Improved method for chick whole‐embryo culture using a filter paper carrier

Chapman, Steve; Collignon, Jérôme; Schoenwolf, Gary C.; Lumsden, Andrew

doi:10.1002/1097-0177(20010301)220:3<284::aid-dvdy1102>3.3.co;2-x

Cited by 134 publications

(198 citation statements)

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“…The embryo culture was prepared as described by (Chapman et al, 2001). The embryo was first turned upside down (ventrally) and the endoderm above the area of operation was removed then a drop of 0.05% pancreatin (Gibco-BRL) in calcium free PBS was applied for 1 min.…”

Section: Embryo Manipulationsmentioning

confidence: 99%

Shh regulates chick Ebf1 gene expression in somite development

El-Magd¹,

Allen²,

McGonnell³

et al. 2015

Gene

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Section: Embryo Manipulationsmentioning

confidence: 99%

Shh regulates chick Ebf1 gene expression in somite development

El-Magd¹,

Allen²,

McGonnell³

et al. 2015

Gene

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“…Chick embryos were isolated and placed in a modified New culture for in vitro manipulation (Chapman et al, 2001). Briefly, eggs were placed into a 10 cm plate, and the albumen was scraped off of the embryonic region with a razor blade.…”

Section: Chick Embryo Isolation and Culturementioning

confidence: 99%

FGF signaling is necessary for establishing gut tube domains alongthe anterior–posterior axis in vivo

Dessimoz

Opoka

Kordich

et al. 2006

Mechanisms of Development

165

130

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At the end of gastrulation in avians and mammals, the endoderm germ layer is an undetermined sheet of cells. Over the next 24-48 h, endoderm forms a primitive tube and becomes regionally specified along the anterior-posterior axis. Fgf4 is expressed in gastrulation and somite stage embryos in the vicinity of posterior endoderm that gives rise to the posterior gut. Moreover, the posterior endoderm adjacent to Fgf4-expressing mesoderm expresses the FGF-target genes Sprouty1 and 2 suggesting that endoderm respond to an FGF signal in vivo. Here, we report the first evidence suggesting that FGF4-mediated signaling is required for establishing gut tube domains along the A-P axis in vivo. At the gastrula stage, exposing endoderm to recombinant FGF4 protein results in an anterior shift in the Pdx1 and CdxB expression domains. These expression domains remain sensitive to FGF4 levels throughout early somite stages. Additionally, FGF4 represses the anterior endoderm markers Hex1 and Nkx2.1 and disrupts foregut morphogenesis. FGF signaling directly patterns endoderm and not via a secondary induction from another germ layer, as shown by expression of dominant-active FGFR1 specifically in endoderm, which results in ectopic anterior expression of Pdx1. Loss-of-function studies using the FGF receptor antagonist SU5402 demonstrate that FGF signaling is necessary for establishing midgut gene expression and for maintaining gene expression boundaries between the midgut and hindgut from gastrulation through somitogenesis. Moreover, FGF signaling in the primitive streak is necessary to restrict Hex1 expression to anterior endoderm. These data show that FGF signaling is critical for patterning the gut tube by promoting posterior and inhibiting anterior endoderm cell fate.

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“…We utilised a chick embryo culture method that allows embryos to be maintained upon a semi-solid base for up to 24 h and facilitates manipulation and treatment with exogenous reagents (Chapman et al 2001). This technique was used in conjunction with the plant steroidal alkaloid, cyclopamine (obtained from Toronto Research Chemical, Canada), a potent inhibitor of Shh signalling (Taipale et al 2000).…”

Section: Easy Cultures and Cyclopamine Treatmentsmentioning

confidence: 99%

“…Chicken IGF-I and -II were also included to establish where there was overlap of expression with receptor and ligand. Finally, we employed cyclopamine treatment of embryos in EASY cultures (Chapman et al 2001) to establish whether the action of IGFBP-5 lies downstream in a signalling pathway from the morphogen Sonic Hedgehog (Shh) during early chick embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Major components of the insulin-like growth factor axis are expressed early in chicken embryogenesis, with IGF binding protein ( IGFBP ) -5 expression subject to regulation by Sonic Hedgehog

Allan

Zannoni

McKinnell

et al. 2003

Anatomy and Embryology

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Using whole-mount in situ hybridisation techniques, we have examined the expression of major components of the insulin-like growth factor (IGF) axis in early development of the chicken embryo, including both IGF-I and -II, the type 1 IGF receptor ( IGFR), and two of the IGF binding proteins, ( IGFBP) -2 and -5. We report that these genes fall into two distinct groups with respect to expression pattern, with IGFBP-2 displaying broad overlap of mRNA expression with IGFR and IGF-I during early development, whereas the expression profile of IGFBP-5 most closely resembled that of IGF-II. Comparison between different stages revealed IGFBP-2 mRNA was detected as early as stage 3, whereas IGFBP-5 was first seen at stage 4. In addition, we detected expression domains of IGFBP-5, and to a lesser extent IGFBP-2, which did not overlap with either IGFR or IGF expression patterns. This could indicate IGF independent actions of the IGFBPs during early embryonic development. A striking observation concerning the expression profiles of both IGF-II and IGFBP-5 at early stages of chick embryogenesis is that both these genes are expressed asymmetrically in a pattern similar to that of Sonic Hedgehog (Shh). Furthermore, using cyclopamine, we have demonstrated that IGFBP-5 expression in the early embryo is regulated by Shh. Taken together, these results describe an important role for the IGF system in the very early stages of the developing chicken embryo, and imply that IGFBP-2 and -5 are fundamental developmental factors, with the latter involved in Shh signalling pathways.

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Improved method for chick whole‐embryo culture using a filter paper carrier

Cited by 134 publications

References 0 publications

Shh regulates chick Ebf1 gene expression in somite development

Shh regulates chick Ebf1 gene expression in somite development

FGF signaling is necessary for establishing gut tube domains alongthe anterior–posterior axis in vivo

Major components of the insulin-like growth factor axis are expressed early in chicken embryogenesis, with IGF binding protein ( IGFBP ) -5 expression subject to regulation by Sonic Hedgehog

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