Improved functions of human hepatocytes on NH3 plasma-grafted PEEK-WC–PU membranes

Salerno, Simona; Piscioneri, Antonella; Laera, Stefania; Morelli, Sabrina; Favia, Pietro; Bader, Augustinus; Drioli, Enrico; Bartolo, Loredana De

doi:10.1016/j.biomaterials.2009.04.052

Cited by 53 publications

(41 citation statements)

References 43 publications

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“…It is in accordance with previous reports. 20,31,32 After NH 3 plasma treatment, N1s in high-resolution XPS spectra indicated that the amine group (À ÀNH 2 ) was at the most prominent composition. Meanwhile, nitrogen content increased obviously up to 6.85% at 20 min of the treatment.…”

Section: Discussionmentioning

confidence: 98%

“…It has been proved that the polymers with the amine and amide radicals promote attachment of cells in the culture. 26,31,34,[42][43][44][45] The enriched polar groups on the polymer surface can provide many sites to obtain the ECM proteins by polar interaction and hydrogen bonding 24 or directly induce the interaction of cells with the reactive groups. 45 However, unsuitable prolonging of treatment time may result in loss of new generated polar groups.…”

Section: Discussionmentioning

confidence: 99%

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Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Zhong

et al. 2011

Biopolymers

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The surface characteristics of scaffolds for bone tissue engineering must support cell adhesion, migration, proliferation, and osteogenic differentiation. In the study, poly(D,L-lactide acid) (PDLLA) scaffolds were modified by combing ammonia (NH(3) ) plasma pretreatment with Gly-Arg-Gly-Asp-Ser (GRGDS)-peptides coupling technologies. The x-ray photoelectron spectroscopy (XPS) survey spectra showed the peak of N1s at the surface of NH(3) plasma pretreated PDLLA, which was further raised after GRGDS conjugation. Furthermore, N1s and C1s in the high-resolution XPS spectra revealed the presence of -C=N(imine), -C-NH-(amine), and -C=O-NH- (amide) groups. The GRGDS conjugation increased amide groups and decreased amine groups in the plasma-treated PDLLA. Confocal microscope and high performance liquid chromatography verified the anchored peptides after the conjugation process. Bone marrow mesenchymal stem cells were co-cultured with scaffolds. Fluorescent microscope and scanning electron microscope photographs revealed the best cell adhesion in NH(3) plasma pretreated and GRGDS conjugated scaffolds, and the least attachment in unmodified scaffolds. Real-time PCR demonstrated that expression of osteogenesis-related genes, such as osteocalcin, alkaline phosphatase, type I collagen, bone morphogenetic protein-2 and osteopontin, was upregulated in the single NH(3) plasma treated and NH(3) plasma pretreated scaffolds following GRGDS conjugation. The results show that NH(3) plasma treatment promotes the conjugation of GRGDS peptides to the PDLLA scaffolds via the formation of amide linkage, and combination of NH(3) plasma treatment and peptides conjugation may enhance the cell adhesion and osteogenic differentiation in the PDLLA scaffolds. © 2011 Wiley Periodicals, Inc. Biopolymers 95: 682-694, 2011.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Zhong

et al. 2011

Biopolymers

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“…Samples were washed with PBS, fixed in paraformaldehyde, permeabilized in Triton-X100 and saturated with serum as previously described [23]. In particular, human keratinocytes were visualized for the cytoskeleton protein actin and the cytokeratin CK1 and CK18.…”

Section: Cell Morphologymentioning

confidence: 99%

Polymeric membranes modulate human keratinocyte differentiation in specific epidermal layers

Salerno

Morelli

Giordano

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…The most used and effective plasma processes to tailor the material surface properties are those at low pressure onto 2D polymer surfaces, such as plasma treatments with nonpolymerizing gases (Ar, N 2 , CO 2 , H 2 O, O 2 , NH 3 ) that allow the grafting of reactive carboxyl, hydroxyl or nitrogen-based functionalities on the surface, making it more hydrophilic and cell-adhesive [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Plasma Processing of Scaffolds for Tissue Engineering and Regenerative Medicine

Intranuovo

Gristina

Fracassi

et al. 2015

Plasma Chem Plasma Process

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Plasma processes are largely employed in the biomedical field for different kind of materials. In particular, in tissue engineering, biomaterials need to be totally integrated with biological systems in order to be employed as substitutes of artificial prostheses. Since most materials do not allow a correct integration with the biological environment, plasma processes have been demonstrated to be very versatile in altering the material surface properties in order to improve the biocompatibility of materials. The challenge is to plasma modify 3D scaffolds in order to be used for in vivo regeneration of human tissues. The correct 3D biointegration inside living tissues is the crucial objective, towards which many aspects are directed, from the material engineering to its surface modification and affinity with the biological environment. In this paper, the advances in low pressure plasma processes, applied to both 2D rigid substrates and 3D porous structures, are discussed. Further an in vivo experiment in ovine animals using plasma processed 3D scaffolds is illustrated

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Improved functions of human hepatocytes on NH3 plasma-grafted PEEK-WC–PU membranes

Cited by 53 publications

References 43 publications

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Polymeric membranes modulate human keratinocyte differentiation in specific epidermal layers

Plasma Processing of Scaffolds for Tissue Engineering and Regenerative Medicine

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