethral sphincter function. While the difference between the H9-derived pSMC treatment and sham saline group was not significant, it did show a trend toward restoration of the LPP to the level of intact controls. Our data indicate that pSMCs derived from human PSCs (hESC and iPSC) can restore sphincter function.

show abstract

Improved sphincter contractility after allogenic muscle-derived progenitor cell injection into the denervated rat urethra

Cited by 151 publications

References 16 publications

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model

Changes in connective tissue in patients with pelvic organ prolapse—a review of the current literature

Smooth Muscle Precursor Cells Derived from Human Pluripotent Stem Cells for Treatment of Stress Urinary Incontinence

Contact Info

Product

Resources

About