Improved Preservation and Microcirculation with POLYSOL After Partial Liver Transplantation in Rats

Yagi, Shintaro; Doorschodt, Benedict M.; Afify, Mohamed; Klinge, U.; Kobayashi, Eiji; Üemoto, Shinji; Tolba, René

doi:10.1016/j.jss.2010.12.040

Cited by 36 publications

(33 citation statements)

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“…Rat P-OLT is a complex microsurgical model which is appropriate for answering a great number of scientific questions concerning the following topics: liver regeneration [5,6], small-for-size syndrome [7], rejection and tolerance [8], and graft ischemia-reperfusion injury [9], among others. Despite its feasibility, the literature is confusing regarding the technical aspects of rat P-OLT, and most of the research groups use their well-tried ‘in-house' protocols, which makes a comparison of their findings difficult.…”

Section: Introductionmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

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Section: Introductionmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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“…• C as described in detail previously (6). We studied the three experimental groups (n = 20 for each): (A) control group (n = 20), cold static storage; (B) VSOP group (n = 20), VSOP during cold storage and (C) VSOP+NO group (n = 20), VSOP supplemented with 40 ppm-NO gas (optimal NO concentration from results of Experiment I) during cold storage.…”

Section: Experimental Groups and Designmentioning

confidence: 99%

“…We evaluated hepatic microcirculation and portal circulation at the time of euthanasia before collecting blood or tissue samples as described previously (6). As a reference (baseline), we measured hepatic microcirculation and portal circulation immediately after laparotomy.…”

Section: Evaluation Of Hepatic Microcirculation and Portal Circulatiomentioning

confidence: 99%

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A Novel Organ Preservation for Small Partial Liver Transplantations in Rats: Venous Systemic Oxygen Persufflation With Nitric Oxide Gas

Yagi

Nagai

Kadaba

et al. 2013

American Journal of Transplantation

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The prognosis for recipients of small liver grafts is poor. The aim of this study was to determine the impact of venous systemic oxygen persufflation (VSOP) with nitric oxide (NO) gas for 30% partial liver preservation and transplantation in rats. After we determined optimal NO concentration as 40 ppm in vitro with the isolated perfused rat liver model, we assessed liver injury and regeneration in vivo at 1, 3, 24 and 168 h after transplantation in the following three groups after 3 h-cold storage (n = 20 per group): control group = static storage; VSOP group = oxygen persufflation and VSOP+NO group = oxygen with NO persufflation. The liver graft persufflation was achieved with medical gas via the suprahepatic vena cava; In comparison with control group after transplantation, VSOP+NO preservation (1) increased portal circulation, (2) reduced AST and ALT release, (3) upregulated hepatic endothelial NO synthase, (4) reduced hepatocyte and bileductule damage and (5) improved liver regeneration. These results suggest that gaseous oxygen with NO persufflation is a novel and safe preservation method for small partial liver grafts, not only alleviating graft injury but also improve liver regeneration after transplantation.

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AbstractOrthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as studies on graft preservation and ischemia-reperfusion injury 1,2 , immunological responses 3,4 , hemodynamics 5,6 , and small-for-size syndrome 7 . The rat OLT is among the most difficult animal models in experimental surgery and demands advanced microsurgical skills that take a long time to learn.

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confidence: 99%

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Nagai

Yagi

Üemoto

et al. 2013

JoVE

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Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as studies on graft preservation and ischemia-reperfusion injury 1,2 , immunological responses 3,4 , hemodynamics 5,6 , and small-for-size syndrome 7 . The rat OLT is among the most difficult animal models in experimental surgery and demands advanced microsurgical skills that take a long time to learn. Consequently, the use of this model has been limited. Since the reliability and reproducibility of results are key components of the experiments in which such complex animal models are used, it is essential for surgeons who are involved in rat OLT to be trained in wellstandardized and sophisticated procedures for this model.While various techniques and modifications of OLT in rats have been reported 8 since the first model was described by Lee et al. 9 in 1973, the elimination of the hepatic arterial reconstruction 10 and the introduction of the cuff anastomosis technique by Kamada et al. 11 were a major advancement in this model, because they simplified the reconstruction procedures to a great degree. In the model by Kamada et al., the hepatic rearterialization was also eliminated. Since rats could survive without hepatic arterial flow after liver transplantation, there was considerable controversy over the value of hepatic arterialization. However, the physiological superiority of the arterialized model has been increasingly acknowledged, especially in terms of preserving the bile duct system 8,12 and the liver integrity 8,13,14 .In this article, we present detailed surgical procedures for a rat model of OLT with hepatic arterial reconstruction using a 50% partial graft after ex vivo liver resection. The reconstruction procedures for each vessel and the bile duct are performed by the following methods: a 7-0 polypropylene continuous suture for the supra-and infrahepatic vena cava; a cuff technique for the portal vein; and a stent technique for the hepatic artery and the bile duct. Video LinkThe video component of this article can be found at https://www.jove.com/video/4376/ Protocol Basic Techniques and Common Procedures1. All procedures are carried out under clean but nonsterile conditions. 2. Once the abdomen of the rat is opened, all procedures are performed under a surgical microscope at a magnification of 16x. Exceptions are the ex vivo liver resection, which is performed at 10x, and the following procedures, which are performed at 25x: insertion of a stent into the bile duct and the hepatic artery, and the reconstruction of the hepatic artery, the infrahepatic vena cava (IHVC), and the bile duct. 3. Cotton swabs are used for gentle manipulation of organs, blunt dissection of tissues, and compression hemostasis. Gauze swabs (5 x 5 cm) soaked with lactated Ringer solution are used to retract the liver or intestines, and to keep organs moist. The Satinsky clamp can be used for retraction of gauze-covered intestines toward the left or the tail of the ...

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Improved Preservation and Microcirculation with POLYSOL After Partial Liver Transplantation in Rats

Cited by 36 publications

References 24 publications

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

A Novel Organ Preservation for Small Partial Liver Transplantations in Rats: Venous Systemic Oxygen Persufflation With Nitric Oxide Gas

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

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