Improved single-cell gel electrophoresis assay for detecting DNA damage inEisenia foetida

Marzio, Walter Darío Di; Sáenz, María Elena; Lemière, Sébastien; Vasseur, Paule

doi:10.1002/em.20153

Cited by 43 publications

(26 citation statements)

References 14 publications

(15 reference statements)

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“…Di Marzio et al [2005] observed no genotoxic effect on earthworms after 2 and 7 days of exposure but after 15 and 21 days of exposure to a PAH-contaminated soil with concentrations 5 or 10 times below ours (S 16 PAHs 5 96 mg/kg d.w.). Qiao et al [2007] demonstrated genotoxicity in earthworms after 1, 3, 7, and 14 days of exposure to soils irrigated by wastewaters contaminated with PAHs and other persistent organic compounds.…”

Section: Discussionmentioning

confidence: 85%

“…Yet, studies dealing with the genotoxicity of field-contaminated soils to earthworms are rare [White and Claxton, 2004]. Few authors have studied the genotoxicity to earthworms of PAH-contaminated soils [Šalagovič et al, 1996;Di Marzio et al, 2005] or multicontaminated field soils [Verschaeve and Gilles, 1995;Xiao et al, 2006b;Qiao et al, 2007].…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Bonnard¹,

Eom²,

Morel³

et al. 2008

Environ and Mol Mutagen

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Polluted soil sampled from a former coking plant in Lorraine (France) was studied for its genotoxicity and reproductive effects on the Eisenia fetida earthworm. Genotoxicity was investigated by means of the single-cell gel electrophoresis (comet) assay on the coelomocytes of earthworms after 4 and 10 days of exposure to the soil. DNA damage and a decline in the number of coelomocytes extruded from earthworms were observed at coking plant soil concentrations of 20 and 40% (w/w) in ISO soil. These soil concentrations had previously been shown to significantly reduce cocoon and juvenile productions after 28 and 56 days of earthworm exposure, respectively. The results showed that genotoxic pollutants in the tested soil were still bioavailable despite the age of the contaminated soil. Similar values of the no-observedeffect concentration (NOEC) corresponding to 10% of the contaminated soil and of the lowest soil concentration tested inducing effects (LOEC) corresponding to 20% of the contaminated soil were obtained from reproductive and genotoxicity endpoints. Among the soil pollutants measured, polycyclic aromatic hydrocarbons (PAHs) appeared to be the most likely source of the genotoxicity recorded, although effects of metals could not be excluded. Measurement of genotoxicity in earthworms could complement the existing standardized tests used in the ecotoxicological assessment of the risk associated with contaminated soils. Environ. Mol. Mutagen. 50:60-67, 2009.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Bonnard¹,

Eom²,

Morel³

et al. 2008

Environ and Mol Mutagen

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“…Experiments were performed in triplicate and 900 cells were analyzed per each fish. Only comets with similar size and shape nuclei were scored to avoid heterogeneous response of differing cell types present in the cell suspension [22].…”

Section: Onestep Rt-pcrmentioning

confidence: 99%

“…Cell viability was determined using 0.4% of Trypan blue. A single-cell electrophoresis assay (SCEA) was carried out according to Di Marzio et al [22]. Basically, the gels were composed of three layers of agarose.…”

Section: Onestep Rt-pcrmentioning

confidence: 99%

Environmental impact of insecticides applied on biotech soybean crops in relation to the distance from aquatic ecosystems

Marzio

Sáenz

Alberdi

et al. 2010

Enviro Toxic and Chemistry

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Aquatic environments located in areas cultivated with biotech soybean were studied. Water and sediment samples were analyzed for insecticides, acute toxicity, genotoxicity, detoxification biomarkers, and fish diversity. Samples were taken in the core area of soybean cultivation in Argentina; all measures were related to the distance between the crops and the streams sampled. Endosulfan (alpha + beta) concentrations as high as 553.33 microg/kg were found in sediments from environments located at 0.15 m from treated fields. Ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P4501A1 (CYP1A1) gene expression in fish showed the highest correlation with the environmental concentration of endosulfan. These biomarkers and mortality of amphipods significantly correlated with the concentration of endosulfan in water and sediment, which correlates inversely with the distance between the crop and streams. The differences with respective controls disappear at distances greater than 5 m. The fish diversity was significantly lower from distances between the margin of the stream and soybean crops, not exceeding 2 m.

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“…This result was consistent when the process of brown body formation (by aggregation of coelomocytes) was considered (Valembois et al 1992). This relation could be supported indirectly by the findings, including the dependence of the quantitative composition of coelomocytes on individual age, which was mentioned in some studies (Adamowicz and Wojtaszek 2001;Di Marzio et al 2005). On the contrary to the findings mentioned above, Dales (1964) reported that the size of coelomocytes changed independently from the body size of an adult during the growth.…”

Section: Discussionmentioning

confidence: 72%

Size-associated Variation and Factors Affecting the Morphology of Brown Bodies in Glycera tridactyla (Polychaeta:Glyceridae)

Can

2014

Braz. arch. biol. technol.

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Size-associated variations in brown body morphology (size and shape) were studied from the individuals of Glycera tridactyla. Variables related to size, length (BL) and width (BW) of the brown bodies were measured. On the basis of these measurements, other variables such as the surface area (SA), volume (V), surface area/volume ratio (SA/V) and elongation degree (ED) were calculated. Brown body shape was quantified by the elongation degree. The immature brown bodies were significantly smaller than the mature bodies, and therefore the smaller bodies had a higher SA/V. Results obtained from the regression analysis showed that there was a significant relationship between all the variables with the exception of SA/V:BW for the immature bodies and ED: BL for both groups of the bodies.The body size (proboscis length) of the worm and the sampling time (months) were the affecting factors on size and shape of the brown body. Brown bodies tend to be elongated as the proboscis length increased. The variation in size and/or shape of the brown bodies could refer to an adaptation to the movement along the coelomic fluid.

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Improved single-cell gel electrophoresis assay for detecting DNA damage inEisenia foetida

Cited by 43 publications

References 14 publications

Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Genotoxic and reproductive effects of an industrially contaminated soil on the earthworm Eisenia Fetida

Environmental impact of insecticides applied on biotech soybean crops in relation to the distance from aquatic ecosystems

Size-associated Variation and Factors Affecting the Morphology of Brown Bodies in Glycera tridactyla (Polychaeta:Glyceridae)

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