Improved Separation of Vitamin B Complex and Folic Acid Using Some New Solvent Systems and Impregnated TLC

Abstract

TLC separation of vitamins of the 'B' complex group and folic acid has been achieved on plates impregnated with different transition metal ions. The metal ions used were Mn ++ , Fe ++ , Co ++ , Ni ++ , Cu ++ , Zn ++ and Hg ++ . hR f values for all the vitamins, using six new solvent systems, worked out for the purpose, for each of the four different concentrations of each metal ion have been reported. The results have been discussed for each metal ion compared and the best conditions of separation have been identified.

Separation of cephalosporins on thin silica gel layers impregnated with transition metal ions and by reversed‐phase TLC

“…Impregnation of silica gel layers with transition metal ions has been shown to improve separation of amino acids (Bhushan and Parshad, 1994), antihistamines (Bhushan and Joshi, 1996), vitamin B complex and folic acid (Bhushan and Parshad, 1999). Some cephalosporins such as cefadroxil, are also known to form complexes (Shoukry et al, 1997).…”

Hydrophilic Vitamins

“…HO HO [4] ' ono I £°^o HO O-P-O-P-OH OH Figure 6 Structural formulas of (1) nicotinic acid, (2) nicotinamide, and related compounds 3, nicotinamide adenine dinucleotide (NAD + ) and 4, nicotinamide adenine dinucleotide phosphate (NADP + ). acid sodium and calcium salts are often used as additives because they are easily handled and stable.…”

“…Separation of vitamin B complex (vitamin B,, B 2 , B 6 , B 12 , and folic acid) by TLC was achieved on plates impregnated with various transition metal ions (4). The metal ions used were Mn 2+ , Fe 2+ , Co 24 , Ni 2^, Cu 2+ , Zn 2+ , and Hg 2+ .…”

Separation of amino acids, their derivatives and enantiomers by impregnated TLC

“…Recently, quantitative determination of alkanes by fluorescence scanning densitometry using silica gel plates impregnated with berberine sulphate has been reported (Cossio et al, 2000). TLC studies on separation of amino acids on copper sulphate and polyamide mixed silica gel layers (Srivastava et al, 1984a) using MeOH-BuOAc-HOAcpyridine, separation of synthetic dyes on copper sulphate and ammonium molybdate impregnated silica layers , separation of certain metal ions on 8-hydroxyquinoline and dibenzoylmethane impregnated layers (Srivastava et al, 1984b), separation of rare earths on HDEHP-TOPO impregnated cellulose plates , improved separation of several PTH-amino acids on Fe 2 , Co 2 , Ni 2 and Zn 2 impregnated silica gel plates (Bhushan and Reddy, 1987a, b), and separation of antihistamines (Bhushan and Joshi, 1996) and vitamins (Bhushan and Parshad, 1999) on plates impregnated with metal ions are a few examples from the literature. Impregnated TLC can provide direct resolution of enantiomers of a variety of compounds (Martens and Bhushan, 1988Bhushan and Martens, 1996, including 2-arylpropionic acids (Bhushan and Thiong'o, 1999), b-adrenergic blockers (Bhushan and Thiong'o, 1998), and racemic flavanones (Lepri et al, 1999a).…”